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LMBP plasmid details

Last data update: 25 September 2020 04:24 CEST

Plasmid name: pLT10lth minusTAT CrmAmut (LMBP 3998)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Cowpox virus cytokine response modifier A cDNA (crmA); mutated sequence
Promoter: Phage T7 gene 10 promoter (T7g10)
Ribosome
binding site:
-
Terminator: Phage T7 gene 10 terminator (T7g10)
Phage fd terminator
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pLT10 lth minus TAT CASP-11 c/s Etag; pCAGGS CrmA mut
Further information: The plasmid was constructed by PCR amplifying the crmA cds from pCAGGS CrmA mut, and ligating it as a NotI/KpnI fragment into the NotI/KpnI opened pLT10 lth minus TAT CASP-11 c/s Etag vector.
The nucleotide sequence of the wild type crmA cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M14217.1.
EMBL Accession number: M14217.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 09/10/2013
Sequence detail:
Primers used to amplify the crmA mutant cds:

Forward : 5' CGG GTA CCC GAT ATC TTC AGG GAA ATC GCA TC
Reverse : 5' AAG GAA AAT AGC GGC CGC ATT AGT TGT TGG AGA GCA ATA TCT AC
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061(λ)
Host reference: Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
Related host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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