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LMBP plasmid details

Last data update: 26 September 2020 04:22 CEST

Plasmid name: pLT10lth TAT CASP11c/sEtag (LMBP 3997)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse cysteinyl aspartate specific proteinase 11 cDNA (caspase-11, CASP-11, Ich3, Casp4); mutated sequence C/S
Promoter: Phage T7 gene 10 promoter (T7g10)
Ribosome
binding site:
-
Terminator: Phage T7 gene 10 terminator (T7g10)
Phage fd terminator
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pLT10lth minusTAT CASP11c/sEtag
Further information: The plasmid was constructed by cloning an adaptor fragment into the EheI/KpnI opened pLT10lth minus TAT CASP-11 c/s Etag plasmid.
EMBL Accession number: -
Latest sequence update: 09/10/2013
Sequence detail:
Adaptor sequence:

5' GCC A TAT GGA CGA AAA AAA CGC CGG CAA CGG CGA CGA GCG GTA C 3'
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061(λ)
Host reference: Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
Related host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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