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LMBP plasmid details

Last data update: 26 September 2020 04:22 CEST

Plasmid name: pLT10TH-F-MALT1Cd80-GyrB (LMBP 6105)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: -
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human MALT1 paracaspase cDNA (MALT1, MLT, PCASP1, GeneID 10892); fragment
Escherichia coli DNA gyrase subunit B (gyrB); fragment
Enterokinase cleavage site (EK); N-terminal
FLAG epitope tag; N-terminal
Histidine tag (His-tag); N-terminal
Promoter: Phage λ major leftward promoter (λ PL)
Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10)
Terminator: Phage fd terminator
Phage T7 gene 10 terminator (T7g10)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pLT10TH; pCD-LCK-HLH-F-MALT1Cd80-GyrB
Further information: The plasmid was constructed by isolating the FLAG-tagged minimal active domain of human MALT1, fused to the E. coli Gyrase B dimerization domain, from pCD-LCK-HLH-F-MALT1Cd80-GyrB, and cloning it into the pLT10TH vector.
This clone is used for mapping of minimal active domain of MALT1 for proteolytic activity.
The nucleotide sequence of the human MALT1 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AF130356.2.
EMBL Accession number: AF130356.2, view at EMBL, GenBank, DDBJ
Latest sequence update: 07/04/2009
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli B BL21-CodonPlus-RIL[pT7POL26]
Host reference: -
Helper plasmid: pT7POL26
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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