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LMBP plasmid details

Last data update: 23 September 2020 04:27 CEST

Plasmid name: pLT10TH-F-MALT1C-C539A-GyrB (LMBP 6104)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: -
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human MALT1 paracaspase cDNA (MALT1, MLT, PCASP1, GeneID 10892); mutated fragment
Escherichia coli DNA gyrase subunit B (gyrB); fragment
Enterokinase cleavage site (EK); N-terminal
FLAG epitope tag; N-terminal
Histidine tag (His-tag); N-terminal
Promoter: Phage λ major leftward promoter (λ PL)
Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10)
Terminator: Phage fd terminator
Phage T7 gene 10 terminator (T7g10)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pLT10TH; pCD-LCK-HLH-F-MALT1C-C539A-GyrB
Further information: The plasmid was constructed by isolating the FLAG-tagged mutated human MALT1 fragment, fused to the E. coli Gyrase B dimerization domain, from pCD-LCK-HLH-F-MALT1C-C539A-GyrB, and cloning it into the pLT10TH vector.
The plasmid contains a C-terminal fragment of human MALT1, consisting of codons 334 up to and including 824, and contains a C539A mutation.
The nucleotide sequence of the human MALT1 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AF130356.2.
EMBL Accession number: AF130356.2, view at EMBL, GenBank, DDBJ
Latest sequence update: 07/04/2009
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli B BL21-CodonPlus-RIL[pT7POL26]
Host reference: -
Helper plasmid: pT7POL26
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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