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LMBP plasmid details

Last data update: 26 September 2020 04:22 CEST

Plasmid name: pLT10T3-His-TAT-mABIN-1f-E (LMBP 4230)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p4230.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human immunodeficiency virus (HIV) trans-activator of transcription cDNA (TAT); protein transduction domain
Mouse A20-binding inhibitor of NF-κB activation 1 cDNA (ABIN-1, TNIP1); minimal NF-κB inhibiting fragment
Histidine tag (His-tag); N-terminal
E-tag; C-terminal
Promoter: Phage λ major leftward promoter (λ PL)
Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10)
Terminator: Phage T7 gene 10 terminator (T7g10)
Phage fd terminator
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: -
Further information: This plasmid was constructed by inserting an XmaI-NotI PCR amplicon, containing a fusion gene between a fragment of the HIV TAT cDNA and the minimal NF-κB inhibiting mouse ABIN-1 cDNA fragment that consists of nucleotides 1446 to 1919 of the coding sequence, flanked by the N-terminal His-tag and the C-terminal E-tag.
The plasmid is meant for procaryotic expression of the mABIN gene.
Other name of the plasmid is pLT10T3His-ABIN(1446-1919)-E.
EMBL Accession number: -
Latest sequence update: 30/03/2009
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Schwarze et al., Science 285 (1999), 1569-1572 [PMID: 10477521]
Nagahara et al., Nat. Med. 12 (1998), 1449-1452 [PMID: 9846587]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061(λ)
Host reference: Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
Related host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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