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LMBP plasmid details

Last data update: 04 December 2020 04:17 CET

Plasmid name: pLT10T3-E-mABIN-1(54-647) (LMBP 5390)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse A20-binding inhibitor of NF-κB activation 1 cDNA (ABIN-1, TNIP1); fragment encoding amino acids 54-647
E-tag; N-terminal
Promoter: Phage λ major leftward promoter (λ PL)
Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10)
Terminator: Phage fd terminator
Phage T7 gene 10 terminator (T7g10)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pLT10T3-E-ICE
Further information: The mABIN-1 (54-647) fragment was PCR amplified and cloned as a NotI-SmaI fragment into the NotI-SmaI opened pLT10T3-E-ICE vector.
EMBL Accession number: AJ242778, view at EMBL, GenBank, DDBJ
Latest sequence update: 05/09/2007
Sequence detail:
PCR primers used to isolate the mABIN-1 (54-647) fragment:

                                          54
mABIN-1 AZ54 FWD NotI: ATAAGAATGCGGCCGCG.ATG.GAA.GCG.TCC.AGA.CTC.C
                                 NotI    

mABIN-1 AZ647 REV SmaI: TCCCCCGGGTCTTG.TCA.ACA.GTT.CGA.GG
                            SmaI       +++

+++: termination codon
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr K. Heyninck(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061(λ)
Host reference: Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
Related host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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