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LMBP plasmid details

Last data update: 26 September 2020 04:22 CEST

Plasmid name: pLR1HIFNG5 (LMBP 699)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p699.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human interferon γ cDNA (IFNG)
Promoter: Phage λ major leftward promoter (λ PL)
Phage λ major rightward promoter (λ PR)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLc2833
Further information: The plasmid was constructed by cloning the human interferon gamma (HIFNG) cDNA, including the original signal sequence, as a dC/dG-tailed BamHI fragment into the BamHI opened pPLc2833 vector.
The cloned hIFNG cDNA carries a dC/DG-tail at the 5' end as well as at the 3' end; the length of the dC/dG-tails is not known.
The nucleotide sequence at the 3' end of the hIFNG cDNA insert has not been experimentally verified.
Other name of the plasmid is pLRgamma5.
EMBL Accession number: -
Latest sequence update: 10/03/1994
Sequence detail:
Nucleotide sequence of the hybrid lambda PL/PR promoter.


     OL2                        OR1           
TATCTCTGGCGGTGTT|GACTATTT TACCTCTGGCGGTGATA ATGGTTGCATGTGGAATTC
             HindII                                 *


OL2: Left operator
OR1: Rigth operator
|  : HindII fusion between lambda PL and PR sequences
*  : Start of transcription
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 GM119(λ)
Host reference: Arraj et al., J. Bacteriol. 153 (1983), 562-565 [PMID: 6336742]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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