Last data update: 15 January 2021 04:14 CET
Plasmid name: pLR1HIFNG5 (LMBP 699)
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p699.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: | Human interferon γ cDNA (IFNG) |
Promoter: | Phage λ major leftward promoter (λ PL) Phage λ major rightward promoter (λ PR) |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
Parental clone: | pPLc2833 |
Further information: | The plasmid was constructed by cloning the human interferon gamma (HIFNG) cDNA, including the original signal sequence, as a dC/dG-tailed BamHI fragment into the BamHI opened pPLc2833 vector. The cloned hIFNG cDNA carries a dC/DG-tail at the 5' end as well as at the 3' end; the length of the dC/dG-tails is not known. The nucleotide sequence at the 3' end of the hIFNG cDNA insert has not been experimentally verified. Other name of the plasmid is pLRgamma5. |
EMBL Accession number: | - |
Latest sequence update: | 10/03/1994 |
Sequence detail: | Nucleotide sequence of the hybrid lambda PL/PR promoter. OL2 OR1 TATCTCTGGCGGTGTT|GACTATTT TACCTCTGGCGGTGATA ATGGTTGCATGTGGAATTC HindII * OL2: Left operator OR1: Rigth operator | : HindII fusion between lambda PL and PR sequences * : Start of transcription |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 GM119(λ) |
Host reference: | Arraj et al., J. Bacteriol. 153 (1983), 562-565 [PMID: 6336742] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 28°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.