Last data update: 26 September 2020 04:22 CEST
Plasmid name: pLR1HIFNG5 (LMBP 699)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Human interferon γ cDNA (IFNG)|
|Promoter:||Phage λ major leftward promoter (λ PL)
Phage λ major rightward promoter (λ PR)
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; use strains with a cI function, cIts for PL controlled expression|
|Further information:||The plasmid was constructed by cloning the human interferon gamma (HIFNG) cDNA, including the original signal sequence, as a dC/dG-tailed BamHI fragment into the BamHI opened pPLc2833 vector.
The cloned hIFNG cDNA carries a dC/DG-tail at the 5' end as well as at the 3' end; the length of the dC/dG-tails is not known.
The nucleotide sequence at the 3' end of the hIFNG cDNA insert has not been experimentally verified.
Other name of the plasmid is pLRgamma5.
|EMBL Accession number:||-|
|Latest sequence update:||10/03/1994|
Nucleotide sequence of the hybrid lambda PL/PR promoter. OL2 OR1 TATCTCTGGCGGTGTT|GACTATTT TACCTCTGGCGGTGATA ATGGTTGCATGTGGAATTC HindII * OL2: Left operator OR1: Rigth operator | : HindII fusion between lambda PL and PR sequences * : Start of transcription
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 GM119(λ)|
|Host reference:||Arraj et al., J. Bacteriol. 153 (1983), 562-565 [PMID: 6336742]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.