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LMBP plasmid details

Last data update: 04 December 2020 04:17 CET

Plasmid name: pLNR2 (LMBP 1518)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: plnr2.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Phage λ N gene
Phage λ cI temperature-sensitive repressor gene (cIts, cI857)
Promoter: Phage λ major rightward promoter (λ PR)
Phage λ major leftward promoter (λ PL)
Phage λ promoter for repressor maintenance (λ PRM)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage λ N gene
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pAT153cI857
Further information: The plasmid was constructed by cloning a λ BglII-BamHI fragment (containing the PL promoter and coding region of the N gene) in the BamHI site from pAT153cI857. The orientation of the inserted N gene is opposite to that of the cI857 temperature sensitive repressor gene.
The λ PR promoter is controlled by the cI857 temperature sensitive repressor that is cloned on the same plasmid.
The λ PR promoter can be used for the expression of fragments containing a ribosome binding site functional in E. coli.
Expression of the λ N gene is controlled by PL promoter and cI857 repressor.
The length of the G/C tails after the cI857 gene is not known.
EMBL Accession number: -
Latest sequence update: 25/03/2011
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Mertens et al., Biotechnology 13 (1995), 175-179 [PMID: 9634760]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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