Last data update: 04 December 2020 04:17 CET
Plasmid name: pLNR2 (LMBP 1518)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Phage λ N gene
Phage λ cI temperature-sensitive repressor gene (cIts, cI857)
|Promoter:||Phage λ major rightward promoter (λ PR)
Phage λ major leftward promoter (λ PL)
Phage λ promoter for repressor maintenance (λ PRM)
|Ribosome binding site (RBS) of the phage λ N gene|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Further information:||The plasmid was constructed by cloning a λ BglII-BamHI fragment (containing the PL promoter and coding region of the N gene) in the BamHI site from pAT153cI857. The orientation of the inserted N gene is opposite to that of the cI857 temperature sensitive repressor gene.
The λ PR promoter is controlled by the cI857 temperature sensitive repressor that is cloned on the same plasmid.
The λ PR promoter can be used for the expression of fragments containing a ribosome binding site functional in E. coli.
Expression of the λ N gene is controlled by PL promoter and cI857 repressor.
The length of the G/C tails after the cI857 gene is not known.
|EMBL Accession number:||-|
|Latest sequence update:||25/03/2011|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Mertens et al., Biotechnology 13 (1995), 175-179 [PMID: 9634760]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.