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LMBP plasmid details

Last data update: 25 September 2020 04:24 CEST

Plasmid name: pLM20 (LMBP 9306)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9306.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Yersinia enterocolitica secretion protein P gene (yscP); modified sequence (yscP388)
Yersinia enterocolitica secretion protein P gene (yscP); modified sequence (yscP686)
Escherichia coli lac repressor gene (lacI)
Histidine tag (His-tag); N-terminal
Promoter: Phage T7 gene 10 promoter (T7g10) with lac operator (T7lac)
Escherichia coli lac repressor promoter (lacI)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)
Terminator: Phage T7 gene 10 terminator (T7g10)
Selection marker: Streptomycin (Sm; spectinomycin (Sp))
Replicon: Enterobacter cloacae plasmid ClDF13 origin
Host range: Escherichia coli
Gram-negative bacterial strains
Parental clone: pCDFDuet; pLM21
Further information: The plasmid was constructed by cloning the Y. enterocolitica yscP686 CDS of pLM21 into the NdeI/XhoI opened pCDFDuet vector.
Y. enterocolitica yscP388 is a derivative of yscP in which the spacer between the two export signals and one copy of the repeats in the central part have been removed.
Y. enterocolitica yscP686 is a derivative of yscP in which an additional copy of codons 214-374 has been inserted between codons 250 and 251.
Other name of the plasmid is pDUET: yscp388+,yscP686+.
EMBL Accession number: -
Latest sequence update: 03/07/2014
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr G. Cornelis(1). It was constructed by Dr L. Metzger(2).
(1) Research Unit in Microorganism Biology, University of Namur, Namur, Belgium
(2) Biozentrum, University of Basel, Switzerland
Plasmid reference: Wagner et al., Proc. Natl. Acad. Sci. U.S.A. 107 (2010), 13860-13865 [PMID: 20643949]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 LK111
Host reference: Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257]
Cultivation medium: LB-Lennox + streptomycin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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