Last data update: 04 December 2020 04:17 CET
Plasmid name: pLM20 (LMBP 9306)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Yersinia enterocolitica secretion protein P gene (yscP); modified sequence (yscP388)
Yersinia enterocolitica secretion protein P gene (yscP); modified sequence (yscP686)
Escherichia coli lac repressor gene (lacI)
Histidine tag (His-tag); N-terminal
|Promoter:||Phage T7 gene 10 promoter (T7g10) with lac operator (T7lac)
Escherichia coli lac repressor promoter (lacI)
|Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)|
|Terminator:||Phage T7 gene 10 terminator (T7g10)|
|Selection marker:||Streptomycin (Sm; spectinomycin (Sp))|
|Replicon:||Enterobacter cloacae plasmid ClDF13 origin|
|Host range:||Escherichia coli
Gram-negative bacterial strains
|Parental clone:||pCDFDuet; pLM21|
|Further information:||The plasmid was constructed by cloning the Y. enterocolitica yscP686 CDS of pLM21 into the NdeI/XhoI opened pCDFDuet vector.
Y. enterocolitica yscP388 is a derivative of yscP in which the spacer between the two export signals and one copy of the repeats in the central part have been removed.
Y. enterocolitica yscP686 is a derivative of yscP in which an additional copy of codons 214-374 has been inserted between codons 250 and 251.
Other name of the plasmid is pDUET: yscp388+,yscP686+.
|EMBL Accession number:||-|
|Latest sequence update:||03/07/2014|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr G. Cornelis(1). It was constructed by Dr L. Metzger(2).
(1) Research Unit in Microorganism Biology, University of Namur, Namur, Belgium
(2) Biozentrum, University of Basel, Switzerland
|Plasmid reference:||Wagner et al., Proc. Natl. Acad. Sci. U.S.A. 107 (2010), 13860-13865 [PMID: 20643949]
|Restricted distribution:||- BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 LK111|
|Host reference:||Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257]
|Cultivation medium:||LB-Lennox + streptomycin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.