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LMBP plasmid details

Last data update: 25 September 2020 04:24 CEST

Plasmid name: pLK15 (LMBP 1912)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: plk15.gb (View with Genome Compiler)
plk15.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Phage fd gene 3 (g3)
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage fd gene 3
Terminator: -
Selection marker: Kanamycin (kan)
Replicon: Escherichia coli plasmid ColE1 origin
Host range: Escherichia coli; use strains with a cI function
Parental clone: pPLa2311
Further information: Gene 3 of fd phage was first cloned as an AluI-AluI fragment into the HindII site of pUR222; then the cloned gene was inserted in pPLa2311 as a PstI-EcoRI fragment.
No repression of P1 is needed; the fd-3 protein is not toxic and can be constitutively expressed.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727597.1.
EMBL Accession number: LT727597.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 11/10/1989
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRI and HindII.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr P. Bross(1).
(1) University of Konstanz
Plasmid reference: Bross et al., J. Gen. Microbiol. 134 (1988), 461-471 [PMID: 3171545]
Related plasmid reference: Beck et al., Nucleic Acids Res. 5 (1978), 4495-4503 [PMID: 745987]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + kanamycin (50 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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