Last data update: 26 September 2020 04:22 CEST
Plasmid name: pLJM32 (LMBP 9311)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Yersinia enterocolitica outer membrane adhesin protein gene (yadA, P1, yopA)|
|Promoter:||Escherichia coli lac operon promoter
Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
|Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)|
|Selection marker:||Neomycin (neo; kanamycin (kan))|
|Replicon:||Broad-host-range Gram-negative Bordetella bronchiseptica S87 plasmid pBBR1 replicon|
|Host range:||Escherichia coli
Gram-negative bacterial strains
|Parental clone:||pBBR1MCS-2; pMS8|
|Further information:||The plasmid was constructed by cloning a 3.2 kb BglII/XmnI fragment from pMS8, containing the entire yadA gene from Y. enterocolitica 439-80 and its promoter, into the BamHI/EcoRV opened pBBR1MCS-2.
Other names of the plasmid are pBBRMCS2:yadA wt and pYadAWT.
|EMBL Accession number:||-|
|Latest sequence update:||03/07/2014|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr G. Cornelis(1). It was constructed by Dr J. Mota(2).
(1) Research Unit in Microorganism Biology, University of Namur, Namur, Belgium
(2) Biozentrum, University of Basel, Switzerland
|Plasmid reference:||Mota et al., Science 307 (2005), 1278 [PMID: 15731447]
|Restricted distribution:||- BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 LK111|
|Host reference:||Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257]
|Cultivation medium:||LB-Lennox + kanamycin (50 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.