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LMBP plasmid details

Last data update: 26 September 2020 04:22 CEST

Plasmid name: pLJ6 (LMBP 9313)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9313.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Yersinia enterocolitica secretion protein P gene (yscP)
Promoter: Escherichia coli araBAD promoter (PBAD)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli L-ribulokinase gene (araB); optimized sequence
Terminator: Escherichia coli rrnB operon T1 terminator
Escherichia coli rrnB operon T2 terminator
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBAD/Myc-HisA
Further information: The plasmid was constructed by cloning the Y. enterocolitica yscP CDS into the NcoI/EcoRI opened pBAD/Myc-HisA vector.
Other names of the plasmid are pBAD:yscPwt+ and pBAD/Myc-HisA-yscP.
EMBL Accession number: -
Latest sequence update: 03/07/2014
Sequence detail:
Primers used to amplify the yscP CDS:

Forward: 5' GATCCC.ATG.GCC.AAT.AAA.ATC.ACC.ACT.CGT
                   ***
                NcoI

Reverse: 5' GATCGAATTC.TTA.TTC.TTC.AGC.CTC.CCA.CTC
                EcoRI  +++

***: starting codon.
+++: termination codon.
Punctuation indicates reading frame.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr G. Cornelis(1). It was constructed by Dr L. Journet(2).
(1) Research Unit in Microorganism Biology, University of Namur, Namur, Belgium
(2) Biozentrum, University of Basel, Switzerland
Plasmid reference: Journet et al., Science 302 (2003), 1757-1760 [PMID: 14657497]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 LK111
Host reference: Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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