LMBP plasmid details

Last data update: 04 December 2020 04:17 CET

Plasmid name: pLHX32-hRIP4 (LMBP 7899)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence:
analysis results


Cloned DNA: Human receptor-interacting serine-threonine kinase 4 cDNA (RIPK4, RIP4, ANKRD3, ANKK2, DIK, PKK)
Histidine tag (His-tag); N-terminal
Escherichia coli thioredoxin (TRX, trxA)
Promoter: Phage T3 promoter
Phage T7 gene 10 promoter (T7g10)
Phage λ major leftward promoter (λ PL)
binding site:
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10)
Terminator: Phage T7 gene 10 terminator (T7g10)
Phage fd terminator
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pLHX32
Further information: The plasmid was constructed by cloning the human RIPK4 coding sequence into the pLHX32 vector.
This low copy number plasmid can be used for IPTG-inducible expression of His-tagged human RIPK4 after transformation into an E. coli strain containing a compatible piCA plasmid. The His-tagged RIPK4 is not fused to trxA.
Expression of human RIPK4 can be offered by the Protein Service Facility (PSF), Inflammation Research Center, VIB-Ghent University, Belgium. Please contact BCCM/GeneCorner for your protein expression request or refer to BCCM/GeneCorner when contacting PSF directly.
The nucleotide sequence of the human RIP4 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number BC110617.1.
Other names of the plasmid are pLT32HXhRIP4 and pLHXhRIP4 kl 1a dd16_06.
EMBL Accession number: BC110617.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 24/06/2015
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr P. De Groote(1) (2) and Prof. Dr W. Declercq(1) (2).
(1) Inflammation Research Center, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: PhD thesis Philippe De Groote (2012)
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: LMBP 05343

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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