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LMBP plasmid details

Last data update: 26 September 2020 04:22 CEST

Plasmid name: pLHX32 (LMBP 6312)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p6312.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Eschericia coli thioredoxin (TRX, trxA)
Histidine tag (His-tag); N-terminal
Promoter: Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
-
Terminator: Phage T7 gene 10 terminator (T7g10)
Phage fd terminator
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pLT32
Further information: The plasmid is meant for the expression of His-tagged proteins, fused to the E. coli TRX via an enterokinase recognition site.
EMBL Accession number: -
Latest sequence update: 25/03/2009
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by J. Haustraete(1).
(1) Inflammation Research Center, VIB, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061(λ)
Host reference: Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
Related host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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