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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pKai-FcgRIV-VHH-M2e-VHH (LMBP 12097)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: -
Sequence
analysis results
Genecorner:

-

Cloned DNA: Llama anti-mouse Fcɣ receptor IV (FcɣRIV) VHH single domain antibody
Llama anti-influenza A matrix protein 2 (M2), ectodomain (M2e) VHH single domain antibody
Histidine tag (His-tag); C-terminal
Saccharomyces cerevisiae α-mating factor 1 gene (MFα1, GeneID 855914); prepro secretion signal sequence (ppMF)
Promoter: Pichia pastoris alcohol oxidase 1 promoter (AOX1)
Saccharomyces cerevisiae translation elongation factor 1α promoter (TEF1α)
Synthetic prokaryotic EM7 promoter
Ribosome
binding site:
-
Terminator: Pichia pastoris alcohol oxidase 1 terminator (AOX1)
Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)
Selection marker: Bleomycin (bleo; zeomycin (zeo; Zeocin); phleomycin (phleo))
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Pichia pastoris; integrative
Parental clone: pKai61
Further information: The plasmid was constructed by PCR amplifying the Llama anti-mouse FcɣRIV and the Llama anti-influenza M2e VHH encoding sequences and cloning them as fusion genes into the XhoI/SpeI opened pKai61 vector.
The vectors is meant to be linearized by PmeI and transformed into P. pastoris.
Other name of the plasmid is pKai_FcgRIV VHH-M2e VHH.
EMBL Accession number: -
Latest sequence update: 17/08/2020
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr X. Saelens(1)(2). It was constructed by Dr D. De Vlieger(1)(2).
(1) VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium
(2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium
Plasmid reference: De Vlieger et al., Front Immunol 10 (2019), 2920 [PMID: 31921179] [DOI: 10.3389/fimmu.2019.02920]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5αT1R
Host reference: -
Cultivation medium: LB-Lennox + zeocin (25 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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