Last data update: 05 March 2021 04:23 CET
Plasmid name: pKai-FcgRIIIa-VHH-M2e-VHH (LMBP 12096)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Llama anti-mouse Fcɣ receptor III (FcɣRIII) VHH single domain antibody
Llama anti-influenza A matrix protein 2 (M2), ectodomain (M2e) VHH single domain antibody
Histidine tag (His-tag); C-terminal
Saccharomyces cerevisiae α-mating factor 1 gene (MFα1, GeneID 855914); prepro secretion signal sequence (ppMF)
|Promoter:||Pichia pastoris alcohol oxidase 1 promoter (AOX1)
Saccharomyces cerevisiae translation elongation factor 1α promoter (TEF1α)
Synthetic prokaryotic EM7 promoter
|Terminator:||Pichia pastoris alcohol oxidase 1 terminator (AOX1)
Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)
|Selection marker:||Bleomycin (bleo; zeomycin (zeo; Zeocin); phleomycin (phleo))|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli
Pichia pastoris; integrative
|Further information:||The plasmid was constructed by PCR amplifying the Llama anti-mouse FcɣRIII and the Llama anti-influenza M2e VHH encoding sequences and cloning them as fusion genes into the XhoI/SpeI opened pKai61 vector.
The vectors is meant to be linearized by PmeI and transformed into P. pastoris.
Other name of the plasmid is pKai_FcgRIIIa VHH-M2e VHH.
|EMBL Accession number:||-|
|Latest sequence update:||17/08/2020|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr X. Saelens(1)(2). It was constructed by Dr D. De Vlieger(1)(2).
(1) VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium
(2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium
|Plasmid reference:||De Vlieger et al., Front Immunol 10 (2019), 2920 [PMID: 31921179] [DOI: 10.3389/fimmu.2019.02920]
|Restricted distribution:||- BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH5αT1R|
|Cultivation medium:||LB-Lennox + zeocin (25 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.