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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pKDsgRNA-FRT (LMBP 11104)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: -
Sequence
analysis results
Genecorner:

-

Cloned DNA: Guide RNA targeting the flippase recognition target site (FRT)
Escherichia coli plasmid pSC101 replication protein A (repA)
Escherichia coli DNA-binding transcriptional regulator AraC (araC)
Escherichia coli host-nuclease inhibitor protein Gam (gam)
Escherichia coli phage recombination protein Bet (bet)
Escherichia coli exonuclease (exo)
Promoter: Escherichia coli β-lactamase promoter (amp)
Escherichia coli tetracycline resistance promoter (tet)
Escherichia coli L-arabinose operon promoter (araB, araBAD, BAD)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp)
Terminator: Escherichia coli rrnB operon T1 terminator
Phage λ rho-independent major leftward terminator (λ tL3)
Selection marker: Spectinomycin (Sp)
Replicon: Plasmid pSC101 origin derived from R6-5
Host range: Escherichia coli
Parental clone: pKDsgRNA-ack
Further information: The plasmid was constructed by cloning the sgRNA for FRT targeting into the pKDsgRNA-ack vector via Gibson assembly.
The plasmid contains the arabinose inducible lambda red genes.
It can be used for anhydrotetracycline inducible sgRNA-FRT expression.
EMBL Accession number: -
Latest sequence update: 02/08/2018
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr T. Swings and Prof. Dr J. Michiels(1).
(1) Centre of Microbial and Plant Genetics, Department of Microbial and Molecular Systems, KU Leuven, Leuven, Belgium
Plasmid reference: Swings et al., Nat Commun 9 (2018), 2231 [PMID: 29884781] [DOI: doi.org/10.1038/s41467-018-04651-5]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + spectinomycin (50 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: Addgene 113398

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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