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LMBP plasmid details

Last data update: 05 March 2021 04:23 CET

Plasmid name: pGEM1gHIL61 (LMBP 1955)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p1955.gb (View with Genome Compiler)
p1955.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human interleukin 6 genomic DNA (IL6); exon 1 and 2
Promoter: Human interleukin 6 promoter (IL6)
Phage T7 gene 10 promoter (T7g10)
Phage SP6 promoter
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pGEM1; pURgHIL61
Further information: pGEM1gHIL61 was constructed by inserting the genomic human interleukin 6 (hIL6) XbaI fragment, containing the 5' flanking region, the promoter, the first and second exon, the entire first intron and part of the second intron from pURgHIL61, into the unique XbaI expression site of pGEM1.
This plasmid permits the in vitro transcription of hIL6 antisense RNA from the SP6 promoter.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727606.1.
The nucleotide sequence of the genomic hIL6 DNA corresponds with the EMBL Nucleotide Sequence Database accession number AC073072 .11. However, the C nucleotide at promoter position -174 (polymorphism mutant) was changed into a G nucleotide (wild type).
Name mentioned in Haegeman et al. (1987) is SP6/26-kd gDNA.
EMBL Accession number: AC073072.11, view at EMBL, GenBank, DDBJ
LT727606.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 29/04/2004
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: HindIII, HindIII/XhoI, NheI, SphI, StyI, XhoI and XmnI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Haegeman et al., Arch. Intern. Physiol. Biochim. 95 (1987), B207
Related plasmid reference: Haegeman et al., Eur. J. Biochem. 159 (1986), 625-632 [PMID: 3758081]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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