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LMBP plasmid details

Last data update: 05 March 2021 04:23 CET

Plasmid name: pENTR3C-strep-hMLKL(211-471)-S358D (LMBP 8705)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: -
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human mixed lineage kinase domain like pseudokinase cDNA (MLKL, GeneID 197259); mutated fragment
Strep-tag II; N-terminal
Promoter: -
Ribosome
binding site:
-
Terminator: Escherichia coli rrnB operon T1 terminator
Escherichia coli rrnB operon T2 terminator
Selection marker: Neomycin (neo; kanamycin (kan))
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pENTR3C
Further information: This Gateway entry plasmid was constructed by introducing an S358D mutation in the human MLKL coding sequence, and cloning a C-terminal fragment, consisting of codons 211 up to and including 471, into the pENTR3C vector.
The human MLKL fragment in this plasmid does not include a termination codon, allowing for C-terminal tagging. An N-terminal Strep-tag II was fused to the human MLKL coding sequence via PCR.
The nucleotide sequence of the wild type human MLKL coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AK091708.1.
Other name of the plasmid is pENTR3C-strep-hMLKL(211-471)-S358D-stop.
EMBL Accession number: AK091708.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 05/10/2016
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr Y. Dondelinger(1) (2) and Prof. Dr M. Bertrand(1) (2).
(1) Inflammation Research Center, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Dondelinger et al., Cell Rep 7 (2014), 971-981 [PMID: 24813885] [DOI: 10.1016/j.celrep.2014.04.026]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + kanamycin (50 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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