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LMBP plasmid details

Last data update: 17 January 2021 04:22 CET

Plasmid name: pENTR3C-hARMCX1-Stop (LMBP 7913)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p7913.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human armadillo repeat containing X-linked 1 cDNA (ARMCX1, ALEX1, GASP7)
Promoter: -
Ribosome
binding site:
-
Terminator: Escherichia coli rrnB operon T1 terminator
Escherichia coli rrnB operon T2 terminator
Selection marker: Neomycin (neo; kanamycin (kan))
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pENTR3C; IRAUp969E0920D
Further information: The plasmid was constructed by PCR amplifying the human ARMCX1 coding sequence from IMAGE clone IRAUp969E0920D, without a termination codon, and cloning it into the pENTR3C vector.
This is a Gateway entry vector, allowing for the transfer of the human ARMCX1 coding sequence, without a termination codon, via Gateway recombination.
Other name of the plasmid is pENTR3ChARMCX1-S.
EMBL Accession number: -
Latest sequence update: 24/06/2015
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr P. De Groote(1) (2) and Prof. Dr W. Declercq(1) (2).
(1) Inflammation Research Center, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: PhD thesis Philippe De Groote (2012)
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + kanamycin (50 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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