Last data update: 17 January 2021 04:22 CET
Plasmid name: pBluescriptIISK(-)-rob (LMBP 7839)
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p7839.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: | Rattus norvegicus leptin cDNA (LEP, OB) |
| Promoter: | Phage T7 gene 10 promoter (T7g10) Phage T3 promoter Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli; use strains expressing the lacZ ω fragment (lacZΔM15) for screening |
| Parental clone: | pBluescriptIISK(-) |
| Further information: | The nucleotide sequence of the rat LEP cds and its 5' and 3' untranslated regions corresponds with the Genbank accession number NM_013076.3. The region between the T7 promoter and the T3 promoter was sequenced by LMBP. Other name of the plasmid is pBluescript SK(-) Rat ob (cDNA). |
| EMBL Accession number: | NM_013076.3, view at GenBank |
| Latest sequence update: | 10/09/2012 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AvaI, BglI, EcoRI/XhoI and SacI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr R. Devos(1). (1) Roche Research Gent, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.