Last data update: 17 January 2021 04:22 CET
Plasmid name: pBT16 (LMBP 9600)
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9600.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: | Saccharomyces cerevisiae serine/threonine protein kinase 1 cDNA (YPK1); mutated sequence Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal |
| Promoter: | Saccharomyces cerevisiae serine/threonine protein kinase 1 promoter (YPK1) Phage T3 promoter Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae serine/threonine protein kinase 1 terminator (YPK1) |
| Selection marker: | Ampicillin (amp) Saccharomyces cerevisiae URA3; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae centromeric region 6 (CEN6)/ S. cerevisiae autonomously replicating sequence ARSH4 Phage f1 origin |
| Host range: | Escherichia coli Saccharomyces cerevisiae; ura3(-) |
| Parental clone: | pSR101; pPL220 |
| Further information: | The plasmid was constructed by amplifying the mutated S. cerevisiae YPK1 coding sequence from pPL220 and cloning it in the ClaI/MfeI opened pSR101 plasmid. The S. cerevisiae YPK1 coding sequence contains an L424G mutation. The nucleotide sequence of the wild type S. cerevisiae YPK1 cDNA, YPK1 promoter and YPK1 terminator corresponds with the EMBL Nucleotide Sequence Database accession number BK006944.2. |
| EMBL Accession number: | BK006944.2, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 05/08/2015 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr S. Moye-Rowley (1). It was constructed by B. Thommandru (1). (1) Laboratory of Molecular and cellular biology of eukaryotic stress resistance, Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, USA |
| Plasmid reference: | Khakhina et al., Eukaryotic Cell 14 (2015), 442-453 [PMID: 25724885] [DOI: 10.1128/EC.00021-15] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.