Last data update: 17 January 2021 04:22 CET
Plasmid name: pBRsE6Hy3f2 (LMBP 2965)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Escherichia coli alkaline phosphatase A gene (phoA); signal sequence
Mouse anti-hPLAP monoclonal antibody E6(Hy3,κ) cDNA; truncated mouse::human chimeric heavy chain of the F(ab')2 fragment E6(Hy3,κ)F2 (E6Hy3f2)
|Promoter:||Escherichia coli hybrid tryptophan/lacUV5 promoter (tac)|
|Ribosome binding site (RBS) of the Escherichia coli alkaline phosphatase A gene (phoA)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Parental clone:||pBRphoAE6Hf2; pSV51cE6Hy3f2|
|Further information:||The plasmid was constructed by ligation of three fragments; the large EcoRI-SalI fragment (ori and amp) and the EcoRI-ApaLI fragment (tac promoter, signal sequence of phoA, the variable domain and a fragment of the constant domain of E6H) of pBRphoAE6Hf2, with the ApaLI-SalI fragment of pSV51cE6Hy3f2 (containing part of the constant domain 1, the hinge and constant domain 2 of the cDNA of hIG3).
This plasmid is similar to pBRsE6Hy3, but the cDNA encoding the mouse::human chimeric heavy chain of E6(Hy3,κ) is not complete. After translation, E6Hy3f2 contains the first 27 amino acids of the original CH2 domain followed by 4 amino acids derived from the cloning strategy and the multi-stop-linker.
The sphoA-E6Hy3f2 fusion is in-phase.
The presence of the lacI(q) repressor gene is necessary to control the activity of the tac promoter.
Name mentioned in Flamez et al. (1995) is pBRphoAcHy3f2.
|EMBL Accession number:||-|
|Latest sequence update:||23/01/1997|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Dr K. De Sutter(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Flamez et al., J. Biotechnol. 42 (1995), 133-143 [PMID: 7576533]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061F'|
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.