Last data update: 17 January 2021 04:22 CET
Plasmid name: pBK-1 (LMBP 4707)
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4707.gb
(View with Genome Compiler) p4707.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: | BK virus DNA (BKV); fragment of the Gardner strain |
| Promoter: | BK virus early promoter (BKV early) BK virus late promoter (BKV late) |
| Ribosome binding site: |
- |
| Terminator: | BK virus polyadenylation signal (BKV polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin BK virus origin (BKV) |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pML |
| Further information: | pBK-1 was constructed by ligating the 5.1 kb BamHI-EcoRI fragment from the BK virus (BKV; Gardner strain) to the 2.6 kb BamHI-EcoRI fragment from pML. As a result, the BKV late genes were disrupted. pML (or pML-I) is a pBR322 derivative with deletion of the poison sequences (from pBR322 position 1094 to 2483). The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727166.1. |
| EMBL Accession number: | LT727166.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 09/06/1994 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI/EcoRI, EcoRI and ScaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr G. Barbanti-Brodano(1). (1) Institute of Microbiology, School of Medicine, University of Ferrara, Italy |
| Plasmid reference: | Milanesi et al., Mol. Cell. Biol. 4 (1984), 1551-1560 [PMID: 6092918] |
| Related plasmid reference: | Grossi et al., Arch. Virol. 99 (1988), 249-259 [PMID: 2835949] Lusky et al., Nature 293 (1981), 79-81 [PMID: 6267479] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.