Last data update: 17 January 2021 04:22 CET
Plasmid name: pBATEM2 (LMBP 3585)
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p3585.gb
(View with Genome Compiler) p3585.txt p3585.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: | Mouse cadherin 1 cDNA (Cdh1, E-cad, CDH1) |
| Promoter: | Chicken β-actin promoter (Ac) Herpes simplex virus (HSV) thymidine kinase promoter (TK) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pBATneo |
| Further information: | The plasmid contains mCdh1 cDNA preceded by the Ac promoter and the HSV-TK promoter, and can be used for expression in mammalian cells. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727351.1. The nucleotide sequence of the plasmid was compiled from personal data from Dr Nagafuchi (Faculty of Science, Department of Biophysics, Kyoto University, Japan), as well as from the data in Nose et al. (1988) and in Nagafuchi et al. (1987); it still contains some uncertainties. The 802 bp EcoRI fragment of the 3' untranslated region of mCdh1 was removed. The remnant of the multicloning site of pIBI76 at the 3' untranslated region of mCdh1 (nucleotide position 1772 to 1878 of pBATEM2) and the TK promoter region (nucleotide position 4900 to 5300 of pBATEM2) was sequenced at the Department of Biomedical Molecular Biology (Ghent University, Belgium). The nucleotide sequence of the Ac promoter region and of the 5' UTR of the chicken β-actin gene correspond with the EMBL Nucleotide Sequence Database accession number X00182.1. The nucleotide sequence of the mCdh1 cDNA corresponds with EMBL Nucleotide Sequence Database accession number X06115.1. |
| EMBL Accession number: | X00182.1, view at EMBL, GenBank, DDBJ X06115.1, view at EMBL, GenBank, DDBJ LT727351.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 20/09/1999 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: ClaI, EcoRI, NheI, PvuII, SacI, SmaI, SspI and XhoI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr M. Takeichi(1). (1) Faculty of Science, Department of Biophysics, Kyoto University, Japan |
| Plasmid reference: | Nose et al., Cell 54 (1988), 993-1001 [PMID: 3416359] |
| Related plasmid reference: | Nagafuchi et al., Nature 329 (1987), 341-343 [PMID: 3498123] Cachat et al., J Biol Eng 8 (2014), 26 [PMID: 25478005] [DOI: 10.1186/1754-1611-8-26] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.