Last data update: 17 January 2021 04:22 CET
Plasmid name: pBAD24-ProP-Y44F (LMBP 12184)
| Price category: | Cat. 1 (cf. price list) |
| Status: | |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: | Escherichia coli osmolyte:H(+) symporter ProP cDNA (proP, GeneID 948626); mutated sequence Escherichia coli DNA-binding transcriptional dual regulator AraC cDNA (araC, GeneID 944780) |
| Promoter: | Escherichia coli araBAD promoter (PBAD) |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBAD24-ProP |
| Further information: | The plasmid was constructed by introducing a Y44F mutation into the E. coli ProP coding sequence of pBAD24-ProP. The nucleotide sequence of the wild type E. coli ProP cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M83089.1. Other name of the plasmid is pDC269. |
| EMBL Accession number: | M83089.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 09/10/2020 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr D.E. Culham(1) and Prof. Em. Dr J.M. Wood(1). (1) Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada |
| Plasmid reference: | Keates et al., Biochemistry 49 (2010), 8847-8856 [PMID: 20828170] [DOI: 10.1021/bi101281f] |
| Related plasmid reference: | Culham et al., J. Mol. Recognit. 13 (2000), 309-322 [PMID: 10992293] [DOI: 10.1002/1099-1352(200009/10)13:5<309::AID-JMR505>3.0.CO;2-R] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 WG350 |
| Host reference: | Culham et al., J. Mol. Biol. 229 (1993), 268-276 [PMID: 8421314] [DOI: 10.1006/jmbi.1993.1030] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.