Last data update: 17 January 2021 04:22 CET
Plasmid name: p50huIL6P-luc+ (LMBP 4692)
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4692.gb
(View with Genome Compiler) p4692.txt p4692.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: | Photinus pyralis (firefly) luciferase gene (LUC); mutated coding region (LUCm; luc(+)) |
| Promoter: | Human interleukin 6 promoter (IL6); 50 bp minimal promoter |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) Synthetic polyadenylation signal (polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | p1168huIL6P-luc+ |
| Further information: | The plasmid was derived from p1168huIL6P-luc+ by removing the BamHI-SspI human IL6 promoter fragment, followed by religation after the BamHI site was filled in. As a result, the human IL6 promoter was reduced to a 50 bp minimal promoter. Furthermore, the SalI site between the SV40 polyA and the pMB1 origin was filled in. Mammalian cells can be directly transfected transiently with the plasmid. However, promoter regulation can be better evaluated when the mammalian cells are stably transfected. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727161.1. The nucleotide sequence of the genomic human IL6 DNA corresponds with the EMBL Nucleotide Sequence Database accession number AC073072.11. |
| EMBL Accession number: | AC073072.11, view at EMBL, GenBank, DDBJ LT727161.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 03/05/2004 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: NcoI, SalI, ScaI and SphI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr W. Vanden Berghe(1) and Prof. Dr G. Haegeman(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Plaisance et al., Mol. Cell. Biol. 17 (1997), 3733-3743 [PMID: 9199307] |
| Related plasmid reference: | Vanden Berghe et al., J. Biol. Chem. 273 (1998), 3285-3290 [PMID: 9452444] Vanden Berghe et al., J. Biol. Chem. 274 (1999), 32091-32098 [PMID: 10542243] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.