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LMBP plasmid details

Last data update: 17 January 2021 04:22 CET

Plasmid name: p50huIL6P-luc+ (LMBP 4692)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p4692.gb (View with Genome Compiler)
p4692.txt
p4692.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Photinus pyralis (firefly) luciferase gene (LUC); mutated coding region (LUCm; luc(+))
Promoter: Human interleukin 6 promoter (IL6); 50 bp minimal promoter
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Synthetic polyadenylation signal (polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Mammalian cells
Parental clone: p1168huIL6P-luc+
Further information: The plasmid was derived from p1168huIL6P-luc+ by removing the BamHI-SspI human IL6 promoter fragment, followed by religation after the BamHI site was filled in. As a result, the human IL6 promoter was reduced to a 50 bp minimal promoter. Furthermore, the SalI site between the SV40 polyA and the pMB1 origin was filled in.
Mammalian cells can be directly transfected transiently with the plasmid. However, promoter regulation can be better evaluated when the mammalian cells are stably transfected.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727161.1.
The nucleotide sequence of the genomic human IL6 DNA corresponds with the EMBL Nucleotide Sequence Database accession number AC073072.11.
EMBL Accession number: AC073072.11, view at EMBL, GenBank, DDBJ
LT727161.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 03/05/2004
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: NcoI, SalI, ScaI and SphI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr W. Vanden Berghe(1) and Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Plaisance et al., Mol. Cell. Biol. 17 (1997), 3733-3743 [PMID: 9199307]
Related plasmid reference: Vanden Berghe et al., J. Biol. Chem. 273 (1998), 3285-3290 [PMID: 9452444]
Vanden Berghe et al., J. Biol. Chem. 274 (1999), 32091-32098 [PMID: 10542243]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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