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LMBP plasmid details

Last data update: 29 October 2020 04:15 CET

Plasmid name: p1300rp2/bgla (LMBP 9140)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Aspergillus niger phospho-beta-glucosidase (BglA)
Promoter: Trichoderma reesei ribosomal protein 2 promoter (rp2)
Escherichia coli lac operon promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)
Terminator: Trichoderma reesei ribosomal protein 2 terminator (rp2)
Selection marker: Neomycin (neo; kanamycin (kan))
Hygromycin (hyg)
Replicon: Escherichia coli plasmid pMB1 origin
Pseudomonas plasmid pVS1 origin
Host range: Escherichia coli
Filamentous fungi
Parental clone: pCAMBIA-1300
Further information: The plasmid was constructed by PCR amplifying the promoter and the terminator of rp2 from T. reesei QM9414 genomic DNA and ligating them into the KpnI-BamHI sites and XbaI-SalI sites respectively of pCAMBIA-1300 (Beijing Ding Guo Changsheng Biotechnology Co.), generating p1300rp2. For heterologus expression of phospho-beta-glucosidase from A. niger, the 2.6-kb BglA gene was inserted into the XbaI site of p1300rp2. The signal peptide coding sequence was included in the amplified BglA fragment.
P1300rp2/bgla is useful for heterologous protein expression in T. reesei under control of a constitutive promoter.
The nucleotide sequence of the pCAMBIA-1300 vector corresponds with the EMBL Nucleotide Sequence Database accession number AF234296.1.
EMBL Accession number: AF234296.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 05/05/2014
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr R. He(1).
(1) Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China
Plasmid reference: He et al., Plasmid 70 (2013), 425-429 [PMID: 24120481] [DOI: 10.1016/j.plasmid.2013.09.004]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + kanamycin (50 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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