Last data update: 29 October 2020 04:15 CET
Plasmid name: p1300rp2/bgla (LMBP 9140)
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: | Aspergillus niger phospho-beta-glucosidase (BglA) |
| Promoter: | Trichoderma reesei ribosomal protein 2 promoter (rp2) Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | Trichoderma reesei ribosomal protein 2 terminator (rp2) |
| Selection marker: | Neomycin (neo; kanamycin (kan)) Hygromycin (hyg) |
| Replicon: | Escherichia coli plasmid pMB1 origin Pseudomonas plasmid pVS1 origin |
| Host range: | Escherichia coli Filamentous fungi |
| Parental clone: | pCAMBIA-1300 |
| Further information: | The plasmid was constructed by PCR amplifying the promoter and the terminator of rp2 from T. reesei QM9414 genomic DNA and ligating them into the KpnI-BamHI sites and XbaI-SalI sites respectively of pCAMBIA-1300 (Beijing Ding Guo Changsheng Biotechnology Co.), generating p1300rp2. For heterologus expression of phospho-beta-glucosidase from A. niger, the 2.6-kb BglA gene was inserted into the XbaI site of p1300rp2. The signal peptide coding sequence was included in the amplified BglA fragment. P1300rp2/bgla is useful for heterologous protein expression in T. reesei under control of a constitutive promoter. The nucleotide sequence of the pCAMBIA-1300 vector corresponds with the EMBL Nucleotide Sequence Database accession number AF234296.1. |
| EMBL Accession number: | AF234296.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 05/05/2014 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr R. He(1). (1) Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China |
| Plasmid reference: | He et al., Plasmid 70 (2013), 425-429 [PMID: 24120481] [DOI: 10.1016/j.plasmid.2013.09.004] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.