Last data update: 15 January 2021 04:14 CET
Plasmid name: p1168huIL6P-luc+ (LMBP 4495)
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4495.gb
(View with Genome Compiler) p4495.txt p4495.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: | Photinus pyralis (firefly) luciferase gene (LUC); mutated coding region (LUCm; luc(+)) |
| Promoter: | Human interleukin 6 promoter (IL6) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) Synthetic polyadenylation signal (polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pGL3-Basic; pBLHIL6CAT |
| Further information: | The plasmid was constructed by inserting the HindIII/XhoI (filled-in) fragment of pBLHIL6CAT, containing the human IL6 promoter, into the unique HindIII site of pGL3-Basic. Mammalian cells can be directly transfected transiently with the plasmid. However, promoter regulation can be better evaluated when the mammalian cells are stably transfected. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727230.1. The nucleotide sequence of the genomic human IL6 DNA corresponds with the EMBL Nucleotide Sequence Database accession number AC073072.11, except for the C nucleotide at promoter position -174 (polymorphism mutant) which was changed into a G nucleotide (wild type). Other name of the plasmid is p1168hu.ILP-luc+ wildtype. |
| EMBL Accession number: | AC073072.11, view at EMBL, GenBank, DDBJ LT727230.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 30/04/2004 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AatII, BamHI, HindII, HindII/XmnI, HindIII, NheI, PvuI, SalI, XbaI and XhoI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr W. Vanden Berghe(1) and Prof. Dr G. Haegeman(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Plaisance et al., Mol. Cell. Biol. 17 (1997), 3733-3743 [PMID: 9199307] |
| Related plasmid reference: | Vanden Berghe et al., J. Biol. Chem. 273 (1998), 3285-3290 [PMID: 9452444] Vanden Berghe et al., J. Biol. Chem. 274 (1999), 32091-32098 [PMID: 10542243] Rascle et al., Exp. Cell Res. 315 (2009), 76-96 [PMID: 18996370] [DOI: 10.1016/j.yexcr.2008.10.012] Waldburger et al., Arthritis Rheum. 58 (2008), 3439-3449 [PMID: 18975306] [DOI: 10.1002/art.23987] Codeluppi et al., PLoS ONE 9 (2014), e92649 [PMID: 24667246] [DOI: 10.1371/journal.pone.0092649] Bogard et al., Naunyn Schmiedebergs Arch. Pharmacol. 387 (2014), 329-339 [PMID: 24363043] [DOI: 10.1007/s00210-013-0950-4] Jin et al., PLoS ONE 13 (2018), e0193343 [PMID: 29474492] [DOI: 10.1371/journal.pone.0193343] Jin et al., Mol. Immunol. 49 (2011), 423-432 [PMID: 22030478] [DOI: 10.1016/j.molimm.2011.08.026] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.