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LMBP plasmid details

Last data update: 29 October 2020 04:15 CET

Plasmid name: p110huIL6P-luc+ (LMBP 4693)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p4693.gb (View with Genome Compiler)
p4693.txt
p4693.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Photinus pyralis (firefly) luciferase gene (LUC); mutated coding region (LUCm; luc(+))
Promoter: Human interleukin 6 promoter (IL6); 110 bp fragment
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Mammalian cells
Parental clone: pMahIL6P
Further information: This plasmid contains a 110 bp Sau96I/XhoI fragment of the human IL6 promoter upstream of the mutated firefly luciferase gene.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727162.1.
The nucleotide sequence of the genomic human IL6 DNA corresponds with the EMBL Nucleotide Sequence Database accession number AC073072.11.
EMBL Accession number: AC073072.11, view at EMBL, GenBank, DDBJ
LT727162.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 12/10/2011
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, HindII, NcoI, SalI and ScaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr W. Vanden Berghe(1) and Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Vanden Berghe et al., J. Biol. Chem. 273 (1998), 3285-3290 [PMID: 9452444]
Related plasmid reference: Vanden Berghe et al., J. Biol. Chem. 274 (1999), 32091-32098 [PMID: 10542243]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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