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BCCM/LMBP DNA library details

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Library name: Human osteosarcoma library  
Library type: cDNA
Library accession number: LMBP LIB3
Organism: Homo sapiens
Priming method: NotI-(dT)15 (unidirectional)
Vector name: pCDM8Sfi-Not
Vector accession number: /
Vector type: eukaryotic expression vector
Cloning sites: SfiI-NotI
Library coverage: 1,0 x 10E6
Average insert size: 1,23 kb
Distributed as: pooled library DNA
DNA concentration: 10 ng/µl
Volume: 10 µl
Further information: The host strain Escherichia coli K12 MC1061 or DH5α can be used for electroporation to amplify the library. The transformation mixture can then be streaked on selective solid medium (LB-Lennox + 100 µg/ml ampicillin) and cultivated at 37°C to obtain the different library clones.
History of deposit: The cDNA library was deposited by Prof. R. Contreras1.
1 Department of Molecular Biology, Ghent University, Belgium
Library reference: - [PMID: -]
Related reference: -
Restricted distribution: - BCCM MTA

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