ID pGAD424 standard; circular DNA; ; 6659 BP. DT 29-AUG-2007 DE U07647 (Clontech). CC Collection number: 3106 CC (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) CC Last modified: 29-AUG-2007 CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT terminator 5495..5597 FT /vntifkey="43" FT /label=FLP\terminator FT /note="Saccharomyces cerevisiae 2 micron plasmid (2µ) FLP terminator" FT promoter complement(3130..3213) FT /vntifkey="30" FT /label=lac\promoter FT /note="Escherichia coli lac operon promoter" FT rep_origin 5679..6659 FT /vntifkey="33" FT /label=2mu\ori FT /note="2µ origin, incl. region conferring stability (STB)" FT CDS complement(4248..5108) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene; coding sequence" FT rep_origin complement(3305..3707) FT /vntifkey="33" FT /label=pMB1\ori FT /note="Escherichia coli plasmid pMB1 origin" FT CDS complement(1546..2640) FT /vntifkey="4" FT /label=LEU2 FT /note="Saccharomyces cerevisiae LEU2 coding sequence" FT terminator 1241..1342 FT /vntifkey="43" FT /label=ADH1\terminator FT /note="Saccharomyces cerevisiae alcohol dehydrogenase terminator" FT CDS 422..829 FT /vntifkey="4" FT /label=GALad FT /note="Saccharomyces cerevisiae GAL4 activation domain" FT promoter 8..411 FT /vntifkey="29" FT /label=ADH1\promoter FT /note="Saccharomyces cerevisiae alcohol dehydrogenase promoter" SQ Sequence 6659 BP; 1902 A; 1499 C; 1367 G; 1891 t; gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc 60 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga 120 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac 180 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct 240 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga 300 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt 360 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa 420 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg 480 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt 540 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc 600 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag 660 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc 720 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata 780 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattccc 840 ggggatccgt cgacctgcag agatctatga atcgtagata ctgaaaaacc ccgcaagttc 900 acttcaactg tgcatcgtgc accatctcaa tttctttcat ttatacatcg ttttgccttc 960 ttttatgtaa ctatactcct ctaagtttca atcttggcca tgtaacctct gatctataga 1020 attttttaaa tgactagaat taatgcccat cttttttttg gacctaaatt cttcatgaaa 1080 atatattacg agggcttatt cagaagcttt ggacttcttc gccagaggtt tggtcaagtc 1140 tccaatcaag gttgtcggct tgtctacctt gccagaaatt tacgaaaaga tggaaaaggg 1200 tcaaatcgtt ggtagatacg ttgttgacac ttctaaataa gcgaatttct tatgatttat 1260 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc 1320 ttaggtttta aaacgaaaat tcttgttctt gagtaactct ttcctgtagg tcaggttgct 1380 ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca tgcccgaaat 1440 tcccctaccc tatgaacata ttccattttg taatttcgtg tcgtttctat tatgaatttc 1500 atttataaag tttatgtaca aatatcataa aaaaagagaa tctttttaag caaggatttt 1560 cttaacttct tcggcgacag catcaccgac ttcggtggta ctgttggaac cacctaaatc 1620 accagttctg atacctgcat ccaaaacctt tttaactgca tcttcaatgg ccttaccttc 1680 ttcaggcaag ttcaatgaca atttcaacat cattgcagca gacaagatag tggcgatagg 1740 gtcaacctta ttctttggca aatctggagc agaaccgtgg catggttcgt acaaaccaaa 1800 tgcggtgttc ttgtctggca aagaggccaa ggacgcagat ggcaacaaac ccaaggaacc 1860 tgggataacg gaggcttcat cggagatgat atcaccaaac atgttgctgg tgattataat 1920 accatttagg tgggttgggt tcttaactag gatcatggcg gcagaatcaa tcaattgatg 1980 ttgaaccttc aatgtaggaa attcgttctt gatggtttcc tccacagttt ttctccataa 2040 tcttgaagag gccaaaacat tagctttatc caaggaccaa ataggcaatg gtggctcatg 2100 ttgtagggcc atgaaagcgg ccattcttgt gattctttgc acttctggaa cggtgtattg 2160 ttcactatcc caagcgacac catcaccatc gtcttccttt ctcttaccaa agtaaatacc 2220 tcccactaat tctctgacaa caacgaagtc agtaccttta gcaaattgtg gcttgattgg 2280 agataagtct aaaagagagt cggatgcaaa gttacatggt cttaagttgg cgtacaattg 2340 aagttcttta cggattttta gtaaaccttg ttcaggtcta acactacctg taccccattt 2400 aggaccaccc acagcaccta acaaaacggc atcaaccttc ttggaggctt ccagcgcctc 2460 atctggaagt gggacacctg tagcatcgat agcagcacca ccaattaaat gattttcgaa 2520 atcgaacttg acattggaac gaacatcaga aatagcttta agaaccttaa tggcttcggc 2580 tgtgatttct tgaccaacgt ggtcacctgg caaaacgacg atcttcttag gggcagacat 2640 tagaatggta tatccttgaa atatatatat atattgctga aatgtaaaag gtaagaaaag 2700 ttagaaagta agacgattgc taaccaccta ttggaaaaaa caataggtcc ttaaataata 2760 ttgtcaactt caagtattgt gatgcaagca tttagtcatg aacgcttctc tattctatat 2820 gaaaagccgg ttccggcctc tcacctttcc tttttctccc aatttttcag ttgaaaaagg 2880 tatatgcgtc aggcgacctc tgaaattaac aaaaaatttc cagtcatcga atttgattct 2940 gtgcgatagc gcccctgtgt gttctcgtta tgttgaggaa aaaaataatg gttgctaaga 3000 gattcgaact cttgcatctt acgatacctg agtattccca cagttgggga tctcgactct 3060 agctagagga tcaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 3120 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 3180 tgagtgaggt aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 3240 ctgtcgtgcc agctggatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 3300 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 3360 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 3420 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 3480 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 3540 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 3600 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 3660 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 3720 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 3780 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 3840 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 3900 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 3960 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 4020 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 4080 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 4140 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 4200 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 4260 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 4320 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 4380 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 4440 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 4500 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 4560 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 4620 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 4680 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 4740 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 4800 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 4860 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 4920 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 4980 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 5040 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 5100 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 5160 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 5220 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa 5280 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc 5340 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga 5400 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg 5460 gcatcagagc agattgtact gagagtgcac cataacgcat ttaagcataa acacgcacta 5520 tgccgttctt ctcatgtata tatatataca ggcaacacgc agatataggt gcgacgtgaa 5580 cagtgagctg tatgtgcgca gctcgcgttg cattttcgga agcgctcgtt ttcggaaacg 5640 ctttgaagtt cctattccga agttcctatt ctctagctag aaagtatagg aacttcagag 5700 cgcttttgaa aaccaaaagc gctctgaaga cgcactttca aaaaaccaaa aacgcaccgg 5760 actgtaacga gctactaaaa tattgcgaat accgcttcca caaacattgc tcaaaagtat 5820 ctctttgcta tatatctctg tgctatatcc ctatataacc tacccatcca cctttcgctc 5880 cttgaacttg catctaaact cgacctctac attttttatg tttatctcta gtattactct 5940 ttagacaaaa aaattgtagt aagaactatt catagagtga atcgaaaaca atacgaaaat 6000 gtaaacattt cctatacgta gtatatagag acaaaataga agaaaccgtt cataattttc 6060 tgaccaatga agaatcatca acgctatcac tttctgttca caaagtatgc gcaatccaca 6120 tcggtataga atataatcgg ggatgccttt atcttgaaaa aatgcacccg cagcttcgct 6180 agtaatcagt aaacgcggga agtggagtca ggcttttttt atggaagaga aaatagacac 6240 caaagtagcc ttcttctaac cttaacggac ctacagtgca aaaagttatc aagagactgc 6300 attatagagc gcacaaagga gaaaaaaagt aatctaagat gctttgttag aaaaatagcg 6360 ctctcgggat gcatttttgt agaacaaaaa agaagtatag attctttgtt ggtaaaatag 6420 cgctctcgcg ttgcatttct gttctgtaaa aatgcagctc agattctttg tttgaaaaat 6480 tagcgctctc gcgttgcatt tttgttttac aaaaatgaag cacagattct tcgttggtaa 6540 aatagcgctt tcgcgttgca tttctgttct gtaaaaatgc agctcagatt ctttgtttga 6600 aaaattagcg ctctcgcgtt gcatttttgt tctacaaaat gaagcacaga tgcttcgtt 6659 //