ID pDONR223 standard; circular DNA; ; 5005 BP. CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT terminator complement(268..295) FT /vntifkey="43" FT /label=T2\terminator FT terminator 427..470 FT /vntifkey="43" FT /label=T1\terminator FT CDS complement(1197..1502) FT /vntifkey="4" FT /label=ccdB FT CDS complement(1844..2503) FT /vntifkey="4" FT /label=chloramphenicol\resistance FT promoter complement(3003..3019) FT /vntifkey="30" FT /label=T7\promoter FT rep_origin 4725..122 FT /vntifkey="33" FT /label=pMB1\ori FT attenuator 569..801 FT /vntifkey="0" FT /label=attP1 FT attenuator complement(2751..2983) FT /vntifkey="0" FT /label=attP2 FT CDS 3252..4262 FT /vntifkey="4" FT /label=streptomycin/spectinomycin\resistance\(aadA) SQ Sequence 5005 BP; 1297 A; 1235 C; 1169 G; 1304 t; ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 60 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 120 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 180 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 240 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 300 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 360 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 420 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 480 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 540 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 600 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 660 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa 720 aaaacagact acataatact gtaaaacaca acatatccag tcactatgaa tcaactactt 780 agatggtatt agtgacctgt agtcgaccga cagccttcca aatgttcttc gggtgatgct 840 gccaacttag tcgaccgaca gccttccaaa tgttcttctc aaacggaatc gtcgtatcca 900 gcctactcgc tattgtcctc aatgccgtat taaatcataa aaagaaataa gaaaaagagg 960 tgcgagcctc ttttttgtgt gacaaaataa aaacatctac ctattcatat acgctagtgt 1020 catagtcctg aaaatcatct gcatcaagaa caatttcaca actcttatac ttttctctta 1080 caagtcgttc ggcttcatct ggattttcag cctctatact tactaaacgt gataaagttt 1140 ctgtaatttc tactgtatcg acctgcagac tggctgtgta taagggagcc tgacatttat 1200 attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca 1260 gccacttctt ccccgataac ggagaccggc acactggcca tatcggtggt catcatgcgc 1320 cagctttcat ccccgatatg caccaccggg taaagttcac gggagacttt atctgacagc 1380 agacgtgcac tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc 1440 tgtacatcca caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc 1500 atttcaccag tccctgttct cgtcagcaaa agagccgttc atttcaataa accgggcgac 1560 ctcagccatc ccttcctgat tttccgcttt ccagcgttcg gcacgcagac gacgggcttc 1620 attctgcatg gttgtgctta ccagaccgga gatattgaca tcatatatgc cttgagcaac 1680 tgatagctgt cgctgtcaac tgtcactgta atacgctgct tcatagcaca cctctttttg 1740 acatacttcg ggtatacata tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat 1800 acgcatactg ttatctggct tttagtaagc cggatccacg cgattacgcc ccgccctgcc 1860 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga 1920 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt 1980 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac 2040 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag 2100 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact 2160 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga 2220 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca 2280 tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa 2340 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct 2400 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt 2460 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc 2520 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa 2580 agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct 2640 tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag 2700 gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa cttagtcgac tacaggtcac 2760 taataccatc taagtagttg attcatagtg actggatatg ttgtgtttta cagtattatg 2820 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 2880 ctcgttcagc tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt 2940 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc 3000 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc 3060 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca 3120 gccaggacag aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt 3180 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat 3240 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta 3300 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc 3360 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca 3420 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag 3480 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc 3540 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc 3600 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc 3660 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc 3720 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta 3780 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc 3840 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg 3900 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg 3960 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc 4020 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc 4080 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca 4140 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag 4200 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat 4260 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag 4320 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 4380 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 4440 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 4500 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 4560 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 4620 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 4680 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 4740 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 4800 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 4860 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 4920 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 4980 tttgctggcc ttttgctcac atgtt 5005 //