LOCUS       pCi-C-TagBFP-DEST         6410 bp    DNA     circular      9-MAY-2016
DEFINITION  LMBP 9838.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11230094|
COMMENT     VNTDBDATE|-11230094|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCi-C-TagBFP-DEST|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT          (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 
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COMMENT                                   0.892485 0 -10 11.0073 1.16519 #139#) 
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COMMENT                                   10.8903 -10.454 0 -10 2.15684 1.16519 #141#) 
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COMMENT                                   5.22699 0 -10 8.03237 1.16519 #143#) 
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COMMENT                                   "hCMV-IE promoter and enhancer" "@N" 1 13.122 
COMMENT                                   14.254 0 -10 13.065 1.16519 #148#)) 
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COMMENT                      #150=(CGroupWidget 
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COMMENT                                    (LOGPEN 0 4 128) 6 1.16464 5.17497 5.26223) 
COMMENT                                   0.4 0.082322 1.08464 1.8 1.8 0) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
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COMMENT                                   "@N" 1 16.1443 8.70471 0 -10 4.88388 1.16519 
COMMENT                                   #151#) 
COMMENT                             #153=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 0) 2 (LOGBRUSH 0 8388736 0) 1 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   "lacUV5 promoter" "@N" 1 17.8147 5.31921 0 
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COMMENT                      #155=(CGroupWidget 
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FEATURES             Location/Qualifiers
     promoter        138..721
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     promoter        1034..1050
                     /vntifkey="30"
                     /label=T7\promoter
     attenuator      complement(1067..1191)
                     /vntifkey="0"
                     /label=attR1
     promoter        1195..1251
                     /vntifkey="30"
                     /label=lacUV5\promoter
     CDS             1300..1959
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             2280..2585
                     /vntifkey="4"
                     /label=ccdB
     attenuator      2626..2750
                     /vntifkey="0"
                     /label=attR2
     terminator      complement(3569..3665)
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      3569..3665
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      3824..4279
                     /vntifkey="33"
                     /label=f1\ori
     CDS             4718..5578
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      6119..6397
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             2782..3480
                     /vntifkey="4"
                     /label=TagBFP
BASE COUNT     1682 a      1522 c      1549 g      1657 t 
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat 
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc 
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa 
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac 
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta 
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagatcacaa gtttgtacaa 
     1081 aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca 
     1141 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca 
     1201 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt 
     1261 taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga 
     1321 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca 
     1381 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc 
     1441 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg 
     1501 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt 
     1561 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt 
     1621 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac 
     1681 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc 
     1741 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc 
     1801 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg 
     1861 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa 
     1921 ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta 
     1981 aaagccagat aacagtatgc gtatttgcgc gctcgcgaac cggtgtatac ccgaagtatg 
     2041 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc 
     2101 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa 
     2161 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga 
     2221 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa 
     2281 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac 
     2341 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc 
     2401 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct 
     2461 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg 
     2521 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa 
     2581 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata 
     2641 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg 
     2701 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt tgatctcgag 
     2761 aacttcacgc gtggtacccc gagcgagctg attaaggaga acatgcacat gaagctgtac 
     2821 atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc 
     2881 tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc 
     2941 ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag 
     3001 ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc 
     3061 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc 
     3121 ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag 
     3181 aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg 
     3241 gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacatc 
     3301 aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat 
     3361 gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac 
     3421 gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaattag 
     3481 gctctagagt cgacccgggc ggccgcttcg agcagacatg ataagataca ttgatgagtt 
     3541 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc 
     3601 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat 
     3661 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct 
     3721 ctacaaatgt ggtaaaatcg ataaggatcc gggctggcgt aatagcgaag aggcccgcac 
     3781 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc 
     3841 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 
     3901 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 
     3961 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagagcttt acggcacctc 
     4021 gaccgcaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 
     4081 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 
     4141 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 
     4201 tcggcctatt ggttaaaaaa tgagctgatt taacaaatat ttaacgcgaa ttttaacaaa 
     4261 atattaacgt ttacaatttc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat 
     4321 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 
     4381 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 
     4441 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 
     4501 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt 
     4561 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac 
     4621 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 
     4681 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 
     4741 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 
     4801 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 
     4861 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 
     4921 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 
     4981 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 
     5041 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 
     5101 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 
     5161 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 
     5221 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 
     5281 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 
     5341 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 
     5401 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 
     5461 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 
     5521 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 
     5581 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 
     5641 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 
     5701 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 
     5761 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 
     5821 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 
     5881 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 
     5941 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 
     6001 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 
     6061 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 
     6121 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 
     6181 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 
     6241 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 
     6301 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 
     6361 acggttcctg gccttttgct ggccttttgc tcacatggct cgacagatct 
//
