LOCUS       pPLc428T1HIL2           3585 bp    DNA     circular     25-FEB-2011
DEFINITION  LMBP 966.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 966
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 25-FEB-2011
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|582803510|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pPLc428T1HIL2|
COMMENT     VNTAUTHORNAME|LMBP Plasmid collection|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     CDS             61..465
                     /vntifkey="4"
                     /label=hIL2
                     /note="human interleukin 2 mature sequence"
     misc_feature    466..740
                     /vntifkey="21"
                     /label=3'\UTR\of\hIL2
     terminator      768..834
                     /vntifkey="43"
                     /label=phage\T4\gene\32\terminator
     rep_origin      complement(1170..1572)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(2113..2973)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        3339..3469
                     /vntifkey="30"
                     /label=PL\promoter
     RBS             4..58
                     /vntifkey="32"
                     /label=5'UTR\of\phage\Mu\ner\gene,\incl.\RBS
BASE COUNT      990 a       825 c       822 g       948 t 
ORIGIN
        1 ttcttacact tagttaaatt gctaacttta tagattacaa aacttaggag ggtttttacc 
       61 atggcgccta cttcaagttc tacaaagaaa acacagctac aactggagca tttactgctg 
      121 gatttacaga tgattttgaa tggaattaat aattacaaga atcccaaact caccaggatg 
      181 ctcacattta agttttacat gcccaagaag gccacagaac tgaaacatct tcagtgtcta 
      241 gaagaagaac tcaaacctct ggaggaagtg ctaaatttag ctcaaagcaa aaactttcac 
      301 ttaagaccca gggacttaat cagcaatatc aacgtaatag ttctggaact aaagggatct 
      361 gaaacaacat tcatgtgtga atatgctgat gagacagcaa ccattgtaga atttctgaac 
      421 agatggatta ccttttgtca aagcatcatc tcaacactga cttgataatt aagtgcttcc 
      481 cacttaaaac atatcaggcc ttctatttat ttaaatattt aaattttata tttattgttg 
      541 aatgtatggt ttgctaccta ttgtaactat tattcttaat cttaaaacta taaatatgga 
      601 tcttttatga ttctttttgt aagccctagg ggctctaaaa tggtttcact tatttatccc 
      661 aaaatattta ttattatgtt gaatgttaaa tatagtatct atgtagattg gttagtaaaa 
      721 ctatttaata aatttgataa gggggggggg gggggggggg gatccggtgg atgacctttt 
      781 gaatgacctt taatagatta tattactaat taattgggga ccctagaggt cccctttttt 
      841 attttaaaaa ttttttcaca aaacggttta caagcataaa gcttggctgc ctcgcgcgtt 
      901 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 
      961 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 
     1021 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta 
     1081 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 
     1141 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct 
     1201 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 
     1261 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 
     1321 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 
     1381 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 
     1441 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 
     1501 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 
     1561 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 
     1621 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 
     1681 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 
     1741 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 
     1801 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 
     1861 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 
     1921 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 
     1981 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 
     2041 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 
     2101 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 
     2161 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 
     2221 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 
     2281 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 
     2341 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 
     2401 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg 
     2461 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 
     2521 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 
     2581 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 
     2641 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 
     2701 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac 
     2761 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 
     2821 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 
     2881 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 
     2941 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attatgtaag 
     3001 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat 
     3061 tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga 
     3121 tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca 
     3181 actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg 
     3241 atggggcgat tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcagc 
     3301 gccggtgatg ccggccacga tgcgtccggc gtagaggatc tctcacctac caaacaatgc 
     3361 ccccctgcaa aaaataaatt catataaaaa acatacagat aaccatctgc ggtgataaat 
     3421 tatctctggc ggtgttgaca taaataccac tggcggtgat actgagcaca tcagcaggac 
     3481 gcactgacca ccatgaaggt gacgctctta aaattaagcc ctgaagaagg gcagcattca 
     3541 aagcagaagg ctttggggtg tgtgatacga aacgaagcat tggaa 
//
