LOCUS pLT32 3077 bp DNA circular SYN 5-NOV-2014 DEFINITION LMBP 9406. ACCESSION pLT32 SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 3077) AUTHORS Self JOURNAL Unpublished. COMMENT Accession number: LMBP 9406 (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) Last modified: 05-NOV-2014 COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDBDATE|713804831| COMMENT LSOWNER| COMMENT VNTNAME|pLT32| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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2 0 0 #159# 2082602612 100) COMMENT (LOGPEN 12 0 1277193313) 1818583649 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 "Arial") COMMENT 1.74233 0.611111 0 "Last modified: 05-NOV-2014" COMMENT "Last modified: 05-NOV-2014" 65535 0.19833 -4.85909 COMMENT 0 -10 11.1809 1.06602 Nil)) (CObjectList)) COMMENT #246=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -186 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 1414808404 COMMENT (CStringList) 1195591491 1095189315 COMMENT (CObList COMMENT #247=(CSampFrag "pLT32" 1186 1034 "HaeII" 2220 "HincII" (LOGPEN 0 0 0) COMMENT 1.24324e-316 1.02894e-314) COMMENT #248=(CSampFrag "pLT32" 817 217 "HincII" 1034 "HaeII" (LOGPEN 0 0 0) COMMENT 1.22223e-316 1.52462e-300) COMMENT #249=(CSampFrag "pLT32" 575 2220 "HincII" 2795 "HaeII" (LOGPEN 0 0 0) COMMENT 1.6976e-313 1.53406e-300) COMMENT #250=(CSampFrag "pLT32" 365 2929 "HincII" 217 "HincII" (LOGPEN 0 0 0) COMMENT 6.46578e+170 1.1695e-300) COMMENT #251=(CSampFrag "pLT32" 134 2795 "HaeII" 2929 "HincII" (LOGPEN 0 0 0) COMMENT 1.22223e-316 7.31961e-304)))) FEATURES Location/Qualifiers promoter 25..41 /gene="T7" /product="T7" /SECDrawAs="Region" /vntifkey="30" /label=T7\promoter promoter complement(249..266) /gene="T3" /product="T3" /SECDrawAs="Region" /vntifkey="30" /label=T3\promoter CDS complement (1605..2465) /gene="bla" /product="bla" /SECDrawAs="Gene" /vntifkey="4" /label=ampicillin\resistance promoter 2831..2961 /vntifkey="30" /label=PL\promoter RBS 42..104 /vntifkey="32" /label=5'\UTR\of\T7\gene\10,\incl.\RBS terminator 284..331 /vntifkey="43" /label=T7\terminator terminator 405..452 /vntifkey="43" /label=T7\terminator terminator 525..606 /vntifkey="43" /label=fd\terminator terminator 635..716 /vntifkey="43" /label=fd\terminator rep_origin complement(786..1064) /vntifkey="33" /label=pMB1\ori BASE COUNT 815 a 752 c 722 g 788 t ORIGIN 1 ttccggatct cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc 61 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc ccgacgtcgc 121 atgctcctct agactcgagg aattcggtac cccgggttcg aaatcgataa gcttatgcat 181 gcggccgcat ctagagggcc cggatccctc gaggtcgacg aattcgagct cggccgactt 241 ggccttccct ttagtgaggg ttaataaact tggtgagcaa taactagcat aaccccttgg 301 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat gcgctcatac 361 gatatgaacg ttgagactgc cgctgagtta tcagtgagca ataactagca taaccccttg 421 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggccgga 481 tagcttatcg ctagaggtcg aaattcacct cgaaagcaag ctgataaacc gatacaatta 541 aaggctcctt ttggagcctt tttttttgga gattttcaac gtgaaaaaat tattattcgc 601 aattccaagc taattcacct cgaaagcaag ctgataaacc gatacaatta aaggctcctt 661 ttggagcctt tttttttgga gattttcaac gtgaaaaaat tattattcgc aattccaagc 721 tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctccctaggc 781 aattgcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 841 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 901 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 961 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 1021 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 1081 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 1141 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 1201 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 1261 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 1321 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 1381 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 1441 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 1501 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 1561 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 1621 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 1681 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 1741 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 1801 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 1861 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 1921 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 1981 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 2041 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 2101 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 2161 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 2221 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 2281 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 2341 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 2401 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 2461 ctcatactct tcctttttca atattatgta agcagacagt tttattgttc atgatgatat 2521 atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat 2581 aaatcgaact tttgctgagt tgaaggatca gatcacgcat cttcccgaca acgcagaccg 2641 ttccgtggca aagcaaaagt tcaaaatcac caactggtcc acctacaaca aagctctcat 2701 caaccgtggc tccctcactt tctggctgga tgatggggcg attcaggcct ggtatgagtc 2761 agcaacacct tcttcacgag gcagacctca gcgccggtga tgccggccac gatgcgtccg 2821 gcgtagagga tctctcacct accaaacaat gcccccctgc aaaaaataaa ttcatataaa 2881 aaacatacag ataaccatct gcggtgataa attatctctg gcggtgttga cataaatacc 2941 actggcggtg atactgagca catcagcagg acgcactgac caccatgaag gtgacgctct 3001 taaaattaag ccctgaagaa gggcagcatt caaagcagaa ggctttgggg tgtgtgatac 3061 gaaacgaagc attggaa //