LOCUS pLT32 3077 bp DNA circular SYN 5-NOV-2014
DEFINITION LMBP 9406.
ACCESSION pLT32
SOURCE Unknown.
ORGANISM Unknown
Unclassified.
REFERENCE 1 (bases 1 to 3077)
AUTHORS Self
JOURNAL Unpublished.
COMMENT Accession number: LMBP 9406
(c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
Last modified: 05-NOV-2014
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
COMMENT ORIGDB|GenBank
COMMENT VNTDBDATE|713804831|
COMMENT LSOWNER|
COMMENT VNTNAME|pLT32|
COMMENT VNTAUTHORNAME|Demo User|
COMMENT VNTOAUTHORNAME|UNKNOWN|
COMMENT VNTREPLTYPE|Plasmid
COMMENT VNTEXTCHREPL|Bacteria
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COMMENT 6.46578e+170 1.1695e-300)
COMMENT #251=(CSampFrag "pLT32" 134 2795 "HaeII" 2929 "HincII" (LOGPEN 0 0 0)
COMMENT 1.22223e-316 7.31961e-304))))
FEATURES Location/Qualifiers
promoter 25..41
/gene="T7"
/product="T7"
/SECDrawAs="Region"
/vntifkey="30"
/label=T7\promoter
promoter complement(249..266)
/gene="T3"
/product="T3"
/SECDrawAs="Region"
/vntifkey="30"
/label=T3\promoter
CDS complement (1605..2465)
/gene="bla"
/product="bla"
/SECDrawAs="Gene"
/vntifkey="4"
/label=ampicillin\resistance
promoter 2831..2961
/vntifkey="30"
/label=PL\promoter
RBS 42..104
/vntifkey="32"
/label=5'\UTR\of\T7\gene\10,\incl.\RBS
terminator 284..331
/vntifkey="43"
/label=T7\terminator
terminator 405..452
/vntifkey="43"
/label=T7\terminator
terminator 525..606
/vntifkey="43"
/label=fd\terminator
terminator 635..716
/vntifkey="43"
/label=fd\terminator
rep_origin complement(786..1064)
/vntifkey="33"
/label=pMB1\ori
BASE COUNT 815 a 752 c 722 g 788 t
ORIGIN
1 ttccggatct cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc
61 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc ccgacgtcgc
121 atgctcctct agactcgagg aattcggtac cccgggttcg aaatcgataa gcttatgcat
181 gcggccgcat ctagagggcc cggatccctc gaggtcgacg aattcgagct cggccgactt
241 ggccttccct ttagtgaggg ttaataaact tggtgagcaa taactagcat aaccccttgg
301 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat gcgctcatac
361 gatatgaacg ttgagactgc cgctgagtta tcagtgagca ataactagca taaccccttg
421 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggccgga
481 tagcttatcg ctagaggtcg aaattcacct cgaaagcaag ctgataaacc gatacaatta
541 aaggctcctt ttggagcctt tttttttgga gattttcaac gtgaaaaaat tattattcgc
601 aattccaagc taattcacct cgaaagcaag ctgataaacc gatacaatta aaggctcctt
661 ttggagcctt tttttttgga gattttcaac gtgaaaaaat tattattcgc aattccaagc
721 tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctccctaggc
781 aattgcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
841 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
901 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
961 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
1021 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
1081 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
1141 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
1201 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
1261 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
1321 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
1381 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
1441 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
1501 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
1561 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
1621 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
1681 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
1741 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
1801 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
1861 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
1921 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
1981 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
2041 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
2101 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
2161 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
2221 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
2281 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
2341 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
2401 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
2461 ctcatactct tcctttttca atattatgta agcagacagt tttattgttc atgatgatat
2521 atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat
2581 aaatcgaact tttgctgagt tgaaggatca gatcacgcat cttcccgaca acgcagaccg
2641 ttccgtggca aagcaaaagt tcaaaatcac caactggtcc acctacaaca aagctctcat
2701 caaccgtggc tccctcactt tctggctgga tgatggggcg attcaggcct ggtatgagtc
2761 agcaacacct tcttcacgag gcagacctca gcgccggtga tgccggccac gatgcgtccg
2821 gcgtagagga tctctcacct accaaacaat gcccccctgc aaaaaataaa ttcatataaa
2881 aaacatacag ataaccatct gcggtgataa attatctctg gcggtgttga cataaatacc
2941 actggcggtg atactgagca catcagcagg acgcactgac caccatgaag gtgacgctct
3001 taaaattaag ccctgaagaa gggcagcatt caaagcagaa ggctttgggg tgtgtgatac
3061 gaaacgaagc attggaa
//