LOCUS      pMA12      7889 bp    DNA    circular    08-OCT-2008
DEFINITION  LMBP 9320.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|702570316|
COMMENT     VNTDBDATE|702570316|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pMA12|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria

FEATURES		Location/Qualifiers
	CDS 		1599..3237
			/label="strAB"
			/note="Geneious name: strAB"
	rep_origin	3276..3662
			/label="ori\R6K"
			/note="Geneious name: ori\R6K"
	CDS		complement(3669..4831)
			/label="mob\RK2/RP4"
			/note="Geneious name: mob\RK2/RP4"
	CDS		4904..5406
			/label="insB"
			/note="Geneious name: insB"
	CDS		complement(5553..6965)
			/label="sacBR"
			/note="Geneious name: sacBR"
	modified_base	3248..3249
			/label="=\dam"
			/note="Geneious name: =\dam"
	misc_feature	6..205
			/label="YscC\end"
			/note="Geneious name: YscC\end"
	misc_feature	281..985
			/label="mCherry"
			/note="Geneious name: mCherry"
	misc_feature	986..1015
			/label="RBS\for\YscD"
			/note="Geneious name: RBS\for\YscD"
	misc_feature	1012..1178
			/label="YscD\beginning"
			/note="Geneious name: YscD\beginning"
ORIGIN
        1 gtcgaccttg aataagttat taggtggctc ccagtgtcag cctttaaaca aagcgcaaga
       61 agtgcagaaa tggctgagtc aaaataataa atcatcctat cttactcagt gcaagatgga
      121 caaaagtttg ggatggcgcg tggttgaagg tgcttgtact cccgcgcaat catggtgtgt
      181 ttcagcacct aagcgtggcg tattgggtgg cgcaggcggt gccggaggta ctcgagctcc
      241 ggagaattcg aacgttacgc gtcaccggtc ggccaccatg gtgagcaagg gcgaggagga
      301 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
      361 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
      421 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
      481 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
      541 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
      601 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
      661 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
      721 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
      781 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
      841 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
      901 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
      961 cggcggcatg gacgagctgt acaaggtttc agcacctaag cgtggcgtat tgtgagttgg
     1021 gtctgtcgtt tttatcaagg gaagcaccgt ggtgttgaag ttgagcttcc tcatgggcgc
     1081 tgtgtttttg gctcagaccc gttgcaatca gatattgttc tttctgacag cgaaatagca
     1141 cccgtgcatt tagtgctgat ggtcgatgaa gaaggtattc tagagtcttt gttttgacgc
     1201 cattagcgta cgtaacaatc ctcgttaaag gacaaggacc tgagcggaag tgtatcgtac
     1261 agtagacgga gtatactagt atagtctata gtccgtggaa ttattatatt tatctccgac
     1321 gatattctca tcagtgaaat ccaggggaat tctcatgttt gacagcttat catcgataag
     1381 ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa
     1441 tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc
     1501 ttggttatgc cggtactgcc gggcctcttg cgggataact gcacattcgg gatatttctc
     1561 tatattcgcg cttcatcaga aaactgaagg aacctccatt gaatcgaact aatatttttt
     1621 ttggtgaatc gcattctgac tggttgcctg tcagaggcgg agaatctggt gattttgttt
     1681 ttcgacgtgg tgacgggcat gccttcgcga aaatcgcacc tgcttcccgc cgcggtgagc
     1741 tcgctggaga gcgtgaccgc ctcatttggc tcaaaggtcg aggtgtggct tgccccgagg
     1801 tcatcaactg gcaggaggaa caggagggtg catgcttggt gataacggca attccgggag
     1861 taccggcggc tgatctgtct ggagcggatt tgctcaaagc gtggccgtca atggggcagc
     1921 aacttggcgc tgttcacagc ctatcggttg atcaatgtcc gtttgagcgc aggctgtcgc
     1981 gaatgttcgg acgcgccgtt gatgtggtgt cccgcaatgc cgtcaatccc gacttcttac
     2041 cggacgagga caagagtacg ccgctgcacg atcttttggc tcgtgtcgaa cgagagctac
     2101 cggtgcggct cgaccaagag cgcaccgata tggttgtttg ccatggtgat ccctgcatgc
     2161 cgaacttcat ggtggaccct aaaactcttc aatgcacggg tctgatcgac cttgggcggc
     2221 tcggaacagc agatcgctat gccgatttgg cactcatgat tgctaacgcc gaagagaact
     2281 gggcagcgcc agatgaagca gagcgcgcct tcgctgtcct attcaatgta ttggggatcg
     2341 aagcccccga ccgcgaacgc cttgccttct atctgcgatt ggaccctctg acttggggtt
     2401 gatgttcatg ccgcctgttt ttcctgctca ttggcacgtt tcgcaacctg ttctcattgc
     2461 ggacaccttt tccagcctcg tttggaaagt ttcattgcca gacgggactc ctgcaatcgt
     2521 caagggattg aaacctatag aagacattgc tgatgaactg cgcggggccg actatctggt
     2581 atggcgcaat gggaggggag cagtccggtt gctcggtcgt gagaacaatc tgatgttgct
     2641 cgaatatgcc ggggagcgaa tgctctctca catcgttgcc gagcacggcg actaccaggc
     2701 gaccgaaatt gcagcggaac taatggcgaa gctgtatgcc gcatctgagg aacccctgcc
     2761 ttctgccctt ctcccgatcc gggatcgctt tgcagctttg tttcagcggg cgcgcgatga
     2821 tcaaaacgca ggttgtcaaa ctgactacgt ccacgcggcg attatagccg atcaaatgat
     2881 gagcaatgcc tcggaactgc gtgggctaca tggcgatctg catcatgaaa acatcatgtt
     2941 ctccagtcgc ggctggctgg tgatagatcc cgtcggtctg gtcggtgaag tgggctttgg
     3001 cgccgccaat atgttctacg atccggctga cagagacgac ctttgtctcg atcctagacg
     3061 cattgcacag atggcggacg cattctctcg tgcgctggac gtcgatccgc gtcgcctgct
     3121 cgaccaggcg tacgcttatg ggtgcctttc cgcagcttgg aacgcggatg gagaagagga
     3181 gcaacgcgat ctagctatcg cggccgcgat caagcaggtg cgacagacgt catactagat
     3241 atctagatcc ggcccgatgc gtccggcgta gaggatctga agatcagcag ttcaacctgt
     3301 tgatagtacg tactaagctc tcatgtttca cgtactaagc tctcatgttt aacgtactaa
     3361 gctctcatgt ttaacgaact aaaccctcat ggctaacgta ctaagctctc atggctaacg
     3421 tactaagctc tcatgtttca cgtactaagc tctcatgttt gaacaataaa attaatataa
     3481 atcagcaact taaatagcct ctaaggtttt aagttttata agaaaaaaaa gaatatataa
     3541 ggcttttaaa gcttttaagg tttaacggtt gtggacaaca agccagggat gtaacgcact
     3601 gagaagccct tagagcctct caaagcaatt ttcagtgaca caggaacact taacggctga
     3661 catgggaatt ctactgtttg ggtgtatgag ctatttgcaa aggaaattgg tgatgacaaa
     3721 gctcggcgct atttgaagaa aatcgactat ggcaacgccg atcctttttg tccggtgttg
     3781 ggttgaaggt gaagccggtc ggggccgcag cgggggccgg cttttcagcc ttgcccccct
     3841 gcttcggccg ccgtggctcc ggcgtcttgg gtgccggcgc gggttccgca gccttggcct
     3901 gcggtgcggg cacatcggcg ggcttggcct tgatgtgccg cctggcgtgc gagcggaacg
     3961 tctcgtagga gaacttgacc ttccccgttt cccgcatgtg ctcccaaatg gtgacgagcg
     4021 catagccgga cgctaacgcc gcctcgacat ccgccctcac cgccaggaac gcaaccgcag
     4081 cctcatcacg ccggcgcttc ttggccgcgc gggattcaac ccactcggcc agctcgtcgg
     4141 tgtagctctt tggcatcgtc tctcgcctgt cccctcagtt cagtaatttc ctgcatttgc
     4201 ctgtttccag tcggtagata ttccacaaaa cagcagggaa gcagcgcttt tccgctgcat
     4261 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat
     4321 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc
     4381 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt
     4441 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gctaccgccg
     4501 gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gccaaccagg aagggcagcc
     4561 cacctatcaa ggtgtactgc cttccagacg aacgaagagc gattgaggaa aaggcggcgg
     4621 cggccggcat gagcctgtcg gcctacctgc tggccgtcgg ccagggctac aaaatcacgg
     4681 gcgtcgtgga ctatgagcac gtccgcgagc tggcccgcat caatggcgac ctgggccgcc
     4741 tgggcggcct gctgaaactc tggctcaccg acgacccgcg cacggcgcgg ttcggtgatg
     4801 ccacgatccg ggcaacgttg ttgccattgc tgcagttcac ttacaccgct tctcaacccg
     4861 gtacgcacca taaaatcatt gatatggcca tgaatggcgt tggatgccgg gcaacagccc
     4921 gcattatggg cgttggcctc aacacgattt tacgtcactt aaaaaactca ggccgcagtc
     4981 ggtaacctcg cgcatacagc cgggcagtga cgtcatcgtc tgcgcggaaa tggacgaaca
     5041 gtggggctat gtcggggcta aatcgcgcca gcgctggctg ttttacgcgt atgacagtct
     5101 ccggaagacg gttgttgcgc acgtattcgg tgaacgcact atggcgacgc tggggcgtct
     5161 tatgagcctg ctgtcaccct ttgacgtggt gatatggatg acggatggct ggccgctgta
     5221 tgaatcccgc ctgaagggaa agctgcacgt aatcagcaag cgatatacgc agcgaattga
     5281 gcggcataac ctgaatctga ggcagcacct ggcacggctg ggacggaagt cgctgtcgtt
     5341 ctcaaaatcg gtggagctgc atgacaaagt catcgggcat tatctgaaca taaaacacta
     5401 tcaataagtt ggagtcatta ccaaaaggtt aggaatacgg ttagccattt gcctgctttt
     5461 atatagttca tatgggattc acctttatgt tgataagaaa taaaagaaaa tgccaatagg
     5521 atatcggcat tttcttttgc gtttttattt gttaactgtt aattgtcctt gttcaaggat
     5581 gctgtctttg acaacagatg ttttcttgcc tttgatgttc agcaggaagc ttggcgcaaa
     5641 cgttgattgt ttgtctgcgt agaatcctct gtttgtcata tagcttgtaa tcacgacatt
     5701 gtttcctttc gcttgaggta cagcgaagtg tgagtaagta aaggttacat cgttaggatc
     5761 aagatccatt tttaacacaa ggccagtttt gttcagcggc ttgtatgggc cagttaaaga
     5821 attagaaaca taaccaagca tgtaaatatc gttagacgta atgccgtcaa tcgtcatttt
     5881 tgatccgcgg gagtcagtga acaggtacca tttgccgttc attttaaaga cgttcgcgcg
     5941 ttcaatttca tctgttactg tgttagatgc aatcagcggt ttcatcactt ttttcagtgt
     6001 gtaatcatcg tttagctcaa tcataccgag agcgccgttt gctaactcag ccgtgcgttt
     6061 tttatcgctt tgcagaagtt tttgactttc ttgacggaag aatgatgtgc ttttgccata
     6121 gtatgctttg ttaaataaag attcttcgcc ttggtagcca tcttcagttc cagtgtttgc
     6181 ttcaaatact aagtatttgt ggcctttatc ttctacgtag tgaggatctc tcagcgtatg
     6241 gttgtcgcct gagctgtagt tgccttcatc gatgaactgc tgtacatttt gatacgtttt
     6301 tccgtcaccg tcaaagattg atttataatc ctctacaccg ttgatgttca aagagctgtc
     6361 tgatgctgat acgttaactt gtgcagttgt cagtgtttgt ttgccgtaat gtttaccgga
     6421 gaaatcagtg tagaataaac ggatttttcc gtcagatgta aatgtggctg aacctgacca
     6481 ttcttgtgtt tggtctttta ggatagaatc atttgcatcg aatttgtcgc tgtctttaaa
     6541 gacgcggcca gcgtttttcc agctgtcaat agaagtttcg ccgacttttt gatagaacat
     6601 gtaaatcgat gtgtcatccg catttttagg atctccggct aatgcaaaga cgatgtggta
     6661 gccgtgatag tttgcgacag tgccgtcagc gttttgtaat ggccagctgt cccaaacgtc
     6721 caggcctttt gcagaagaga tatttttaat tgtggacgaa tcgaattcag gaacttgata
     6781 tttttcattt ttttgctgtt cagggatttg cagcatatca tggcgtgtaa tatgggaaat
     6841 gccgtatgtt tccttatatg gcttttggtt cgtttctttc gcaaacgctt gagttgcgcc
     6901 tcctgccagc agtgcggtag taaaggttaa tactgttgct tgttttgcaa actttttgat
     6961 gttcatcgtt catgtctcct tttttatgta ctgtgttagc ggtctgcttc ttccagccct
     7021 cctgtttgaa gatggcaagt tagttacgca caataaaaaa agacctaaaa tatgtaaggg
     7081 gtgacgccaa agtatacact ttgcccttta cacattttag gtcttgcctg ctttatcagt
     7141 aacaaacccg cgcgatttac ttttcgacct cattctatta gactctcgtt tggattgcaa
     7201 ctggtctatt ttcctctttt gtttgataga aaatcataaa aggatttgca gactacgggc
     7261 ctaaagaact aaaaaatcta tctgtttctt ttcattctct gtatttttta tagtttctgt
     7321 tgcatgggca taaagttgcc tttttaatca caattcagaa aatatcataa tatctcattt
     7381 cactaaataa tagtgaacgg caggtatatg tgatgggtta aaaaggatct taaggcctag
     7441 gtctagcttg gctgcagggg gggggggggg acttccattg ttcattccac ggacaaaaac
     7501 agagaaagga aacgacagag gccaaaaagc ctcgctttca gcacctgtcg tttcctttct
     7561 tttcagaggg tattttaaat aaaaacatta agttatgacg aagaagaacg gaaacgcctt
     7621 aaaccggaaa attttcataa atagcgaaaa cccgcgaggt cgccgccccg taacctgtcg
     7681 gatcaccgga aaggacccgt aaagtgataa tgattatcat ctacatatca caacgtgcgt
     7741 ggaggccatc aaaccacgtc aaataatcaa ttatgacgca ggtatcgtat taattgatct
     7801 gcatcaactt aacgtaaaaa caacttcaga caatacaaat cagcgacact gaatacgggg
     7861 caacctcatg tccccccccc cccctgcag
//
