LOCUS       pLenti6-Flag-puro        8811 bp    DNA     circular SYN 23-NOV-2015
DEFINITION  LMBP 9166.
ACCESSION   pLenti6/VSV-DEST
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9166
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 23-NOV-2015

COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|751296766|
COMMENT     VNTDBDATE|751296766|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLenti6-Flag puro|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pLenti6-Flag puro" 0 8811 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 0 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
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COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 7685 7708 1 8811 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
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COMMENT        #2=(CFSignal (CObList) "attR2" 0 0 0 7517 7641 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "7517..7641") 
COMMENT        #3=(CFSignal (CObList) "SV40 early promoter" 29 0 0 7744 8026 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "8482..8764") 
COMMENT        #4=(CFSignal (CObList) "SV40 ori" 33 0 0 7983 8058 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "8721..8796") 
COMMENT        #5=(CFSignal (CObList) "SV40 polyA early" 43 0 0 323 397 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "323..397") 
COMMENT        #6=(CFSignal (CObList) "RSV/HIV-1 5' LTR" 29 0 0 3579 3907 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3579..3907") 
COMMENT        #7=(CFSignal (CObList) "f1 origin" 33 0 0 826 1281 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "826..1281") 
COMMENT        #8=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 5304 5820 
COMMENT            0 (CStringList) (CStringList) 1 1 1 1 "5304..5820") 
COMMENT        #9=(CFSignal (CObList) "ampicillin resistance" 4 0 0 1412 2272 0 
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COMMENT        #10=(CFSignal (CObList) "attR1" 0 0 1 5937 6061 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 0 2813 3215 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "lac promoter" 30 0 0 3307 3390 0 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "T3 promoter" 30 0 0 3454 3470 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3454..3470") 
COMMENT        #14=(CFSignal (CObList) "SV40 polyA late" 43 0 1 323 397 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "SV40 ori" 33 0 0 533 608 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "T7 promoter" 30 0 1 642 658 0 (CStringList) 
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COMMENT        #18=(CFSignal (CObList) "HIV-1 Rev response element" 21 0 0 4572 4805 0 
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COMMENT        #19=(CFSignal (CObList) "RSV/HIV-1 dU3/3' LTR" 43 0 0 17 251 0 
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COMMENT        #21=(CFSignal (CObList) "puromycin resistance" 4 0 0 8135 8737 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "8873..9475") 
COMMENT        #22=(CFSignal (CObList) "FLAG epitope" 22 0 0 7685 7708 0 (CStringList) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 11/23/15 01:32" 1) 
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COMMENT                          "length: 8811 bp" 1) 
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COMMENT                          "Division: SYN" 1) 
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COMMENT                          "Modification Date in the Original DB: 20-NOV-2015" 1) 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/pLenti6/VSV-DEST\">pLenti6/VSV-DEST</a>" 
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FEATURES             Location/Qualifiers
     CDS             6170..6829
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             7171..7476
                     /vntifkey="4"
                     /label=ccdB
     attenuator      7517..7641
                     /vntifkey="0"
                     /label=attR2
     promoter        7744..8026
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      7983..8058
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      323..397
                     /vntifkey="43"
                     /label=SV40\polyA\early
     promoter        3579..3907
                     /vntifkey="29"
                     /label=RSV/HIV-1\5'\LTR
     rep_origin      826..1281
                     /vntifkey="33"
                     /label=f1\origin
     promoter        5304..5820
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     CDS             1412..2272
                     /vntifkey="4"
                     /label=ampicillin\resistance
     attenuator      complement(5937..6061)
                     /vntifkey="0"
                     /label=attR1
     rep_origin      2813..3215
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        3307..3390
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        3454..3470
                     /vntifkey="30"
                     /label=T3\promoter
     terminator      complement(323..397)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      533..608
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(642..658)
                     /vntifkey="30"
                     /label=T7\promoter
     misc_feature    4018..4062
                     /vntifkey="21"
                     /label=HIV-1\psi\packaging\signal
     misc_feature    4572..4805
                     /vntifkey="21"
                     /label=HIV-1\Rev\response\element
     terminator      17..251
                     /vntifkey="43"
                     /label=RSV/HIV-1\dU3/3'\LTR
     misc_feature    1..16
                     /vntifkey="21"
                     /label=HIV-1\cPPT
     CDS             8135..8737
                     /vntifkey="4"
                     /label=puromycin\resistance
     misc_marker     7685..7708
                     /vntifkey="22"
                     /label=FLAG\epitope
BASE COUNT     2308 a      2103 c      2237 g      2163 t 
ORIGIN
        1 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt 
       61 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 
      121 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 
      181 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 
      241 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 
      301 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 
      361 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 
      421 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg 
      481 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 
      541 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 
      601 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg 
      661 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 
      721 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca 
      781 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg 
      841 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 
      901 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 
      961 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 
     1021 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 
     1081 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 
     1141 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 
     1201 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 
     1261 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat 
     1321 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 
     1381 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 
     1441 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 
     1501 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 
     1561 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 
     1621 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 
     1681 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 
     1741 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 
     1801 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 
     1861 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 
     1921 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 
     1981 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 
     2041 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 
     2101 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 
     2161 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 
     2221 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 
     2281 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 
     2341 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 
     2401 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 
     2461 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 
     2521 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 
     2581 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 
     2641 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 
     2701 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 
     2761 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 
     2821 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 
     2881 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 
     2941 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 
     3001 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 
     3061 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 
     3121 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 
     3181 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 
     3241 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 
     3301 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 
     3361 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 
     3421 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa 
     3481 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta 
     3541 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg 
     3601 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt 
     3661 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac 
     3721 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 
     3781 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 
     3841 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 
     3901 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc 
     3961 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga 
     4021 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca 
     4081 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa 
     4141 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag 
     4201 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac 
     4261 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct 
     4321 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg 
     4381 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga 
     4441 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt 
     4501 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga 
     4561 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc 
     4621 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag 
     4681 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg 
     4741 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag 
     4801 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat 
     4861 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg 
     4921 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac 
     4981 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat 
     5041 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc 
     5101 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga 
     5161 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa 
     5221 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga 
     5281 tctcgacggt atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg 
     5341 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 
     5401 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 
     5461 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 
     5521 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 
     5581 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 
     5641 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 
     5701 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 
     5761 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 
     5821 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga 
     5881 agacaccgac tctagaggat ccactagtcc agtgtggtgg aattctgcag atatcaacaa 
     5941 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt 
     6001 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat 
     6061 ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg 
     6121 gattttgagt taggatccgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa 
     6181 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc 
     6241 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt 
     6301 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc 
     6361 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat 
     6421 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc 
     6481 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt 
     6541 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt 
     6601 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga 
     6661 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct 
     6721 gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat 
     6781 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa gatctggatc 
     6841 cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag 
     6901 aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtgtgctat gaagcagcgt 
     6961 attacagtga cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata 
     7021 tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct 
     7081 ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct 
     7141 cttttgctga cgagaacagg gactggtgaa atgcagttta aggtttacac ctataaaaga 
     7201 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga 
     7261 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt 
     7321 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt 
     7381 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc 
     7441 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccgt tatacacagc 
     7501 cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg 
     7561 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc 
     7621 agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtctagaggg 
     7681 cccggactac aaggatgacg atgacaaaac gcgtaccggt tagtaatgag tttggaatta 
     7741 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag 
     7801 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 
     7861 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 
     7921 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 
     7981 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 
     8041 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 
     8101 tatatccatt ttcggatctg atcagcacgt gaccatggcc accgagtaca agcccacggt 
     8161 gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc 
     8221 cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga 
     8281 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga 
     8341 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt 
     8401 cgccgagatc ggctcgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca 
     8461 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt 
     8521 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt 
     8581 ggaggcggcc gagcgcgctg gggtgcccgc cttcctggag acctccgcgc cccgcaacct 
     8641 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc 
     8701 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagct aagcacaatt cgagctcggt 
     8761 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt t 
//
