LOCUS       pLoxGentrc              6295 bp    DNA     circular     19-JUN-2014
DEFINITION  LMBP 9151.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9151
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 19-JUN-2014
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|697210922|
COMMENT     VNTDBDATE|701170734|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLoxGentrc|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     rep_origin      complement(1..456)
                     /vntifkey="33"
                     /label=M13\ori
     misc_feature    complement(763..796)
                     /vntifkey="21"
                     /label=loxP
     misc_feature    complement(1599..1632)
                     /vntifkey="21"
                     /label=loxP
     terminator      complement(1716..1743)
                     /vntifkey="43"
                     /label=T2\terminator
     terminator      1875..1918
                     /vntifkey="43"
                     /label=T1\terminator
     RBS             complement(2185..2222)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        complement(2223..2266)
                     /vntifkey="30"
                     /label=trc\promoter
     CDS             complement(2489..3571)
                     /vntifkey="4"
                     /label=lacI
     promoter        complement(3572..3649)
                     /vntifkey="30"
                     /label=lacIq\promoter,\incl.\RBS
     RBS             complement(3893..3930)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        complement(3931..4014)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(4106..4508)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(5049..5909)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             complement(840..1373)
                     /vntifkey="4"
                     /label=gentamicin\resistance
BASE COUNT     1530 a      1639 c      1634 g      1492 t 
ORIGIN
        1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat 
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagcccgaga 
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca 
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccca 
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc 
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag 
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca 
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtacta tggttgcttt gacgtatgcg 
      481 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 
      541 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 
      601 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 
      661 acgacgttgt aaaacgacgg ccagtgccaa gctagcttaa ggtgcacggc ccacgtggcc 
      721 actagtactt ctcgaggtcg acggtatcga taagctggat ccataacttc gtataatgta 
      781 tgctatacga agttatctag cgcgtcggcc gggaagccga tctcggcttg aacgaattgt 
      841 taggtggcgg tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt 
      901 gtatagagag ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca 
      961 ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg cggtggcaat gccctgcctc 
     1021 cggtgctcgc cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac 
     1081 ctgggcagaa cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc 
     1141 gcgatgaatg tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg 
     1201 gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat 
     1261 ttgacttggt cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac 
     1321 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa catcgttgct 
     1381 gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg 
     1441 catagactgt acaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc 
     1501 accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta 
     1561 cagtttacga accgaacagg cttatgtcga cgcgctagat aacttcgtat aatgtatgct 
     1621 atacgaagtt atgcggccgc gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
     1681 tagaaaaata aacaaaaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct 
     1741 tctgcttaat ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc 
     1801 gttgcttcgc aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca 
     1861 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 
     1921 gatgcctggc agttccctac tctcgcatgg ggagacccca cactaccatc ggcgctacgg 
     1981 cgtttcactt ctgagttcgg catggggtca ggtgggacca ccgcgctact gccgccaggc 
     2041 aaattctgtt ttatcagacc gcttctgcgt tctgatttaa tctgtatcag gctgaaaatc 
     2101 ttctctcatc cgccaaaaca gccaagcttg catgcctgca ggtcgactct agaggatccc 
     2161 cgggtaccga gctcgaattc catggtctgt ttcctgtgtg aaattgttat ccgctcacaa 
     2221 ttccacacat tatacgagcc ggatgattaa ttgtcaacag ctcatttcag aatatttgcc 
     2281 agaaccgtta tgatgtcggc gcaaaaaaca ttatccagaa cgggagtgcg ccttgagcga 
     2341 cacgaattat gcagtgattt acgacctgca cagccatacc acagcttccg atggctgcct 
     2401 gacgccagaa gcattggtgc accgtgcagt cgatgataag ctgtcaaacc agatcaattc 
     2461 gcgctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 
     2521 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 
     2581 ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct 
     2641 ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct 
     2701 gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca 
     2761 ctaccgagat atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca 
     2821 gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt 
     2881 gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct 
     2941 gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag 
     3001 aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca 
     3061 cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag 
     3121 agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct 
     3181 ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca 
     3241 ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac 
     3301 ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca 
     3361 gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc 
     3421 ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag 
     3481 aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact 
     3541 ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg 
     3601 ggcgctatca tgccataccg cgaaaggttt tgcaccattc gatggtgtca acgtaaatgc 
     3661 atgccgcttc gccttcgcgc gcgaattgat ctgctgcctc gcgcgtttcg gtgatgacgg 
     3721 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 
     3781 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc 
     3841 catgacccag tcacgtagcg atagcggagt gtaaattcgt aatcatggtc atagctgttt 
     3901 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 
     3961 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 
     4021 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 
     4081 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 
     4141 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 
     4201 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 
     4261 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 
     4321 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 
     4381 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 
     4441 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 
     4501 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 
     4561 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 
     4621 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 
     4681 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 
     4741 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 
     4801 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 
     4861 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 
     4921 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 
     4981 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 
     5041 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 
     5101 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 
     5161 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 
     5221 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 
     5281 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 
     5341 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 
     5401 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 
     5461 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 
     5521 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 
     5581 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 
     5641 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 
     5701 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 
     5761 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 
     5821 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 
     5881 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 
     5941 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 
     6001 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt 
     6061 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc 
     6121 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg 
     6181 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt 
     6241 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccata 
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