LOCUS       pLoxGentrcmelA          8108 bp    DNA     circular     13-JUN-2014
DEFINITION  LMBP 9150.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9150
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 13-JUN-2014
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|697217533|
COMMENT     VNTDBDATE|700673575|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLoxGentrcmelA|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     rep_origin      complement(1..456)
                     /vntifkey="33"
                     /label=M13\ori
     misc_feature    complement(763..796)
                     /vntifkey="21"
                     /label=loxP
     misc_feature    complement(1599..1632)
                     /vntifkey="21"
                     /label=loxP
     terminator      complement(1716..1743)
                     /vntifkey="43"
                     /label=T2\terminator
     terminator      1875..1918
                     /vntifkey="43"
                     /label=T1\terminator
     RBS             complement(3998..4035)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        complement(4036..4079)
                     /vntifkey="30"
                     /label=trc\promoter
     CDS             complement(4302..5384)
                     /vntifkey="4"
                     /label=lacI
     promoter        complement(5385..5462)
                     /vntifkey="30"
                     /label=lacIq\promoter,\incl.\RBS
     RBS             complement(5706..5743)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        complement(5744..5827)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(5919..6321)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(6862..7722)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             complement(2167..3996)
                     /vntifkey="4"
                     /label=melA
     CDS             complement(840..1373)
                     /vntifkey="4"
                     /label=gentamicin\resistance
BASE COUNT     1877 a      2131 c      2201 g      1899 t 
ORIGIN
        1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat 
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagcccgaga 
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca 
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccca 
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc 
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag 
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca 
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtacta tggttgcttt gacgtatgcg 
      481 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 
      541 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 
      601 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 
      661 acgacgttgt aaaacgacgg ccagtgccaa gctagcttaa ggtgcacggc ccacgtggcc 
      721 actagtactt ctcgaggtcg acggtatcga taagctggat ccataacttc gtataatgta 
      781 tgctatacga agttatctag cgcgtcggcc gggaagccga tctcggcttg aacgaattgt 
      841 taggtggcgg tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt 
      901 gtatagagag ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca 
      961 ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg cggtggcaat gccctgcctc 
     1021 cggtgctcgc cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac 
     1081 ctgggcagaa cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc 
     1141 gcgatgaatg tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg 
     1201 gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat 
     1261 ttgacttggt cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac 
     1321 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa catcgttgct 
     1381 gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg 
     1441 catagactgt acaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc 
     1501 accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta 
     1561 cagtttacga accgaacagg cttatgtcga cgcgctagat aacttcgtat aatgtatgct 
     1621 atacgaagtt atgcggccgc gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
     1681 tagaaaaata aacaaaaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct 
     1741 tctgcttaat ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc 
     1801 gttgcttcgc aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca 
     1861 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 
     1921 gatgcctggc agttccctac tctcgcatgg ggagacccca cactaccatc ggcgctacgg 
     1981 cgtttcactt ctgagttcgg catggggtca ggtgggacca ccgcgctact gccgccaggc 
     2041 aaattctgtt ttatcagacc gcttctgcgt tctgatttaa tctgtatcag gctgaaaatc 
     2101 ttctctcatc cgccaaaaca gccaagcttg catgcctgca ggtcgactct agaggatccc 
     2161 cgggtgttag gcggacacta tggctatttc tagctttgcg ggctctacgg cgccgggttt 
     2221 gcccgctccc gatccgacag gaatgagctg caggtcgatg gcctcgccat ccgtctgccc 
     2281 ccttccgccg taaacccgct ggatcgcgtc cgtcagatcg aggacgaagg atggtttgcg 
     2341 atgatggtcg ccgtcatgac cgagaacggc gaaagaaccg acgtaatggg gatcggtgac 
     2401 gggcgtatcg gccttaaggt cgttcttgcc gagaaacacc cgaacgctgg tcgtgctggc 
     2461 actggtgatc tcgacatcgc gcaggaatgc cagcacacga ggagcggagg ccgcctgctc 
     2521 ctgtgccgcg ccgaaatcca ttgtatccat gccggatggc agaggcggtt ggcggacgat 
     2581 ctcctgcaat gcgcccgccg ggatcttgat cggcagcgaa agcggcacgt tttccgttgc 
     2641 cgccttgctg ttgtccgtgg aggtggtcgt cacgccggcg attgcagcat cggtcgccgt 
     2701 cgccgcgatc acggacgtga gtttatcgtt cagggccagc gttttggcgc tcgccgccgc 
     2761 gaccttgaag taggtccgga aaccataatt gtatccgagt tcctctggaa cataaaggtc 
     2821 agagaccttc ggggaccaga agttgccgtc gacatcgtag aaattgtcgg tgaacggcat 
     2881 gtcggcccag agtcgatccg tgctgttggc gttgcgcaaa ttccaggtcg cccagatgcg 
     2941 gtcgatgttg caatgatgca tgaagaagat cggatcgcgg ggcgacgaca tttccggcat 
     3001 ccagccaccg atattgttgt gcacctgatt gtgcggtgtg tattccagcg ccccctgcgt 
     3061 gccgctgctg gtggtgaccc aggaaggatc gagtgagttc tgtccctcgg ggcgggtggt 
     3121 gccgaagacc tcgtatggct tcgccgttag gatggtgttg agaacctgtg gcccaactat 
     3181 attgtccggc atcggctgcg tgattggcca ggtgcgcgac gaaacataaa gtggattgtc 
     3241 cttgccgtcg ggcgtctttt gcattgtgaa cacttcgggc aggtacggat tgtcggtcca 
     3301 gtcccagaac ggcatagcga aatcgttgtt cttggtcacg tgccgaacga tgcgctcgta 
     3361 catagccgta taggccctgt gccacggcag gaaataccag ttgccatgcg ggcagtattt 
     3421 gacgacgtca ccgctgccgt gaattttcga gaggttgacc cagttgaatt tgtcgctggc 
     3481 gggaagggcg ttgagaaggc gcactgcgtc gcgataggtc tcgatgatcg ggtcgttcca 
     3541 ggccaagccc tgcagtgagc gccgccaagg gattgacggt atctgcgcga aggcttttgt 
     3601 ccccggtagg ccgctggcga gcaggcctgc tgcaatgacg ccaccctgaa cgatgacatg 
     3661 tctgcgtgtg atgctcatct tactctccct tggatttgaa ttgtccttcg ccaagattaa 
     3721 tgccgctttt aggccggttc cgcgccgtct gggcgcggga gcagtgggca attcgcacca 
     3781 ccatagagct tgcgttggct ctttctgagt ttcggtgaat cggcggcacg agaaatgaag 
     3841 caagcgacgt gccactcgaa aatgcagcgt aaaatgcgag ctaaaaacgc tactaccgat 
     3901 cgtattcctg cattccagtt ggaatccaga ttccggaaaa cttagtatcg cattccatga 
     3961 tcgttcgagc gacggcttgc cgaccagcca cgccatggtc tgtttcctgt gtgaaattgt 
     4021 tatccgctca caattccaca cattatacga gccggatgat taattgtcaa cagctcattt 
     4081 cagaatattt gccagaaccg ttatgatgtc ggcgcaaaaa acattatcca gaacgggagt 
     4141 gcgccttgag cgacacgaat tatgcagtga tttacgacct gcacagccat accacagctt 
     4201 ccgatggctg cctgacgcca gaagcattgg tgcaccgtgc agtcgatgat aagctgtcaa 
     4261 accagatcaa ttcgcgctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 
     4321 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 
     4381 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg 
     4441 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc 
     4501 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta 
     4561 tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg 
     4621 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc 
     4681 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt 
     4741 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga 
     4801 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg 
     4861 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg 
     4921 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca 
     4981 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg 
     5041 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc 
     5101 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc 
     5161 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt 
     5221 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc 
     5281 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag 
     5341 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat 
     5401 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcacca ttcgatggtg 
     5461 tcaacgtaaa tgcatgccgc ttcgccttcg cgcgcgaatt gatctgctgc ctcgcgcgtt 
     5521 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 
     5581 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 
     5641 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtaaatt cgtaatcatg 
     5701 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 
     5761 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 
     5821 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 
     5881 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 
     5941 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 
     6001 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 
     6061 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 
     6121 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 
     6181 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 
     6241 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 
     6301 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 
     6361 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
     6421 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 
     6481 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 
     6541 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 
     6601 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 
     6661 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 
     6721 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 
     6781 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 
     6841 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 
     6901 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 
     6961 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 
     7021 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 
     7081 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 
     7141 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 
     7201 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 
     7261 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 
     7321 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 
     7381 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 
     7441 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 
     7501 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 
     7561 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 
     7621 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 
     7681 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 
     7741 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 
     7801 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 
     7861 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 
     7921 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 
     7981 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 
     8041 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 
     8101 gcaccata 
//
