LOCUS       pPGK-loxP-frt-neo-frt 6985 bp dsDNACircular
DEFINITION  LMBP 8981.
COMMENT     This file is created by Vector NTI 4.0 from InforMax, Inc., USA:
COMMENT     (800)357-3114 or http://www.informaxinc.com/
FEATURES               Location/Qualifiers
     misc_feature    6279..6312
                     /label=FRT\site
     misc_feature    6833..6957
                     /label=Tn5\Promoter
     misc_feature    1072..1105
                     /label=FRT\site
     misc_feature    6167..6200
                     /label=loxP
     CDS             1..795
                     /label=NEO
     CDS             1357..2214
                     /note="ORF: Frame #1   Start: atg   Stop:  taa"
                     /label=Amp
ORIGIN
        1 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 
       61 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 
      121 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 
      181 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 
      241 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 
      301 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 
      361 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 
      421 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 
      481 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac 
      541 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 
      601 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 
      661 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc 
      721 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 
      781 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc 
      841 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 
      901 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 
      961 ccgtttccct gccacagtct gagagctccc tggcgaattc ggtaccaata aaagagcttt 
     1021 attttcatga tctgtgtgtt ggtttttgtg tgcggcgcgc cagcttggcg tgaagttcct 
     1081 attctctaga aagtatagga acttccgctc tcctgagtag gacaaatccg ccgggagcgg 
     1141 atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg 
     1201 ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa 
     1261 ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 
     1321 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 
     1381 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 
     1441 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 
     1501 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 
     1561 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa 
     1621 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 
     1681 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 
     1741 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 
     1801 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 
     1861 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 
     1921 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 
     1981 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 
     2041 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 
     2101 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 
     2161 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 
     2221 tcagaccaag tttactcata tatactttag attgatttac gcgccctgta gcggcgcatt 
     2281 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 
     2341 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 
     2401 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 
     2461 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 
     2521 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaacttgaac 
     2581 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc 
     2641 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt 
     2701 aacgtttaca atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 
     2761 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 
     2821 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 
     2881 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 
     2941 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 
     3001 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 
     3061 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 
     3121 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 
     3181 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 
     3241 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 
     3301 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 
     3361 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 
     3421 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 
     3481 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 
     3541 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 
     3601 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata gggtcatggc 
     3661 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc 
     3721 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc 
     3781 gtcatcaccg aaacgcgcga ggcagcaagg agatggcgcc caacagtccc ccggccacgg 
     3841 ggcctgccac catacccacg ccgaaacaag cgctcctcgt accagcgaag aaggggcaga 
     3901 gatgccgtag tcaggtttag ttcgtccggc ggcgccagaa atccgcgcgg tggtttttgg 
     3961 gggtcggggg tgtttggcag ccacagacgc ccggtgttcg tgtcgcgcca gtacatgcgg 
     4021 tccatgccca ggccatccaa aaaccatggg tctgtctgct cagtccagtc gtggacctga 
     4081 ccccacgcaa cgcccaaaat aataaccccc acgaaccata aaccattccc catgggggac 
     4141 cccgtcccta acccacgggg cccgtggcta tggcgggctt gccgccccga cgttggctgc 
     4201 gagccctggg ccttcacccg aacttggggg ttggggtggg gaaaaggaag aaacgcgggc 
     4261 gtattggccc caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac 
     4321 cccgcgttta tgaacaaacg acccaacacc cgtgcgtttt attctgtctt tttattgccg 
     4381 tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctcccccatc 
     4441 tcccgggcaa acgtgcgcgc caggtcgcag atcgtcggta tggagcctgg ggtggtgacg 
     4501 tgggtctgga ccatcccgga ggtaagttgc agcagggcgt cccggcagcc ggcgggcgat 
     4561 tggtcgtaat ccaggataaa gacgtgcatg ggacggaggc gtttggccaa gacgtccaag 
     4621 gcccaggcaa acacgttata caggtcgccg ttgggggcca gcaactcggg ggcccgaaac 
     4681 agggtaaata acgtgtcccc gatatggggt cgtgggcccg cgttgctctg gggctcggca 
     4741 ccctggggcg gcacggccgt ccccgaaagt tgtccccaat cctcccgcca cgacccgccg 
     4801 ccctgcagat accgcaccgt attggcaagc agcccgtaaa cgcggcgaat cgcggccagc 
     4861 atagccaggt caagccgctc gccggggcgc tggcgtttgg ccaggcggtc gatgtgtctg 
     4921 tcctccggaa gggcccccaa cacgatgttt gtgccgggca aggtcggcgg gatgagggcc 
     4981 acgaacgcca gcacggcctg gggggtcatg ctgcccataa ggtatcgcgc ggccgggtag 
     5041 cacaggaggg cggcgatggg atggcggtcg aagatgaggg tgagggccgg gggcggggca 
     5101 tgtgagctcc cagcctcccc cccctatatg aggagccaga acggcgtcgg tcacggcata 
     5161 aggcatgccc attgttatct gggcgcttgt cattaccacc gccgcgtccc cggccgatat 
     5221 ctcaccctgg tcgaggcggt gttgtgtggt gtagatgttc gcgattgtct cggaagcccc 
     5281 cagcacctgc cagtaagtca tcggctcggg tacgtagacg atatcgtcgc gcgaacccag 
     5341 ggccaccagc agttgcgtgg tggtggtttt ccccatcccg tgaggaccgt ctatataaac 
     5401 ccgcagtagc gtgggcattt tctgctccag gcggacttcc gtggcttctt gctgccggcg 
     5461 agggcgcaac gccgtacgtc ggttgctatg gccgcgagaa cgcgcagcct ggtcgaacgc 
     5521 agacgcgtgt tgatggcagg ggtacgaagc catacgcgct tctacaaggc gcttgccgaa 
     5581 gaggtgcggg agtttcacgc caccaagatc tgcggcacgc tgttgacgct gttaagcggg 
     5641 tcgctgcagg tcgaaaggcc cggagatgag gaagaggaga acagcgcggc agacgtgcgc 
     5701 ttttgaagcg tgcagaatgc cgggctccgg aggaccttcg ggcgcccgcc ccgcccctga 
     5761 gcccgcccct gagcccgccc ccggacccac cccttcccag cctctgagcc cagaaagcga 
     5821 aggagcaaag ctgctattgg ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg 
     5881 tgctgtccat ctgcacgaga ctagtgagac gtgctacttc catttgtcac gtcctgcacg 
     5941 acgcgagctg cggggcgggg gggaacttcc tgactagggg aggagtagaa ggtggcgcga 
     6001 aggggccacc aaagaacgga gccggttggc gcctaccggt ggatgtggaa tgtgtgcgag 
     6061 gccagaggcc acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg catgctccag 
     6121 actgccttgg gaaaagcgcc tcccctaccc ggtagaattc gtcgacataa cttcgtataa 
     6181 tgtatgctat acgaagttat ggtaccggat ccatcgatgc ataatgtgcc tgtcaaatgg 
     6241 acgaagcagg gattctgcaa accctatgct actccgtcga agttcctatt ctctagaaag 
     6301 tataggaact tcattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg 
     6361 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc tcgcacacat 
     6421 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct 
     6481 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg 
     6541 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa 
     6601 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg 
     6661 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg 
     6721 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct 
     6781 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc ggtggacagc 
     6841 aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt 
     6901 aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gatctgatca 
     6961 agagacagga tgaggatcgt ttcgc 
//

