ID pMG14 standard; circular DNA; ; 5169 BP. DT 16-OCT-2014 DE LMBP 8465. XX CC Accession number: LMBP 8465 CC (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) CC Last modified: 24-OCT-2014 CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT CDS complement(4289..5059) FT /vntifkey="4" FT /label=kanamycin/tobramycin\resistance\(aadD) FT promoter 5..234 FT /vntifkey="30" FT /label=cggR\promoter FT misc_feature complement(1010..1125) FT /vntifkey="21" FT /label=5'\UTR\of\amyE FT CDS 1334..2194 FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin 2735..3137 FT /vntifkey="33" FT /label=pMB1\ori FT promoter 3229..3312 FT /vntifkey="30" FT /label=lac\promoter FT CDS complement(3396..4199) FT /vntifkey="4" FT /label=amyE\fragment FT /note="fragment of amyE encoding amino acid 319-586" FT misc_feature 262..302 FT /vntifkey="21" FT /label=5'\UTR\of\gapA FT RBS 3313..3350 FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS FT misc_marker 311..334 FT /vntifkey="22" FT /label=Strep-tag\II FT CDS complement(470..1009) FT /vntifkey="4" FT /label=amyE\fragment FT /note="fragment of amyE encoding amino acid 1-180" SQ Sequence 5169 BP; 1379 A; 1209 C; 1120 G; 1461 t; aattctttgg ataaaatgaa aacgctttaa tgaaacagcc ctttctacgg gaagggctgt 60 ttatattggg atgcaccatt tggcgctttc tgtataagat aaagatatat aggataaaat 120 attgctggat aaaacgacgc ggcatgaaaa ctctgcgaat attgtcgatg aattggctct 180 taacagttga ataaacaatt ccaccctgtt aaaataatta aagaaagcag aaatctgcag 240 aagttattaa gggatgaata atccctcaat ataaatatct ctcacttatt taaaggagga 300 aagcttcatg tggagccacc cgcagttcga aaaaggtacc cggggatcct ctagagtcct 360 gagcgccggt cgctaccatt accagttggt ctggtgtcaa aaataataat aaccgggcag 420 gccatgtctg cccgtatttc gcgtaaggaa atccattatg tactatttaa ttctgcgtga 480 catcccatcg atcagaccag tttttaattt gtgtgtttcc atgtgtccag tttggaatac 540 tcttaacctc attggaaatc gcggcataat cactggtggt atgattgatg accgcgtcaa 600 caatgacctt tatgccatat tcttcagcgg ctgcacacat ttctttaaat tcttgttcag 660 tacctaagta acggttgcca atttgatacg atgtcggctg atacagccag taccagttcg 720 acatgctttt atctccttga ttcccttcct ttacttggtt aatcggagat gtctgaatgg 780 ctgtatatcc tgcatcatga atatccttca tattgtgttt taacgtattg aacgaccaat 840 tccatgcatg aagaatggtt ccgcttttga tcgacggtgc tgtaagctca ttcgatttgt 900 tcgccgtttc agcactcgca gccgccggtc ctgccagaac caaatgaaac agcaataaaa 960 atccagcgaa taacggcagt aaagaggttt tgaatcgttt tgcaaacatt cttgacactc 1020 cttatttgat tttttgaaga cttacttcgg agtcaaaaat ccctcttact tcattcttcc 1080 gcttcctcct ttcaaaccga tgtgaagact ggagaatttt gttaattctt gaagacgaaa 1140 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac 1200 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 1260 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 1320 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 1380 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 1440 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 1500 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 1560 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 1620 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 1680 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 1740 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 1800 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 1860 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 1920 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 1980 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 2040 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 2100 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 2160 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 2220 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 2280 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 2340 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 2400 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 2460 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 2520 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 2580 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 2640 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 2700 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 2760 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 2820 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 2880 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 2940 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 3000 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 3060 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag 3120 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 3180 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 3240 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 3300 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga 3360 ttacgccaag ctaatcggcg gccgcatcga tgcaggctcc catcatgttt atagatataa 3420 gcatttacct ggctccaatg attcggattt tgatagccga tggttttggc cgacgctgga 3480 tctcttttaa caaaactgta tttctcggtc ctcgttacac catcactgtt cgttcctttt 3540 aacatgatgg tgtatgtttt gccaaatgga tctccttttc cgattgtgaa ttgatctcca 3600 tccttaaacg ccgtctctgg tccattattg atttgataaa cggcttttgt tgtattcgca 3660 tctgcacgca aggtaatcgt cagttgatca ttgaaagaat gtgttacacc tgttttgtaa 3720 ttctcaagga aaacatgagg cgcttttgca atatcatcag gataaagcac agctacagac 3780 ctggcattga tcgtgcctgt cagtttacca tcgttcactt gaaatgaacc cgctccagct 3840 ttattgtcat acctgccatc aggcaatttt gttgccgtat tgatagagac agaggatgaa 3900 cctgcatttg ccagcacaac gccatgtgag ccgcgctgat tcataaatat ctggttgttt 3960 ccattcgggt tcgagagttc ctcaggctgt ccagccatca cattgtgaaa tctattgacc 4020 gcagtgatag cctgatcttc aaataaagca ctcccgcgat cgcctatttg gcttttcccc 4080 gggaacctca caccatttcc gcctccctca ggtctggaaa agaaaagagg cgtactgcct 4140 gaacgagaag ctatcaccgc ccagcctaaa cggatatcat catcgctcat ccatgtcgac 4200 gcgccatgac agccatgata attactaata ctaggagaag ttaataaata cgtaaccaac 4260 atgattaaca attattagag gtcatcgttc aaaatggtat gcgttttgac acatccacta 4320 tatatccgtg tcgttctgtc cactcctgaa tcccattcca gaaattctct agcgattcca 4380 gaagtttctc agagtcggaa agttgaccag acattacgaa ctggcacaga tggtcataac 4440 ctgaaggaag atctgattgc ttaactgctt cagttaagac cgaagcgctc gtcgtataac 4500 agatgcgatg atgcagacca atcaacatgg cacctgccat tgctacctgt acagtcaagg 4560 atggtagaaa tgttgtcggt ccttgcacac gaatattacg ccatttgcct gcatattcaa 4620 acagctcttc tacgataagg gcacaaatcg catcgtggaa cgtttgggct tctaccgatt 4680 tagcagtttg atacactttc tctaagtatc cacctgaatc ataaatcggc aaaatagaga 4740 aaaattgacc atgtgtaagc ggccaatctg attccacctg agatgcataa tctagtagaa 4800 tctcttcgct atcaaaattc acttccacct tccactcacc ggttgtccat tcatggctga 4860 actctgcttc ctctgttgac atgacacaca tcatctcaat atccgaatag ggcccatcag 4920 tctgacgacc aagagagcca taaacaccaa tagccttaac atcatcccca tatttatcca 4980 atattcgttc cttaatttca tgaacaatct tcattctttc ttctctagtc attattattg 5040 gtccattcac tattctcatt cccttttcag ataattttag atttgctttt ctaaataaga 5100 atatttggag agcaccgttc ttattcagct attaaaccca ttatatcggg tttttgaggg 5160 gatttcaag 5169 //