LOCUS       pMG12                   6119 bp    DNA     circular     21-JUN-2013
DEFINITION  LMBP 8463.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8463
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 21-JUN-2013
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|663938623|
COMMENT     VNTDBDATE|665404888|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pMG12|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #10=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 4788 0 0 "") 
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COMMENT        #17=(CFSignal (CObList) "chloramphenicol resistance" 4 0 1 5162 5812 0 
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COMMENT        #18=(CFSignal (CObList) "tetracyclin resistance" 4 0 1 72 719 0 
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FEATURES             Location/Qualifiers
     misc_feature    complement(1061..1717)
                     /vntifkey="21"
                     /label=5'\UTR\of\amyE
     CDS             1926..2786
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      3327..3729
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        3821..3904
                     /vntifkey="30"
                     /label=lac\promoter
     misc_feature    complement(3987..4791)
                     /vntifkey="21"
                     /label=3'\UTR\of\amyE
     CDS             complement(5162..5812)
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             complement(72..719)
                     /vntifkey="4"
                     /label=tetracyclin\resistance
     promoter        780..825
                     /vntifkey="30"
                     /label=xyl/tet\promoter
     misc_feature    831..926
                     /vntifkey="21"
                     /label=5'\UTR\of\gapA
BASE COUNT     1662 a      1372 c      1297 g      1788 t 
ORIGIN
        1 gaattcatcg ataactcgac atcttggtta ccgtgaagtt accatcacgg aaaaaggtta 
       61 tgctgctttt aagacccact ttcacattta agttgttttt ctaatccgca tatgatcaat 
      121 tcaaggccga ataagaaggc tggctctgca ccttggtgat caaataattc gatagcttgt 
      181 cgtaataatg gcggcatact atcagtagta ggtgtttccc tttcttcttt agcgacttga 
      241 tgctcttgat cttccaatac gcaacctaaa gtaaaatgcc ccacagcgct gagtgcatat 
      301 aatgcattct ctagtgaaaa accttgttgg cataaaaagg ctaattgatt ttcgagagtt 
      361 tcatactgtt tttctgtagg ccgtgtacct aaatgtactt ttgctccatc gcgatgactt 
      421 agtaaagcac atctaaaact tttagcgtta ttacgtaaaa aatcttgcca gctttcccct 
      481 tctaaagggc aaaagtgagt atggtgccta tctaacatct caatggctaa ggcgtcgagc 
      541 aaagcccgct tattttttac atgccaatac aatgtaggct gctctacacc tagcttctgg 
      601 gcgagtttac gggttgttaa accttcgatt ccgacctcat taagcagctc taatgcgctg 
      661 ttaatcactt tacttttatc taatctagac atcattaatt cctccttttt ggttgacact 
      721 ctatcattga tagagttatt tgtcaaacta gttttttatt tggatcgttc atgaaaaact 
      781 aaaaaaaata ttgacactct atcattgata gagtataatt aaaatactgc agaagttatt 
      841 aagggatgaa taatccctca atataaatat ctctcactta tttaaaggag gaaagcttca 
      901 tgtggagcca cccgcagttc gaaaaaggta cccggggatc ctctagagtc ctgagcgccg 
      961 gtcgctacca ttaccagttg gtctggtgtc aaaaataata ataaccgggc aggccatgtc 
     1021 tgcccgtatt tcgcgtaagg aaatccatta tgtactattt aattctgcgt gacatcccat 
     1081 cgatcagacc agtttttaat ttgtgtgttt ccatgtgtcc agtttggaat actcttaacc 
     1141 tcattggaaa tcgcggcata atcactggtg gtatgattga tgaccgcgtc aacaatgacc 
     1201 tttatgccat attcttcagc ggctgcacac atttctttaa attcttgttc agtacctaag 
     1261 taacggttgc caatttgata cgatgtcggc tgatacagcc agtaccagtt cgacatgctt 
     1321 ttatctcctt gattcccttc ctttacttgg ttaatcggag atgtctgaat ggctgtatat 
     1381 cctgcatcat gaatatcctt catattgtgt tttaacgtat tgaacgacca attccatgca 
     1441 tgaagaatgg ttccgctttt gatcgacggt gctgtaagct cattcgattt gttcgccgtt 
     1501 tcagcactcg cagccgccgg tcctgccaga accaaatgaa acagcaataa aaatccagcg 
     1561 aataacggca gtaaagaggt tttgaatcgt tttgcaaaca ttcttgacac tccttatttg 
     1621 attttttgaa gacttacttc ggagtcaaaa atccctctta cttcattctt ccgcttcctc 
     1681 ctttcaaacc gatgtgaaga ctggagaatt ttgttaattc ttgaagacga aagggcctcg 
     1741 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 
     1801 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 
     1861 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 
     1921 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 
     1981 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 
     2041 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 
     2101 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 
     2161 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 
     2221 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 
     2281 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 
     2341 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 
     2401 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 
     2461 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 
     2521 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 
     2581 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 
     2641 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 
     2701 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 
     2761 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 
     2821 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 
     2881 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 
     2941 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 
     3001 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 
     3061 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 
     3121 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 
     3181 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 
     3241 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 
     3301 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 
     3361 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 
     3421 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 
     3481 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 
     3541 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 
     3601 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 
     3661 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 
     3721 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 
     3781 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 
     3841 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 
     3901 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 
     3961 agctaatcgg cggccgcatc gatgcaggct cccatcatgt ttatagatat aagcatttac 
     4021 ctggctccaa tgattcggat tttgatagcc gatggttttg gccgacgctg gatctctttt 
     4081 aacaaaactg tatttctcgg tcctcgttac accatcactg ttcgttcctt ttaacatgat 
     4141 ggtgtatgtt ttgccaaatg gatctccttt tccgattgtg aattgatctc catccttaaa 
     4201 cgccgtctct ggtccattat tgatttgata aacggctttt gttgtattcg catctgcacg 
     4261 caaggtaatc gtcagttgat cattgaaaga atgtgttaca cctgttttgt aattctcaag 
     4321 gaaaacatga ggcgcttttg caatatcatc aggataaagc acagctacag acctggcatt 
     4381 gatcgtgcct gtcagtttac catcgttcac ttgaaatgaa cccgctccag ctttattgtc 
     4441 atacctgcca tcaggcaatt ttgttgccgt attgatagag acagaggatg aacctgcatt 
     4501 tgccagcaca acgccatgtg agccgcgctg attcataaat atctggttgt ttccattcgg 
     4561 gttcgagagt tcctcaggct gtccagccat cacattgtga aatctattga ccgcagtgat 
     4621 agcctgatct tcaaataaag cactcccgcg atcgcctatt tggcttttcc ccgggaacct 
     4681 cacaccattt ccgcctccct caggtctgga aaagaaaaga ggcgtactgc ctgaacgaga 
     4741 agctatcacc gcccagccta aacggatatc atcatcgctc atccatgtcg acgctctccc 
     4801 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc 
     4861 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct 
     4921 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc 
     4981 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg 
     5041 gccacgatgc gtccggcgta gaggatctgg agctgtaata taaaaacctt cttcaactaa 
     5101 cggggcaggt tagtgacatt agaaaaccga ctgtaaaaag tacagtcggc attatctcat 
     5161 attataaaag ccagtcatta ggcctatctg acaattcctg aatagagttc ataaacaatc 
     5221 ctgcatgata accatcacaa acagaatgat gtacctgtaa agatagcggt aaatatattg 
     5281 aattaccttt attaatgaat tttcctgctg taataatggg tagaaggtaa ttactattat 
     5341 tattgatatt taagttaaac ccagtaaatg aagtccatgg aataatagaa agagaaaaag 
     5401 cattttcagg tataggtgtt ttgggaaaca atttccccga accattatat ttctctacat 
     5461 cagaaaggta taaatcataa aactctttga agtcattctt tacaggagtc caaataccag 
     5521 agaatgtttt agatacacca tcaaaaattg tataaagtgg ctctaactta tcccaataac 
     5581 ctaactctcc gtcgctattg taaccagttc taaaagctgt atttgagttt atcacccttg 
     5641 tcactaagaa aataaatgca gggtaaaatt tatatccttc ttgttttatg tttcggtata 
     5701 aaacactaat atcaatttct gtggttatac taaaagtcgt ttgttggttc aaataatgat 
     5761 taaatatctc ttttctcttc caattgtcta aatcaatttt attaaagttc atttgatatg 
     5821 cctcctaaat ttttatctaa agtgaattta ggaggcttac ttgtctgctt tcttcattag 
     5881 aatcaatcct tttttaaaag tcaatattac tgtaacataa atatatattt taaaaatatc 
     5941 ccactttatc caattttcgt ttgttgaact aatgggtgct ttagttgaag aataaagacc 
     6001 acattaaaaa atgtggtctt ttgtgttttt ttaaaggatt tgagcgtagc gaaaaatcct 
     6061 tttctttctt atcttgataa taagggtaac tattgccgat gataagctgt caaacatga 
//
