LOCUS       pMG7                    5528 bp    DNA     circular     21-JUN-2013
DEFINITION  LMBP 8458.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8458
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 21-JUN-2013
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|663938180|
COMMENT     VNTDBDATE|665402584|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pMG7|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     misc_feature    complement(470..1126)
                     /vntifkey="21"
                     /label=5'\UTR\of\amyE
     CDS             1335..2195
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      2736..3138
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        3230..3313
                     /vntifkey="30"
                     /label=lac\promoter
     misc_feature    complement(3396..4200)
                     /vntifkey="21"
                     /label=3'\UTR\of\amyE
     CDS             complement(4571..5221)
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     promoter        184..220
                     /vntifkey="30"
                     /label=cggR\promoter
     misc_feature    240..335
                     /vntifkey="21"
                     /label=5'\UTR\of\gapA
BASE COUNT     1511 a      1238 c      1200 g      1579 t 
ORIGIN
        1 gaattctttg gataaaatga aaacgcttta atgaaacagc cctttctacg ggaagggctg 
       61 tttatattgg gatgcaccat ttggcgcttt ctgtataaga taaagatata taggataaaa 
      121 tattgctgga taaaacgacg cggcatgaaa actctgcgaa tattgtcgat gaattggctc 
      181 ttaacagttg aataaacaat tccaccctgt taaaataatt aaagaaagca gaaatctgca 
      241 gaagttatta agggatgaat aatccctcaa tataaatatc tctcacttat ttaaaggagg 
      301 aaagcttcat gtggagccac ccgcagttcg aaaaaggtac ccggggatcc tctagagtcc 
      361 tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggca 
      421 ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta attctgcgtg 
      481 acatcccatc gatcagacca gtttttaatt tgtgtgtttc catgtgtcca gtttggaata 
      541 ctcttaacct cattggaaat cgcggcataa tcactggtgg tatgattgat gaccgcgtca 
      601 acaatgacct ttatgccata ttcttcagcg gctgcacaca tttctttaaa ttcttgttca 
      661 gtacctaagt aacggttgcc aatttgatac gatgtcggct gatacagcca gtaccagttc 
      721 gacatgcttt tatctccttg attcccttcc tttacttggt taatcggaga tgtctgaatg 
      781 gctgtatatc ctgcatcatg aatatccttc atattgtgtt ttaacgtatt gaacgaccaa 
      841 ttccatgcat gaagaatggt tccgcttttg atcgacggtg ctgtaagctc attcgatttg 
      901 ttcgccgttt cagcactcgc agccgccggt cctgccagaa ccaaatgaaa cagcaataaa 
      961 aatccagcga ataacggcag taaagaggtt ttgaatcgtt ttgcaaacat tcttgacact 
     1021 ccttatttga ttttttgaag acttacttcg gagtcaaaaa tccctcttac ttcattcttc 
     1081 cgcttcctcc tttcaaaccg atgtgaagac tggagaattt tgttaattct tgaagacgaa 
     1141 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga 
     1201 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 
     1261 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 
     1321 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 
     1381 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 
     1441 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 
     1501 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 
     1561 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 
     1621 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 
     1681 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 
     1741 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 
     1801 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 
     1861 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 
     1921 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 
     1981 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 
     2041 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 
     2101 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 
     2161 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 
     2221 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 
     2281 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 
     2341 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 
     2401 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 
     2461 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 
     2521 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 
     2581 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 
     2641 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 
     2701 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 
     2761 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 
     2821 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 
     2881 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 
     2941 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 
     3001 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 
     3061 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 
     3121 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 
     3181 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 
     3241 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 
     3301 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg 
     3361 attacgccaa gctaatcggc ggccgcatcg atgcaggctc ccatcatgtt tatagatata 
     3421 agcatttacc tggctccaat gattcggatt ttgatagccg atggttttgg ccgacgctgg 
     3481 atctctttta acaaaactgt atttctcggt cctcgttaca ccatcactgt tcgttccttt 
     3541 taacatgatg gtgtatgttt tgccaaatgg atctcctttt ccgattgtga attgatctcc 
     3601 atccttaaac gccgtctctg gtccattatt gatttgataa acggcttttg ttgtattcgc 
     3661 atctgcacgc aaggtaatcg tcagttgatc attgaaagaa tgtgttacac ctgttttgta 
     3721 attctcaagg aaaacatgag gcgcttttgc aatatcatca ggataaagca cagctacaga 
     3781 cctggcattg atcgtgcctg tcagtttacc atcgttcact tgaaatgaac ccgctccagc 
     3841 tttattgtca tacctgccat caggcaattt tgttgccgta ttgatagaga cagaggatga 
     3901 acctgcattt gccagcacaa cgccatgtga gccgcgctga ttcataaata tctggttgtt 
     3961 tccattcggg ttcgagagtt cctcaggctg tccagccatc acattgtgaa atctattgac 
     4021 cgcagtgata gcctgatctt caaataaagc actcccgcga tcgcctattt ggcttttccc 
     4081 cgggaacctc acaccatttc cgcctccctc aggtctggaa aagaaaagag gcgtactgcc 
     4141 tgaacgagaa gctatcaccg cccagcctaa acggatatca tcatcgctca tccatgtcga 
     4201 cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg 
     4261 agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc 
     4321 acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc 
     4381 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg 
     4441 gtgatgccgg ccacgatgcg tccggcgtag aggatctgga gctgtaatat aaaaaccttc 
     4501 ttcaactaac ggggcaggtt agtgacatta gaaaaccgac tgtaaaaagt acagtcggca 
     4561 ttatctcata ttataaaagc cagtcattag gcctatctga caattcctga atagagttca 
     4621 taaacaatcc tgcatgataa ccatcacaaa cagaatgatg tacctgtaaa gatagcggta 
     4681 aatatattga attaccttta ttaatgaatt ttcctgctgt aataatgggt agaaggtaat 
     4741 tactattatt attgatattt aagttaaacc cagtaaatga agtccatgga ataatagaaa 
     4801 gagaaaaagc attttcaggt ataggtgttt tgggaaacaa tttccccgaa ccattatatt 
     4861 tctctacatc agaaaggtat aaatcataaa actctttgaa gtcattcttt acaggagtcc 
     4921 aaataccaga gaatgtttta gatacaccat caaaaattgt ataaagtggc tctaacttat 
     4981 cccaataacc taactctccg tcgctattgt aaccagttct aaaagctgta tttgagttta 
     5041 tcacccttgt cactaagaaa ataaatgcag ggtaaaattt atatccttct tgttttatgt 
     5101 ttcggtataa aacactaata tcaatttctg tggttatact aaaagtcgtt tgttggttca 
     5161 aataatgatt aaatatctct tttctcttcc aattgtctaa atcaatttta ttaaagttca 
     5221 tttgatatgc ctcctaaatt tttatctaaa gtgaatttag gaggcttact tgtctgcttt 
     5281 cttcattaga atcaatcctt ttttaaaagt caatattact gtaacataaa tatatatttt 
     5341 aaaaatatcc cactttatcc aattttcgtt tgttgaacta atgggtgctt tagttgaaga 
     5401 ataaagacca cattaaaaaa tgtggtcttt tgtgtttttt taaaggattt gagcgtagcg 
     5461 aaaaatcctt ttctttctta tcttgataat aagggtaact attgccgatg ataagctgtc 
     5521 aaacatga 
//
