ID pCR3-GW-GST-C standard; circular DNA; ; 7412 BP. DT 5-DEC-2013 DE LMBP 8374. CC Accession number: LMBP 8374 CC (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) CC Last modified: 05-DEC-2013 CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT terminator complement(4463..4532) FT /vntifkey="43" FT /label=HSV-TK\polyA FT rep_origin complement(5571..5646) FT /vntifkey="33" FT /label=SV40\ori FT promoter complement(5603..5884) FT /vntifkey="29" FT /label=SV40\early\promoter FT CDS complement(5963..6823) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin 6955..7410 FT /vntifkey="33" FT /label=f1\ori FT CDS complement(4723..5511) FT /vntifkey="4" FT /label=Tn5\neomycin\resistance FT rep_origin complement(3419..3745) FT /vntifkey="33" FT /label=pMB1\ori FT promoter 638..654 FT /vntifkey="30" FT /label=T7\promoter FT terminator 3171..3395 FT /vntifkey="43" FT /label=BGH\polyA FT promoter 10..593 FT /vntifkey="29" FT /label=hCMV-IE\promoter\and\enhancer FT attenuator complement(722..846) FT /vntifkey="0" FT /label=attR1 FT promoter 854..906 FT /vntifkey="30" FT /label=lacUV5\promoter FT CDS 955..1614 FT /vntifkey="4" FT /label=chloramphenicol\resistance FT CDS 1956..2261 FT /vntifkey="4" FT /label=ccdB FT attenuator 2302..2426 FT /vntifkey="0" FT /label=attR2 FT CDS 2458..3111 FT /vntifkey="4" FT /label=GST FT promoter complement(3129..3145) FT /vntifkey="30" FT /label=SP6\promoter SQ Sequence 7412 BP; 1870 A; 1784 C; 1840 G; 1918 t; gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 60 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 120 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 180 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg 240 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 300 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 360 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 420 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 480 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 540 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc 600 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga 660 cccaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc 720 aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt 780 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc 840 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat 900 gtgtggattt tgagttagga tccggcgaga ttttcaggag ctaaggaagc taaaatggag 960 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt 1020 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg 1080 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt 1140 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg 1200 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt 1260 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa 1320 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg 1380 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat 1440 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag 1500 gtgctgatgc cgctggcgat tcaggttcat catgccgtct gtgatggctt ccatgtcggc 1560 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct 1620 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt 1680 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtgt gctatgaagc 1740 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt 1800 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga 1860 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa 1920 cggctctttt gctgacgaga acagggactg gtgaaatgca gtttaaggtt tacacctata 1980 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg 2040 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg 2100 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg 2160 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg 2220 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc tccgttatac 2280 acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta 2340 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct 2400 cgttcagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg ctcgagctcc 2460 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa 2520 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga 2580 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat 2640 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg 2700 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt 2760 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt 2820 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat 2880 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt 2940 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt 3000 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct 3060 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatg atctagaggg 3120 ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc 3180 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 3240 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 3300 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 3360 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagtgg 3420 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 3480 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 3540 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 3600 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 3660 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 3720 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 3780 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 3840 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 3900 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 3960 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 4020 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 4080 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 4140 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 4200 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 4260 tatatgagta acctgaggct atggcagggc ctgccgcccc gacgttggct gcgagccctg 4320 ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc 4380 cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt 4440 tatgaacaaa cgacccaaca ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc 4500 gggttccttc cggtattgtc tccttccgtg tttcagttag cctcccccta gggtgggcga 4560 agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga 4620 ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt 4680 tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag 4740 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa 4800 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc 4860 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt 4920 ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc 4980 gggcatgctc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttg 5040 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt 5100 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc 5160 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg 5220 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc 5280 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt 5340 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg 5400 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct 5460 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga 5520 tcctcatcct gtctcttgat cgatctttgc aaaagcctag gcctccaaaa aagcctcctc 5580 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa 5640 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg 5700 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc 5760 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt 5820 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc 5880 acagctggtt ctttccgcct caggactctt cctttttcaa taaatcaatc taaagtatat 5940 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 6000 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 6060 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 6120 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 6180 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 6240 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 6300 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 6360 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 6420 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 6480 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 6540 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 6600 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 6660 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 6720 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 6780 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 6840 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 6900 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc 6960 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 7020 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 7080 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 7140 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 7200 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 7260 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 7320 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 7380 attttaacaa aatattaacg cttacaattt ac 7412 //