ID pGFP-N-GW standard; circular DNA; ; 6448 BP. DT 28-OCT-2014 DE LMBP 8367. XX CC Accession number: LMBP 8367 CC (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) CC Last modified: 28-OCT-2014 CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT rep_origin 39..365 FT /vntifkey="33" FT /label=pMB1\ori FT promoter 372..939 FT /vntifkey="29" FT /label=hCMV-IE\promoter\and\enhancer FT attenuator complement(1719..1843) FT /vntifkey="0" FT /label=attR1 FT promoter 1851..1903 FT /vntifkey="30" FT /label=lacUV5\promoter FT CDS 1952..2611 FT /vntifkey="4" FT /label=chloramphenicol\resistance FT CDS 2953..3258 FT /vntifkey="4" FT /label=ccdB FT attenuator 3299..3423 FT /vntifkey="0" FT /label=attR2 FT terminator 3585..3681 FT /vntifkey="43" FT /label=SV40\polyA\early FT rep_origin complement(3735..4190) FT /vntifkey="33" FT /label=f1\ori FT promoter 4342..4623 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin 4580..4655 FT /vntifkey="33" FT /label=SV40\ori FT CDS 4715..5509 FT /vntifkey="4" FT /label=Tn5\neomycin\resistance FT terminator 5700..5769 FT /vntifkey="43" FT /label=HSV-TK\polyA FT promoter 4191..4286 FT /vntifkey="30" FT /label=ampicillin\resistance\promoter FT RBS 4287..4321 FT /vntifkey="32" FT /label=5'\UTR\of\amp,\incl.\RBS FT terminator complement(3585..3681) FT /vntifkey="43" FT /label=SV40\polyA\late FT CDS 984..1700 FT /vntifkey="4" FT /label=EGFP SQ Sequence 6448 BP; 1625 A; 1631 C; 1649 G; 1543 t; gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 60 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 120 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 180 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 240 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 300 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 360 ccgccatgca ttagttatta atagtaatca attacggggt cattagttca tagcccatat 420 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 480 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 540 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 600 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 660 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 720 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 780 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 840 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 900 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc 960 cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg 1020 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 1080 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 1140 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca 1200 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct 1260 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 1320 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 1380 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 1440 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 1500 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 1560 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca 1620 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 1680 gcatggacga gctgtacaag tccggactca gatcatcaac aagtttgtac aaaaaagctg 1740 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac 1800 agactacata atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac 1860 cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc 1920 ggcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac 1980 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca 2040 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa 2100 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca 2160 tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc 2220 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca 2280 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa 2340 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg 2400 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt 2460 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca 2520 ggttcatcat gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca 2580 gtactgcgat gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag 2640 ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta 2700 tacccgaagt atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac 2760 agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca 2820 caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg 2880 aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca 2940 gggactggtg aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg 3000 tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg 3060 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc 3120 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc 3180 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg 3240 atgttctggg gaatataaat gtcaggctcc gttatacaca gccagtctgc aggtcgacca 3300 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta 3360 atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt 3420 ggttgatgat ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga 3480 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt 3540 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt 3600 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 3660 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 3720 gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg 3780 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa 3840 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa 3900 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg 3960 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa 4020 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa 4080 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct 4140 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac 4200 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4260 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4320 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 4380 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 4440 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 4500 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 4560 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 4620 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 4680 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 4740 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 4800 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 4860 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg 4920 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 4980 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 5040 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 5100 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 5160 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 5220 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg 5280 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 5340 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 5400 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 5460 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg 5520 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc 5580 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 5640 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa 5700 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat 5760 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac 5820 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc 5880 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca 5940 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt 6000 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 6060 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 6120 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 6180 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 6240 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 6300 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 6360 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 6420 cagcggtcgg gctgaacggg gggttcgt 6448 //