LOCUS       pDsRed-N-GW             6402 bp    DNA     circular     28-OCT-2014
DEFINITION  LMBP 8365.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8365
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 28-OCT-2014
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|713009832|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pDsRed-N-GW|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pDsRed-N-GW" 0 6402 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 1 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 150 0 
COMMENT        1 0 (CHomObj 1 0 0 3 75) 
COMMENT        (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) (CWordArray) 
COMMENT        (CStringList "HpaI" "NcoI" "NheI" "NotI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
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COMMENT            0 5442 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HpaI" "gttaac" 4 0 1 131 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 3935 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 4821 0 0 "")) 
COMMENT       (CObList 
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COMMENT            (CStringList) 1 1 1 1 "complement(4694..4818)") 
COMMENT        #5=(CFSignal (CObList) "lacUV5 promoter" 30 0 0 4826 4878 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4826..4878") 
COMMENT        #6=(CFSignal (CObList) "chloramphenicol resistance" 4 0 0 4927 5586 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4927..5586") 
COMMENT        #7=(CFSignal (CObList) "ccdB" 4 0 0 5928 6233 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "5928..6233") 
COMMENT        #8=(CFSignal (CObList) "attR2" 0 0 0 6274 6398 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "6274..6398") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA early" 43 0 0 109 205 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "109..205") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA late" 43 0 1 109 205 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(109..205)") 
COMMENT        #11=(CFSignal (CObList) "ampicillin resistance promoter" 30 0 0 715 810 
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COMMENT        #12=(CFSignal (CObList) "5' UTR of amp, incl. RBS" 32 0 0 811 845 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "811..845") 
COMMENT        #13=(CFSignal (CObList) "SV40 early promoter" 29 0 0 866 1147 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "866..1147") 
COMMENT        #14=(CFSignal (CObList) "SV40 ori" 33 0 0 1104 1179 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1104..1179") 
COMMENT        #15=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 1239 2033 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1239..2033") 
COMMENT        #16=(CFSignal (CObList) "HSV-TK polyA" 43 0 0 2224 2293 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2224..2293") 
COMMENT        #17=(CFSignal (CObList) "pMB1 ori" 33 0 0 3011 3337 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3011..3337") 
COMMENT        #18=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 3344 3911 
COMMENT             0 (CStringList) (CStringList) 1 1 1 1 "3344..3911") 
COMMENT        #19=(CFSignal (CObList) "mDsRed" 4 0 0 3956 4630 0 (CStringList) 
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COMMENT       (CObList) 
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COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #22=(CFSignal (CObList) "Last modified: 28-OCT-2014" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pDsRed-N-GW'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6402 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 22-OCT-2014" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "" 1))) "LMBP 8365") 
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COMMENT                          "Attenuator (2 total)") 
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COMMENT                          "CDS (4 total)") 
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COMMENT                                 "hCMV-IE promoter and enhancer"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                          "Promoter Prokaryotic (2 total)") 
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COMMENT                    "Restriction/Methylation Map") 
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COMMENT                            "is calculated as an AVERAGE similarity" 1))) 
COMMENT                    "Motifs (both strands)") 
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FEATURES             Location/Qualifiers
     attenuator      complement(4694..4818)
                     /vntifkey="0"
                     /label=attR1
     promoter        4826..4878
                     /vntifkey="30"
                     /label=lacUV5\promoter
     CDS             4927..5586
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             5928..6233
                     /vntifkey="4"
                     /label=ccdB
     attenuator      6274..6398
                     /vntifkey="0"
                     /label=attR2
     terminator      109..205
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(109..205)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(259..714)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        715..810
                     /vntifkey="30"
                     /label=ampicillin\resistance\promoter
     RBS             811..845
                     /vntifkey="32"
                     /label=5'\UTR\of\amp,\incl.\RBS
     promoter        866..1147
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      1104..1179
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             1239..2033
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      2224..2293
                     /vntifkey="43"
                     /label=HSV-TK\polyA
     rep_origin      3011..3337
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        3344..3911
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     CDS             3956..4630
                     /vntifkey="4"
                     /label=mDsRed
BASE COUNT     1603 a      1620 c      1645 g      1534 t 
ORIGIN
        1 gggatccacc ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt 
       61 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 
      121 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 
      181 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 
      241 caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt 
      301 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat 
      361 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat 
      421 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac 
      481 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc 
      541 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga 
      601 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 
      661 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg 
      721 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 
      781 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 
      841 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc 
      901 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 
      961 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 
     1021 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 
     1081 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 
     1141 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 
     1201 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 
     1261 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 
     1321 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 
     1381 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat 
     1441 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 
     1501 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 
     1561 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 
     1621 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 
     1681 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 
     1741 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc 
     1801 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 
     1861 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 
     1921 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 
     1981 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 
     2041 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 
     2101 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 
     2161 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac 
     2221 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca 
     2281 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg 
     2341 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt 
     2401 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc 
     2461 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca 
     2521 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 
     2581 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 
     2641 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 
     2701 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 
     2761 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt 
     2821 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 
     2881 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 
     2941 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 
     3001 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 
     3061 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 
     3121 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 
     3181 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 
     3241 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 
     3301 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat 
     3361 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 
     3421 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 
     3481 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 
     3541 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 
     3601 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 
     3661 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 
     3721 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 
     3781 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 
     3841 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 
     3901 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatgga 
     3961 caacaccgag gacgtcatca aggagttcat gcagttcaag gtgcgcatgg agggctccgt 
     4021 gaacggccac tacttcgaga tcgagggcga gggcgagggc aagccctacg agggcaccca 
     4081 gaccgccaag ctgcaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc 
     4141 cccccagttc cagtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta 
     4201 catgaagctg tccttccccg agggcttcac ctgggagcgc tccatgaact tcgaggacgg 
     4261 cggcgtggtg gaggtgcagc aggactcctc cctgcaggac ggcaccttca tctacaaggt 
     4321 gaagttcaag ggcgtgaact tccccgccga cggccccgta atgcagaaga agactgccgg 
     4381 ctgggagccc tccaccgaga agctgtaccc ccaggacggc gtgctgaagg gcgagatctc 
     4441 ccacgccctg aagctgaagg acggcggcca ctacacctgc gacttcaaga ccgtgtacaa 
     4501 ggccaagaag cccgtgcagc tgcccggcaa ccactacgtg gactccaagc tggacatcac 
     4561 caaccacaac gaggactaca ccgtggtgga gcagtacgag cacgccgagg cccgccactc 
     4621 cggctcccag tccggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac 
     4681 cgcgggccca tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa 
     4741 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca 
     4801 acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc 
     4861 ggctcgtata atgtgtggat tttgagttag gatccggcga gattttcagg agctaaggaa 
     4921 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt 
     4981 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag 
     5041 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc 
     5101 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa 
     5161 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa 
     5221 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac 
     5281 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt 
     5341 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta 
     5401 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg 
     5461 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc 
     5521 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg 
     5581 gcgtaaagat ctggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct 
     5641 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt 
     5701 gtgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg 
     5761 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg 
     5821 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt 
     5881 tattgaaatg aacggctctt ttgctgacga gaacagggac tggtgaaatg cagtttaagg 
     5941 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta 
     6001 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata 
     6061 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga 
     6121 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc 
     6181 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag 
     6241 gctccgttat acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta 
     6301 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca 
     6361 ttttacgttt ctcgttcagc tttcttgtac aaagtggttg at 
//
