LOCUS       pYFP-C-GW               6446 bp    DNA     circular     11-JUN-2013
DEFINITION  LMBP 8362.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8362
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 11-JUN-2013
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|664538882|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pYFP-C-GW|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pYFP-C-GW" 0 6446 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "AgeI" "NcoI" "SacI" "XhoI" "PstI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 6446 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 2916 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 5 897 0 1947 0 2927 0 4736 
COMMENT            0 5439 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 1157 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1150 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 1175 0 2772 0 3137 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "SV40 polyA early" 43 0 0 3748 3844 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3748..3844") 
COMMENT        #6=(CFSignal (CObList) "f1 ori" 33 0 1 3898 4353 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3898..4353)") 
COMMENT        #7=(CFSignal (CObList) "SV40 early promoter" 29 0 0 4505 4786 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4505..4786") 
COMMENT        #8=(CFSignal (CObList) "SV40 ori" 33 0 0 4743 4818 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4743..4818") 
COMMENT        #9=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 4878 5672 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4878..5672") 
COMMENT        #10=(CFSignal (CObList) "HSV-TK polyA" 43 0 0 5863 5932 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5863..5932") 
COMMENT        #11=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 537 1104 
COMMENT             0 (CStringList) (CStringList) 1 1 1 1 "537..1104") 
COMMENT        #12=(CFSignal (CObList) "attR1" 0 0 1 1199 1323 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1199..1323)") 
COMMENT        #13=(CFSignal (CObList) "attR2" 0 0 0 2779 2903 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2779..2903") 
COMMENT        #14=(CFSignal (CObList) "lacUV5 promoter" 30 0 0 1327 1383 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1327..1383") 
COMMENT        #15=(CFSignal (CObList) "chloramphenicol resistance" 4 0 0 1432 2091 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1432..2091") 
COMMENT        #16=(CFSignal (CObList) "ccdB" 4 0 0 2433 2738 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2433..2738") 
COMMENT        #17=(CFSignal (CObList) "EYFP" 4 0 0 2928 3647 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2928..3647") 
COMMENT        #18=(CFSignal (CObList) "amp promoter" 30 0 0 4354 4449 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4354..4449") 
COMMENT        #19=(CFSignal (CObList) "amp RBS" 32 0 0 4450 4484 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4450..4484") 
COMMENT        #20=(CFSignal (CObList) "pMB1 ori" 33 0 0 204 530 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "204..530") 
COMMENT        #21=(CFSignal (CObList) "SV40 polyA late" 43 0 1 3748 3844 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3748..3844)")) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #22=(CFSignal (CObList) "Last modified: 11-JUN-2013" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #23=(CFSignal (CObList) "LMBP 8362" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #24=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #25=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pYFP-C-GW" 2) 5 
COMMENT                   "" 0 4) 
COMMENT              #26=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'pYFP-C-GW'" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 06/11/13 10:08" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6446 bp" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #34=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 11-JUN-2013" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #37=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #38=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #39=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #40=(CLinePar (CParagraph 0 (15 #22# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 11-JUN-2013") 
COMMENT                     #41=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #42=(CLinePar (CParagraph 0 (15 #23# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 8362"))) "Annotations") 
COMMENT              #43=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #44=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (7 0 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #45=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1199 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1199  End: 1323 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1199..1323)" 1))) "attR1") 
COMMENT                            #49=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2779 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2779  End: 2903" 1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2779..2903" 1))) "attR2"))) 
COMMENT                          "Attenuator (2 total)") 
COMMENT                     #53=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #54=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1432 (3 #15# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1432  End: 2091" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1432..2091" 1))) 
COMMENT                                 "chloramphenicol resistance") 
COMMENT                            #58=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2433 (3 #16# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2433  End: 2738" 1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2433..2738" 1))) "ccdB") 
COMMENT                            #62=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2928 (3 #17# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2928  End: 3647" 1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2928..3647" 1))) "EYFP") 
COMMENT                            #66=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4878 (3 #9# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4878  End: 5672" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4878..5672" 1))) 
COMMENT                                 "Tn5 neomycin resistance"))) "CDS (4 total)") 
COMMENT                     #70=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #71=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 537 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 537   End: 1104" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   537..1104" 1))) 
COMMENT                                 "hCMV-IE promoter and enhancer") 
COMMENT                            #75=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4505 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4505  End: 4786" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4505..4786" 1))) 
COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #79=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #80=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1327 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1327  End: 1383" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1327..1383" 1))) "lacUV5 promoter") 
COMMENT                            #84=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4354 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4354  End: 4449" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4354..4449" 1))) "amp promoter"))) 
COMMENT                          "Promoter Prokaryotic (2 total)") 
COMMENT                     #88=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 32 (7 32 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #89=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4450 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4450  End: 4484" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4450..4484" 1))) "amp RBS"))) 
COMMENT                          "RBS (1 total)") 
COMMENT                     #93=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #94=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 204 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 204   End: 530 " 1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   204..530" 1))) "pMB1 ori") 
COMMENT                            #98=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3898 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3898  End: 4353 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   complement(3898..4353)" 1))) 
COMMENT                                 "f1 ori") 
COMMENT                            #102=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 4743 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #103=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 4743  End: 4818" 1) 
COMMENT                                    #104=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #105=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   4743..4818" 1))) "SV40 ori"))) 
COMMENT                          "Replication Origin (3 total)") 
COMMENT                     #106=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #107=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3748 (3 #5# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #108=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3748  End: 3844" 1) 
COMMENT                                     #109=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3748..3844" 1))) 
COMMENT                                   "SV40 polyA early") 
COMMENT                             #111=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3748 (3 #21# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3748  End: 3844 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #113=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(3748..3844)" 1))) 
COMMENT                                   "SV40 polyA late") 
COMMENT                             #115=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5863 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5863  End: 5932" 1) 
COMMENT                                     #117=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #118=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   5863..5932" 1))) "HSV-TK polyA"))) 
COMMENT                           "Terminator (3 total)"))) "Feature Map") 
COMMENT              #119=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #120=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #121=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #122=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2916 " 1))))) "AgeI: 1 site") 
COMMENT                            1 5 "ACCGGT" "TGGCCA") 
COMMENT                      #123=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #124=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #125=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1157 " 1))))) "SacI: 1 site") 
COMMENT                            5 1 "GAGCTC" "CTCGAG") 
COMMENT                      #126=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #127=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #128=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1150 " 1))))) "XhoI: 1 site") 
COMMENT                            1 5 "CTCGAG" "GAGCTC") 
COMMENT                      #129=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #130=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #131=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1175 " 1) 
COMMENT                                      #132=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2772 " 1) 
COMMENT                                      #133=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3137 " 1))))) "PstI: 3 sites") 
COMMENT                            5 1 "CTGCAG" "GACGTC") 
COMMENT                      #134=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 5 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #135=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #136=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 897 " 1) 
COMMENT                                      #137=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1947 " 1) 
COMMENT                                      #138=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 2927 " 1) 
COMMENT                                      #139=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 4736 " 1) 
COMMENT                                      #140=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 5439 " 1))))) "NcoI: 5 sites") 
COMMENT                            1 5 "CCATGG" "GGTACC"))) 
COMMENT                    "Restriction/Methylation Map") 
COMMENT              #141=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 20 (0 0) 1 2 0 1 178) 
COMMENT                     (CObjectList 
COMMENT                      #142=(CRefLinePar 
COMMENT                            (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) 
COMMENT                             " 4484: pYFP-C-GW: PstI(3137) - PstI(1175)" 1) 4 "" 
COMMENT                            3137 1174) 
COMMENT                      #143=(CRefLinePar 
COMMENT                            (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) 
COMMENT                             " 1597: pYFP-C-GW: PstI(1175) - PstI(2772)" 1) 4 "" 
COMMENT                            1175 2771) 
COMMENT                      #144=(CRefLinePar 
COMMENT                            (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) 
COMMENT                             " 365: pYFP-C-GW: PstI(2772) - PstI(3137)" 1) 4 "" 
COMMENT                            2772 3136))) "Restriction Fragments")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #145=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #146=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #147=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #148=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
COMMENT          #149=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 
COMMENT                0 0) 
COMMENT          #150=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 0 0 0) 
COMMENT          #151=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT                0 0 0) 
COMMENT          #152=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #153=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #154=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #155=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #156=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #157=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #158=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #159=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #160=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #161=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #162=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #163=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) #164=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #165=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #166=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #167=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #168=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #169=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #170=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #171=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #172=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #173=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #174=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #175=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #176=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #177=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #178=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1 
COMMENT                65535 "@N" 0) 
COMMENT          #179=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #180=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 3342336) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #181=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #182=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #183=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #184=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #185=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #186=(CWidgetStyle "Promoter_E" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #187=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #188=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 10040064) 6 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #189=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #190=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #191=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 0) 2 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #192=(CWidgetStyle "Label 26" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #193=(CWidgetStyle "Shape GAP" 1 (LOGPEN 1 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #194=(CWidgetStyle "Label 27" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #195=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16750848 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #196=(CWidgetStyle "Label 28" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #197=(CWidgetStyle "Label 29" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #198=(CWidgetStyle "Mutation Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #199=(CWidgetStyle "Label 30" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #200=(CWidgetStyle "MSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 255 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #201=(CWidgetStyle "Label 34" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #202=(CWidgetStyle "Label 45" 1 (LOGPEN 0 0 3381657) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32896 1 5 "@N (@S)" 0) 
COMMENT          #203=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 39321) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #204=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 16724736) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)) 
COMMENT         0.164644 1.74233 0.164644 2.53336 
COMMENT         (15 (CShapeMapEntry 0 "Shape 5" 1 "Label 20") 9 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 
COMMENT          (CShapeMapEntry 0 "Shape 3" 1 "Label 20") 0 
COMMENT          (CShapeMapEntry 8 "Shape 20" 1 "Label 21") 30 
COMMENT          (CShapeMapEntry 0 "Shape 18" 1 "Label 20") 29 
COMMENT          (CShapeMapEntry 0 "Promoter_E" 1 "Label 20") 26 
COMMENT          (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 
COMMENT          (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 
COMMENT          (CShapeMapEntry 1 "Shape 13" 1 "Label 20") 21 
COMMENT          (CShapeMapEntry 5 "Line 2" 1 "Label 21") 43 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 30") 33 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Label 20") 32 
COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 21") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Label 27") 62 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Mutation Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 29") 50 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 28") 69 
COMMENT          (CShapeMapEntry 0 "Motif" 1 "MSite Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 23" 1 "Label 34") 68 
COMMENT          (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 
COMMENT          (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 40.3368 40.3368 39 39 0.1 
COMMENT         -3261) 1 1 0 1 1 
COMMENT        (mapper: 25.4741 -16.0622 40.3368 40.3368 0.01 10 -1 6446 6446 1 0 0) 
COMMENT        #205=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -1519 100) 
COMMENT              (CObjectList 
COMMENT               #206=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -815 100) 
COMMENT                     (CObjectList 
COMMENT                      #207=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #151# 29233404 0) 
COMMENT                             (LOGPEN 0 0 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27516 
COMMENT                             6.27316 1 0.0214037) 0.097319) 
COMMENT                      #208=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #148# 24488044 100) 
COMMENT                            (LOGPEN 0 0 3381657) 1 
COMMENT                            (LOGFONT 63 24 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.91 0 "pYFP-C-GW" "@N" 1 0 0.871165 0 -10 
COMMENT                            7.90841 1.56185 Nil) 
COMMENT                      #209=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #147# 29401236 100) 
COMMENT                            (LOGPEN 0 0 3381657) 1 
COMMENT                            (LOGFONT 73 27 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.74233 1.05 0 "6446 bp" "@L bp" 16 
COMMENT                            0 -0.871165 0 -10.8 4.78471 1.80976 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #210=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -882 100) 
COMMENT                     (CObjectList 
COMMENT                      #211=(CGroupWidget 
COMMENT                            (CWidget 0 (7 0 0) 1 2 0 0 Nil -260 100) 
COMMENT                            (CObjectList 
COMMENT                             #212=(CWideLine 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #195# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 (LOGBRUSH 0 16750848 0) 1 
COMMENT                                   4.98565 5.1073 1 0.082322) 
COMMENT                             #213=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #190# 1128482644 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "attR1" "@N" 1 
COMMENT                                   16.3224 4.14014 0 -10 2.15684 1.16519 #212#) 
COMMENT                             #214=(CWideLine 
COMMENT                                   (CWidget 0 (3 #13# 0) 1 2 0 0 #195# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 (LOGBRUSH 0 16750848 0) 1 
COMMENT                                   3.44801 3.56966 1 0.082322) 
COMMENT                             #215=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #190# 393238 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "attR2" "@N" 1 
COMMENT                                   6.19829 -14.0061 0 -10 2.15684 1.16519 #214#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #216=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -892 100) 
COMMENT                            (CObjectList 
COMMENT                             #217=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #9# 0) 1 2 0 0 #149# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    0.753249 1.52694 1 0.082322) 0.8 1.8 0) 
COMMENT                             #218=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #189# 28904424 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Tn5 neomycin resistance" "@N" 1 -18.6877 
COMMENT                                   6.64009 0 -10 10.09 1.16519 #217#) 
COMMENT                             #219=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #15# 0) 1 2 0 0 #149# 1250 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    4.23824 4.88055 1 0.082322) 0.8 1.8 0) 
COMMENT                             #220=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #189# 1255 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "chloramphenicol resistance" "@N" 1 19.1001 
COMMENT                                   -2.84009 0 -10 11.0073 1.16519 #219#) 
COMMENT                             #221=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #16# 0) 1 2 0 0 #149# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    3.60859 3.90639 1 0.082322) 0.8 1.8 0) 
COMMENT                             #222=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #189# 29406084 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "ccdB" "@N" 1 
COMMENT                                   8.72871 -11.042 0 -10 2.15684 1.16519 #221#) 
COMMENT                             #223=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #17# 0) 1 2 0 0 #149# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    2.72396 3.42466 1 0.082322) 0.8 1.8 0) 
COMMENT                             #224=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #189# 29409852 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "EYFP" "@N" 1 
COMMENT                                   -2.2085 -15.32 0 -10 2.256 1.16519 #223#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #225=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -1371 100) 
COMMENT                            (CObjectList 
COMMENT                             #226=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #186# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    1.6155 1.88994 1 0.082322) 0.8 1.8 0) 
COMMENT                             #227=(CLabel (CWidget 0 (0 0) 1 2 0 0 #189# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 early promoter" "@N" 1 -19.1835 
COMMENT                                   -3.30418 0 -10 8.35465 1.16519 #226#) 
COMMENT                             #228=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #11# 0) 1 2 0 0 #186# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    5.19878 5.75156 1 0.082322) 0.8 1.8 0) 
COMMENT                             #229=(CLabel (CWidget 0 (0 0) 1 2 0 0 #189# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "hCMV-IE promoter and enhancer" "@N" 1 
COMMENT                                   17.7467 11.761 0 -10 13.065 1.16519 #228#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #230=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -337 100) 
COMMENT                            (CObjectList 
COMMENT                             #231=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #191# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 2 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    4.92726 4.98273 1 0.082322) 0.8 1.8 0) 
COMMENT                             #232=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #189# 1094796097 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "lacUV5 promoter" "@N" 1 19.0165 1.97636 0 
COMMENT                                   -10 6.7928 1.16519 #231#) 
COMMENT                             #233=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #18# 0) 1 2 0 0 #191# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 2 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    1.94346 2.03689 1 0.082322) 0.8 1.8 0) 
COMMENT                             #234=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #189# 27876412 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "amp promoter" 
COMMENT                                   "@N" 1 -16.8612 -6.78884 0 -10 5.55324 
COMMENT                                   1.16519 #233#)) (CObjectList)) 
COMMENT                      #235=(CGroupWidget 
COMMENT                            (CWidget 32 (7 32 0) 1 2 0 0 Nil -391 100) 
COMMENT                            (CObjectList 
COMMENT                             #236=(CScratch 
COMMENT                                   (CWidget 0 (3 #19# 0) 1 2 0 0 #166# 0 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 1 1.94346 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #237=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #190# 1414808387 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "amp RBS" "@N" 1 
COMMENT                                   -16.3379 -5.46796 0 -10 3.74348 1.16519 #236#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #238=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -704 100) 
COMMENT                            (CObjectList 
COMMENT                             #239=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #6# 0) 1 2 0 0 #161# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 
COMMENT                                    1.0068 2.03689 2.48066 1 0.082322) 0.8 1.8 1) 
COMMENT                             #240=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #189# 24852420 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "f1 ori" "@N" 1 
COMMENT                                   -12.7298 -10.1922 0 -10 2.15684 1.16519 #239#) 
COMMENT                             #241=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #161# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 
COMMENT                                    0.835356 1.58435 1.65832 1 0.082322) 0.8 1.8 
COMMENT                                   0) 
COMMENT                             #242=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #189# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SV40 ori" "@N" 
COMMENT                                   1 -17.86 1.09776 0 -10 3.56994 1.16519 #241#) 
COMMENT                             #243=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #20# 0) 1 2 0 0 #161# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    5.75739 6.07563 1 0.082322) 0.8 1.8 0) 
COMMENT                             #244=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #189# -742 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 6.8892 13.7512 0 -10 3.56994 1.16519 #243#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #245=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -393 100) 
COMMENT                            (CObjectList 
COMMENT                             #246=(CLine 
COMMENT                                   (CWidget 0 (3 #5# 0) 1 2 0 0 #152# 0 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.882132 2.53224 
COMMENT                                   2.62664) 
COMMENT                             #247=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #199# 28008924 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA early" "@N" 1 -11.4297 -13.3546 0 
COMMENT                                   -10 7.11509 1.16519 #246#) 
COMMENT                             #248=(CLine 
COMMENT                                   (CWidget 0 (3 #10# 0) 1 2 0 0 #152# 534 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 0.50022 
COMMENT                                   0.568343) 
COMMENT                             #249=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #199# 2082602612 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "HSV-TK polyA" 
COMMENT                                   "@N" 1 -9.34376 11.4456 0 -10 5.87553 1.16519 
COMMENT                                   #248#) 
COMMENT                             #250=(CLine 
COMMENT                                   (CWidget 0 (3 #21# 0) 1 2 0 0 #152# 0 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.74953 2.53224 
COMMENT                                   2.62664) 
COMMENT                             #251=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #199# 29412572 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA late" "@N" 1 -9.33273 -15.1574 0 
COMMENT                                   -10 6.54489 1.16519 #250#)) (CObjectList))) 
COMMENT                     (CObjectList)) 
COMMENT               #252=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -1304 100) 
COMMENT                     (CObjectList 
COMMENT                      #253=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -1245 100) 
COMMENT                            (CObjectList 
COMMENT                             #254=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #204# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 3.43634 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #255=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #201# 28809268 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Age}I (2916)" "@N (@S)" 5 6.68718 
COMMENT                                   -15.5997 0 -10 4.09056 0.99165 #254#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #256=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #2# 0) 1 2 0 0 Nil -1175 100) 
COMMENT                            (CObjectList 
COMMENT                             #257=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #204# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 5.14818 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #258=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #201# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Sac}I (1157)" "@N (@S)" 5 15.6813 
COMMENT                                   6.88106 0 -10 4.01618 0.99165 #257#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #259=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -116 100) 
COMMENT                            (CObjectList 
COMMENT                             #260=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #204# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 5.15499 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #261=(CLabel (CWidget 0 (0 0) 1 2 0 0 #201# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Xho}I (1150)" "@N (@S)" 5 15.7044 
COMMENT                                   8.27631 0 -10 4.16493 0.99165 #260#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #262=(CGroupWidget 
COMMENT                            (CWidget 3 (10 #4# 0) 1 2 0 0 Nil -651 100) 
COMMENT                            (CObjectList 
COMMENT                             #263=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #203# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 5.13066 1.9 0.082322 1) 
COMMENT                             #264=(CLabel (CWidget 0 (0 0) 1 2 0 0 #202# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Pst}I (1175)" "@N (@S)" 5 16.2993 
COMMENT                                   5.60977 0 -10 3.91702 0.99165 #263#) 
COMMENT                             #265=(CScratch 
COMMENT                                   (CWidget 2 (1 #4# 2) 1 2 0 0 #203# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 3.57647 1.9 0.082322 1) 
COMMENT                             #266=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #202# 1195852611 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Pst}I (2772)" "@N (@S)" 5 7.85875 
COMMENT                                   -12.6108 0 -10 3.91702 0.99165 #265#) 
COMMENT                             #267=(CScratch 
COMMENT                                   (CWidget 3 (1 #4# 3) 1 2 0 0 #203# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 3.22126 1.9 0.082322 1) 
COMMENT                             #268=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #202# 29324964 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Pst}I (3137)" "@N (@S)" 5 3.28592 
COMMENT                                   -18.415 0 -10 3.91702 0.99165 #267#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #269=(CGroupWidget 
COMMENT                            (CWidget 5 (10 #1# 0) 1 2 0 0 Nil -256 100) 
COMMENT                            (CObjectList 
COMMENT                             #270=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #203# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 5.40121 1.9 0.082322 1) 
COMMENT                             #271=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #202# 25158244 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Nco}I (897)" "@N (@S)" 5 13.6566 
COMMENT                                   10.1922 0 -10 3.74348 0.99165 #270#) 
COMMENT                             #272=(CScratch 
COMMENT                                   (CWidget 2 (1 #1# 2) 1 2 0 0 #203# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 4.37936 1.9 0.082322 1) 
COMMENT                             #273=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #202# 30613564 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Nco}I (1947)" "@N (@S)" 5 17.1176 
COMMENT                                   -5.89635 0 -10 4.16493 0.99165 #272#) 
COMMENT                             #274=(CScratch 
COMMENT                                   (CWidget 3 (1 #1# 3) 1 2 0 0 #203# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 3.42563 1.9 0.082322 1) 
COMMENT                             #275=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #202# 27969748 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Nco}I (2927)" "@N (@S)" 5 6.36174 
COMMENT                                   -16.8214 0 -10 4.16493 0.99165 #274#) 
COMMENT                             #276=(CScratch 
COMMENT                                   (CWidget 4 (1 #1# 4) 1 2 0 0 #203# 690 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 1.66513 1.9 0.082322 1) 
COMMENT                             #277=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #202# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Nco}I (4736)" "@N (@S)" 5 -18.5591 
COMMENT                                   -0.495825 0 -10 4.16493 0.99165 #276#) 
COMMENT                             #278=(CScratch 
COMMENT                                   (CWidget 5 (1 #1# 5) 1 2 0 0 #203# 690 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 0.980976 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #279=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #202# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Nco}I (5439)" "@N (@S)" 5 -15.2938 
COMMENT                                   8.95262 0 -10 4.16493 0.99165 #278#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #280=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1033 100) 
COMMENT                     (CObjectList 
COMMENT                      #281=(CLabel 
COMMENT                            (CWidget 0 (15 #22# 0) 1 2 0 0 #174# 28806036 100) 
COMMENT                            (LOGPEN 268786312 28806048 27929448) 28806060 
COMMENT                            (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "Last modified: 11-JUN-2013" 
COMMENT                            "Last modified: 11-JUN-2013" 65535 0.0743738 
COMMENT                            -4.28889 0 -10 10.9329 1.06602 Nil) 
COMMENT                      #282=(CLabel 
COMMENT                            (CWidget 0 (15 #23# 0) 1 2 0 0 #174# 29237852 100) 
COMMENT                            (LOGPEN 28051544 29237864 24691632) 29237876 
COMMENT                            (LOGFONT 63 24 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 8362" 
COMMENT                            "LMBP 8362" 65535 0.099165 -2.65266 0 -10 6.84239 
COMMENT                            1.56185 Nil)) (CObjectList)) 
COMMENT               #283=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -895 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 1196705607 
COMMENT       (CStringList) 1094795585 1195852099 
COMMENT       (CObList 
COMMENT        #284=(CSampFrag "pYFP-C-GW" 4484 3137 "PstI" 1175 "PstI" (LOGPEN 0 0 0) 
COMMENT              1.2092e-316 1.57488e-301) 
COMMENT        #285=(CSampFrag "pYFP-C-GW" 1597 1175 "PstI" 2772 "PstI" (LOGPEN 0 0 0) 
COMMENT              1.42934e-316 4.0648e-300) 
COMMENT        #286=(CSampFrag "pYFP-C-GW" 365 2772 "PstI" 3137 "PstI" (LOGPEN 0 0 0) 
COMMENT              1.41897e-316 3.30544e-287))))
FEATURES             Location/Qualifiers
     terminator      3748..3844
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      complement(3898..4353)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        4505..4786
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      4743..4818
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             4878..5672
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      5863..5932
                     /vntifkey="43"
                     /label=HSV-TK\polyA
     promoter        537..1104
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     attenuator      complement(1199..1323)
                     /vntifkey="0"
                     /label=attR1
     attenuator      2779..2903
                     /vntifkey="0"
                     /label=attR2
     promoter        1327..1383
                     /vntifkey="30"
                     /label=lacUV5\promoter
     CDS             1432..2091
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             2433..2738
                     /vntifkey="4"
                     /label=ccdB
     CDS             2928..3647
                     /vntifkey="4"
                     /label=EYFP
     promoter        4354..4449
                     /vntifkey="30"
                     /label=amp\promoter
     RBS             4450..4484
                     /vntifkey="32"
                     /label=amp\RBS
     rep_origin      204..530
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(3748..3844)
                     /vntifkey="43"
                     /label=SV40\polyA\late
BASE COUNT     1622 a      1632 c      1650 g      1542 t 
ORIGIN
        1 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 
       61 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 
      121 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 
      181 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 
      241 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 
      301 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 
      361 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 
      421 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 
      481 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattagt 
      541 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt 
      601 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg 
      661 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg 
      721 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt 
      781 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg 
      841 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg 
      901 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt 
      961 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac 
     1021 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg 
     1081 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg 
     1141 actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccatcaac 
     1201 aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa 
     1261 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact 
     1321 atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg 
     1381 tggattttga gttaggatcc ggcgagattt tcaggagcta aggaagctaa aatggagaaa 
     1441 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag 
     1501 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc 
     1561 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt 
     1621 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg 
     1681 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca 
     1741 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat 
     1801 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt 
     1861 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg 
     1921 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg 
     1981 ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca tgtcggcaga 
     2041 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta aagatctgga 
     2101 tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata 
     2161 agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtgtgct atgaagcagc 
     2221 gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa 
     2281 tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg 
     2341 ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg 
     2401 ctcttttgct gacgagaaca gggactggtg aaatgcagtt taaggtttac acctataaaa 
     2461 gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc 
     2521 gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac 
     2581 tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca 
     2641 gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca 
     2701 tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc gttatacaca 
     2761 gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc 
     2821 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt 
     2881 tcagctttct tgtacaaagt ggttgatggg atccaccggt cgccaccatg gtgagcaagg 
     2941 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 
     3001 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 
     3061 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct 
     3121 tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct 
     3181 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 
     3241 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 
     3301 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 
     3361 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 
     3421 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 
     3481 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc 
     3541 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 
     3601 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcgact 
     3661 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc 
     3721 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat 
     3781 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 
     3841 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa 
     3901 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt 
     3961 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag 
     4021 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg 
     4081 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat 
     4141 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc 
     4201 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga 
     4261 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac 
     4321 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc 
     4381 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 
     4441 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa 
     4501 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 
     4561 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 
     4621 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 
     4681 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 
     4741 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 
     4801 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag 
     4861 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt 
     4921 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 
     4981 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 
     5041 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg 
     5101 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 
     5161 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 
     5221 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 
     5281 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 
     5341 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 
     5401 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga 
     5461 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 
     5521 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 
     5581 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 
     5641 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga 
     5701 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag 
     5761 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct 
     5821 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat 
     5881 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg 
     5941 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc 
     6001 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag 
     6061 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc 
     6121 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 
     6181 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 
     6241 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 
     6301 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 
     6361 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 
     6421 tactgtcctt ctagtgtagc cgtagt 
//
