LOCUS       pCOIN-DV                7613 bp    DNA     circular UNA 19-JAN-2016
DEFINITION  LMBP 8192.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8192
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 19-JAN-2016
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|667047483|
COMMENT     VNTDBDATE|-11070086|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCOIN DV|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #3=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 20 0 5527 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "PvuII" "cagctg" 4 0 4 1328 0 3692 0 6933 0 
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FEATURES             Location/Qualifiers
     misc_feature    35..277
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    278..520
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    1070..1117
                     /vntifkey="21"
                     /label=FRT\mutant
     CDS             complement(2323..3183)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_feature    3801..3848
                     /vntifkey="21"
                     /label=FRT
     CDS             complement(5740..6045)
                     /vntifkey="4"
                     /label=ccdB
     CDS             complement(6387..7046)
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     rep_origin      complement(1380..1782)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(1205..1288)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        539..1046
                     /vntifkey="29"
                     /label=mPGK1\promoter
     misc_feature    complement(4031..4064)
                     /vntifkey="21"
                     /label=loxP
     terminator      4751..4847
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4751..4847)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      5005..5101
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      5259..5355
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(5005..5101)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      complement(5259..5355)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     misc_feature    complement(5444..5477)
                     /vntifkey="21"
                     /label=loxP
     attenuator      complement(5575..5699)
                     /vntifkey="0"
                     /label=attR4
     attenuator      7155..7279
                     /vntifkey="0"
                     /label=attR3
     promoter        complement(7095..7147)
                     /vntifkey="30"
                     /label=lacUV5\promoter
     terminator      7344..7568
                     /vntifkey="43"
                     /label=BGH\polyA
     misc_feature    3865..3968
                     /vntifkey="21"
                     /label=SA
BASE COUNT     1878 a      1967 c      1810 g      1956 t         2 others
ORIGIN
        1 cgcgccgggc cctcgcgact cgaggaataa attcgagctc acggggacag ccccccccca 
       61 aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg 
      121 ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc 
      181 ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg 
      241 agcctgcaga cacctggggg atacggggaa aaagcttgag ctcacgggga cagccccccc 
      301 ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc 
      361 cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac 
      421 agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct 
      481 ttgagcctgc agacacctgg gggatacggg gaaaaagctt gtcgacgaat aaattcaatt 
      541 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 
      601 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 
      661 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 
      721 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 
      781 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 
      841 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 
      901 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 
      961 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 
     1021 ctcttcctca tctccgggcc tttcgacctg caatcgccgc caccatggag aagttcctat 
     1081 tccgaagttc ctattcttca aaaggtatag gaacttccga tcgggatccc caatcctgca 
     1141 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 
     1201 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 
     1261 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 
     1321 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 
     1381 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     1441 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     1501 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     1561 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     1621 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     1681 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     1741 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     1801 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     1861 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     1921 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     1981 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 
     2041 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     2101 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     2161 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 
     2221 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 
     2281 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 
     2341 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 
     2401 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 
     2461 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 
     2521 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 
     2581 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 
     2641 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 
     2701 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 
     2761 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 
     2821 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 
     2881 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 
     2941 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 
     3001 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 
     3061 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 
     3121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 
     3181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 
     3241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 
     3301 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 
     3361 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 
     3421 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 
     3481 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 
     3541 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 
     3601 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 
     3661 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 
     3721 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga 
     3781 attccacaaa agtgcgatcg gaagttccta ttccgaagtt cctattctct agaaagtata 
     3841 ggaacttctt aattaaggga tctgtagggc gcagtagtcc agggtttcct tgatgatgtc 
     3901 atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc 
     3961 tttccagtgg ggatcgacgg tatcgtagag tcgaggccgc tctagaacta gtggatccgg 
     4021 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc cctcgacctg 
     4081 caggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta 
     4141 gcagccccgc tggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat 
     4201 ccaccggtag cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc 
     4261 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat 
     4321 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg 
     4381 ggtaggatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt cctcccactc 
     4441 atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga ttcccacttt 
     4501 gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag aacgagatca 
     4561 gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt ctaattccat 
     4621 cagaagcttg cagatctgcg actctagagg atctgcgact ctagaggatc ataatcagcc 
     4681 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc 
     4741 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt 
     4801 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 
     4861 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctgc gactctagag 
     4921 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca 
     4981 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc 
     5041 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 
     5101 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat 
     5161 ctgcgactct agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta 
     5221 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt 
     5281 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 
     5341 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 
     5401 tatcatgtct ggatccccat caagctgatc cggaaccctt aatataactt cgtataatgt 
     5461 atgctatacg aagttattag gtccctcgac ctgcagccca agctagctta tcgataccgt 
     5521 cgacnctcga gnttttccca gtcacgacgt tgtaaaacga cggccagtga attatcaact 
     5581 ttgtatagaa aagttgaacg agaaacgtaa aatgatataa atatcaatat attaaattag 
     5641 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg 
     5701 tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc agaacatcag 
     5761 gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat 
     5821 aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat 
     5881 atgcaccacc gggtaaagtt cacgggggac tttatctgac agcagacgtg cactggccag 
     5941 ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag 
     6001 acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt 
     6061 tctcgtcggc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc atcccttcct 
     6121 gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc atggttgtgc 
     6181 ttaccgaacc ggagatattg acatcatata tgccttgagc aactgatagc tgtcgctgtc 
     6241 aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact tcgggtatac 
     6301 atatcagtat atattcttat accgcaaaaa tcagcgcgca aatacgcata ctgttatctg 
     6361 gcttttagta agccggatcc tctagattac gccccgccct gccactcatc gcagtactgt 
     6421 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga 
     6481 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg 
     6541 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag 
     6601 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt 
     6661 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg 
     6721 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg 
     6781 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga 
     6841 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc 
     6901 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga 
     6961 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg 
     7021 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgacgga 
     7081 tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa gcctggggtg 
     7141 cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt 
     7201 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa 
     7261 ctttattata catagttgat aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa 
     7321 acgagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc 
     7381 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 
     7441 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 
     7501 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg 
     7561 ctctatggct tctgaggcgg aaagaaccag ctggggctcg atcctctagt tgg 
//
