LOCUS       pLenti6(puro)V5-SHP1-C453S        9028 bp    DNA     circular SYN 11-SEP-2009
DEFINITION  LMBP 8072.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|527708457|
COMMENT     VNTDBDATE|529062239|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLenti6(puro)V5-SHP1-C453S|
COMMENT     VNTAUTHORNAME|kkersse|
COMMENT     VNTOAUTHORNAME|Invitrogen|
COMMENT     VNTOAUTHORTEL|(760)603-7200|
COMMENT     VNTOAUTHORFAX|(760)602-6500|
COMMENT     VNTOAUTHOREML|tech_service@invitrogen.com|
COMMENT     VNTOAUTHORWWW|http://www.invitrogen.com|
COMMENT     VNTOAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTOAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTOAUTHORAD3|Carlsbad, CA 92008, U.S.A.|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "Foreign object. Author: kkersse. Original author: Invitrogen" 
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FEATURES             Location/Qualifiers
     LTR             695..928
                     /invitrogen="1390000"
                     /vntifkey="19"
                     /label=deltaU3/3'\LTR
     CDS             2089..2949
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     promoter        1990..2088
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     CDS             1..603
                     /invitrogen="1050000"
                     /vntifkey="4"
                     /label=Puro(R)
     primer          6448..6468
                     /invitrogen="1930000"
                     /vntifkey="27"
                     /label=CMV\forward\primer
     promoter        5983..6565
                     /invitrogen="2170000"
                     /vntifkey="29"
                     /label=CMV\promoter
     LTR             694..747
                     /invitrogen="1410000"
                     /vntifkey="19"
                     /label=deltaU3
     rep_origin      1503..1958
                     /invitrogen="2520000"
                     /vntifkey="33"
                     /label=f1\origin
     LTR             4404..4584
                     /invitrogen="1430000"
                     /vntifkey="19"
                     /label=HIV-1\5'\LTR
     LTR             748..928
                     /invitrogen="1430000"
                     /vntifkey="19"
                     /label=HIV-1\3'\LTR
     misc_binding    4695..4739
                     /invitrogen="1780000"
                     /vntifkey="20"
                     /label=HIV-1\psi\packaging\signal
     rep_origin      3094..3767
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     protein_bind    5249..5482
                     /invitrogen="2440000"
                     /vntifkey="31"
                     /label=HIV-1\Rev\response\element
     promoter        4175..4403
                     /invitrogen="2270000"
                     /vntifkey="29"
                     /label=RSV\promoter
     promoter        4175..4584
                     /invitrogen="2280000"
                     /vntifkey="29"
                     /label=RSV/5'\LTR\hybrid\promoter
     polyA_signal    1000..1130
                     /invitrogen="1840000"
                     /vntifkey="25"
                     /label=SV40\pA
     primer          complement(8573..8593)
                     /invitrogen="2140000"
                     /vntifkey="27"
                     /label=V5\reverse\primer
     CDS             8564..8605
                     /invitrogen="1350000"
                     /vntifkey="4"
                     /label=V5\epitope
     splicing_signal 4694..4694
                     /invitrogen="123000012"
                     /vntifkey="38"
                     /label=splice\donor
     splicing_signal 5830..5830
                     /invitrogen="123000013"
                     /vntifkey="38"
                     /label=splice\acceptor
     splicing_signal 5858..5858
                     /invitrogen="123000014"
                     /vntifkey="38"
                     /label=splice\acceptor
     TATA_signal     6483..6486
                     /invitrogen="123000002"
                     /vntifkey="41"
                     /label=TATA\box
     promoter        8660..8969
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             8615..8623
                     /invitrogen="123000001"
                     /vntifkey="4"
                     /label=3\stops
     misc_feature    6686..8470
                     /vntifkey="21"
                     /label=SHP-1\WT
     mutation        8042..8044
                     /vntifkey="62"
                     /label=Ser\in\SHP-1\C453S\inactivate\mutant
     misc_signal     8494..8503
                     /vntifkey="87"
                     /label=attB
     misc_signal     6622..6631
                     /vntifkey="87"
                     /label=attB
BASE COUNT     2295 a      2289 c      2407 g      2037 t 
ORIGIN
        1 atggccaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc 
       61 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg 
      121 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 
      181 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 
      241 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggct cgcgcatggc cgagttgagc 
      301 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 
      361 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 
      421 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgctggggt gcccgccttc 
      481 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 
      541 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 
      601 tgagctaagc acaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta 
      661 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga 
      721 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 
      781 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 
      841 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 
      901 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 
      961 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat 
     1021 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 
     1081 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta 
     1141 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     1201 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 
     1261 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta 
     1321 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa 
     1381 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 
     1441 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 
     1501 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 
     1561 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 
     1621 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 
     1681 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 
     1741 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 
     1801 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 
     1861 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 
     1921 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat 
     1981 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 
     2041 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 
     2101 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 
     2161 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 
     2221 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 
     2281 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 
     2341 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 
     2401 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 
     2461 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 
     2521 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 
     2581 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 
     2641 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 
     2701 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 
     2761 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 
     2821 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 
     2881 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 
     2941 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 
     3001 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 
     3061 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 
     3121 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 
     3181 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 
     3241 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc 
     3301 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 
     3361 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 
     3421 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 
     3481 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 
     3541 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 
     3601 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 
     3661 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 
     3721 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 
     3781 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 
     3841 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 
     3901 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 
     3961 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 
     4021 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 
     4081 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct 
     4141 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt 
     4201 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc 
     4261 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga 
     4321 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta 
     4381 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc 
     4441 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 
     4501 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 
     4561 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa 
     4621 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga 
     4681 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga 
     4741 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg 
     4801 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga 
     4861 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat 
     4921 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa 
     4981 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc 
     5041 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc 
     5101 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat 
     5161 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg 
     5221 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag 
     5281 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt 
     5341 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt 
     5401 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat 
     5461 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca 
     5521 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca 
     5581 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa 
     5641 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat 
     5701 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca 
     5761 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga 
     5821 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg 
     5881 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca 
     5941 ttcgattagt gaacggatct cgacggtatc gataagcttg ggagttccgc gttacataac 
     6001 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 
     6061 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 
     6121 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 
     6181 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 
     6241 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc 
     6301 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc 
     6361 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa 
     6421 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg 
     6481 tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct 
     6541 gttttgacct ccatagaaga caccgactct agaggatcca ctagtccagt gtggtggaat 
     6601 tctgcagata tcaacaagtt tgtacaaaaa agcaggctct ttaaaggaac caattcagtc 
     6661 gactggatcc ggtaccgaat tcaccatggt gaggtggttt caccgagacc tcagtgggct 
     6721 ggatgcagag accctgctca agggccgagg tgtccacggt agcttcctgg ctcggcccag 
     6781 tcgcaagaac cagggtgact tctcgctctc cgtcagggtg ggggatcagg tgacccatat 
     6841 tcggatccag aactcagggg atttctatga cctgtatgga ggggagaagt ttgcgactct 
     6901 gacagagctg gtggagtact acactcagca gcagggtgtc ctgcaggacc gcgacggcac 
     6961 catcatccac ctcaagtacc cgctgaactg ctccgatccc actagtgaga ggtggtacca 
     7021 tggccacatg tctggcgggc aggcagagac gctgctgcag gccaagggcg agccctggac 
     7081 gtttcttgtg cgtgagagcc tcagccagcc tggagacttc gtgctttctg tgctcagtga 
     7141 ccagcccaag gctggcccag gctccccgct cagggtcacc cacatcaagg tcatgtgcga 
     7201 gggtggacgc tacacagtgg gtggtttgga gaccttcgac agcctcacgg acctggtgga 
     7261 gcatttcaag aagacgggga ttgaggaggc ctcaggcgcc tttgtctacc tgcggcagcc 
     7321 gtactatgcc acgagggtga atgcggctga cattgagaac cgagtgttgg aactgaacaa 
     7381 gaagcaggag tccgaggata cagccaaggc tggcttctgg gaggagtttg agagtttgca 
     7441 gaagcaggag gtgaagaact tgcaccagcg tctggaaggg cagcggccag agaacaaggg 
     7501 caagaaccgc tacaagaaca ttctcccctt tgaccacagc cgagtgatcc tgcagggacg 
     7561 ggacagtaac atccccgggt ccgactacat caatgccaac tacatcaaga accagctgct 
     7621 aggccctgat gagaacgcta agacctacat cgccagccag ggctgtctgg aggccacggt 
     7681 caatgacttc tggcagatgg cgtggcagga gaacagccgt gtcatcgtca tgaccacccg 
     7741 agaggtggag aaaggccgga acaaatgcgt cccatactgg cccgaggtgg gcatgcagcg 
     7801 tgcttatggg ccctactctg tgaccaactg cggggagcat gacacaaccg aatacaaact 
     7861 ccgtacctta caggtctccc cgctggacaa tggagacctg attcgggaga tctggcatta 
     7921 ccagtacctg agctggcccg accatggggt ccccagtgag cctgggggtg tcctcagctt 
     7981 cctggaccag atcaaccagc ggcaggaaag tctgcctcac gcagggccca tcatcgtgca 
     8041 ctgcagcgcc ggcatcggcc gcacaggcac catcattgtc atcgacatgc tcatggagaa 
     8101 catctccacc aagggcctgg actgtgacat tgacatccag aagaccatcc agatggtgcg 
     8161 ggcgcagcgc tcgggcatgg tgcagacgga ggcgcagtac aagttcatct acgtggccat 
     8221 cgcccagttc attgaaacca ctaagaagaa gctggaggtc ctgcagtcgc agaagggcca 
     8281 ggagtcggag tacgggaaca tcacctatcc cccagccatg aagaatgccc atgccaaggc 
     8341 ctcccgcacc tcgtccaaac acaaggagga tgtgtatgag aacctgcaca ctaagaacaa 
     8401 gagggaggag aaagtgaaga agcagcggtc agcagacaag gagaagagca agggttccct 
     8461 caagaggaag cactcgagat atctagaccc agctttcttg tacaaagtgg ttgatatcca 
     8521 gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa 
     8581 ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa tgagtttgga attaattctg 
     8641 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat 
     8701 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 
     8761 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 
     8821 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 
     8881 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 
     8941 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 
     9001 cattttcgga tctgatcagc acgtgacc 
//
